Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206252433> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W4206252433 abstract "Abstract Background: MicroRNAs (miRNAs), which are single-stranded small RNAs approximately 21 to 23 bases long, are involved in the regulation of cell proliferation, apoptosis, and lipid metabolism/fatty acid metabolism, and play important roles in the differentiation of the liver and the maintenance of its morphology and function MiRNAs are also closely related to the occurrence, development, treatment, and prognosis of liver diseases. Fibrotic Liver is a chronic process of the intrahepatic damage–repair response. The mixed effects of these pathogenic factors result in an abnormal comprehensive proliferation of fibrous tissue in the liver, diffused production and deposition of the extracellular matrix (ECM), and an imbalance in ECM synthesis and degradation. These lead to secondary inflammatory responses in the liver and the reversible pathological process of self-healing. Methods: Liver tissues were obtained from 3 patients with CHB with liver histopathological fibrosis grade ≥ S2, 2 patients with CHB with fibrosis grade < S2, and 1 healthy individual with a normal liver. The high-throughput miRNA microarray technique was used for miRNA expression analysis. The screening criteria for differential miRNA expression were a fold-change ≥ 2 and P < 0.005. The miRNAs with significantly different expression levels were verified by quantitative real-time PCR (qPCR), and the target gene functions were predicted by Gene Ontology (GO) and pathway analyses. Finally, an miRNA-gene network map was constructed. Results: In the CHB groups of tissues, 34 miRNAs with ≥ 2-fold difference in expression were found, of which 18 were upregulated and 16 were downregulated. qPCR was used to verify these miRNAs that showed significant differences, with the results showing consistency with the microarray results, indicating that the miRNA microarray results were credible. Bioinformatics analysis results demonstrate that some of the key upregulated miRNAs found in the network map were hsa-miR-125b-2-3p, hsa-miR-4639-3p, hsa-miR-4764-3p, and hsa-miR-3133, whereas some of the key downregulated ones were hsa-miR-1297, hsa-miR-154-5p, hsa-miR-3183 and hsa-miR-663a. Conclusion: In patients with CHB, the miRNA expression profile changes significantly with the severity of fibrotic liver. The development of fibrotic liver may be related to the miRNA-mediated regulation of cell development, metabolism, and apoptosis, and the positive/negative regulation of cell processes.This study was approved by the ethics committee of general hospital of Ningxia medical university (2015-134)." @default.
- W4206252433 created "2022-01-26" @default.
- W4206252433 creator A5004349988 @default.
- W4206252433 creator A5004656133 @default.
- W4206252433 creator A5014889218 @default.
- W4206252433 creator A5029752940 @default.
- W4206252433 creator A5037574072 @default.
- W4206252433 creator A5040360009 @default.
- W4206252433 creator A5072453373 @default.
- W4206252433 creator A5076427704 @default.
- W4206252433 creator A5091854106 @default.
- W4206252433 date "2020-07-08" @default.
- W4206252433 modified "2023-09-26" @default.
- W4206252433 title "Differential Expression of MicroRNAs in Fibrotic Liver Tissues of Patients with Chronic Hepatitis B" @default.
- W4206252433 doi "https://doi.org/10.21203/rs.3.rs-33117/v1" @default.
- W4206252433 hasPublicationYear "2020" @default.
- W4206252433 type Work @default.
- W4206252433 citedByCount "0" @default.
- W4206252433 crossrefType "posted-content" @default.
- W4206252433 hasAuthorship W4206252433A5004349988 @default.
- W4206252433 hasAuthorship W4206252433A5004656133 @default.
- W4206252433 hasAuthorship W4206252433A5014889218 @default.
- W4206252433 hasAuthorship W4206252433A5029752940 @default.
- W4206252433 hasAuthorship W4206252433A5037574072 @default.
- W4206252433 hasAuthorship W4206252433A5040360009 @default.
- W4206252433 hasAuthorship W4206252433A5072453373 @default.
- W4206252433 hasAuthorship W4206252433A5076427704 @default.
- W4206252433 hasAuthorship W4206252433A5091854106 @default.
- W4206252433 hasBestOaLocation W42062524331 @default.
- W4206252433 hasConcept C104317684 @default.
- W4206252433 hasConcept C126322002 @default.
- W4206252433 hasConcept C127561419 @default.
- W4206252433 hasConcept C134018914 @default.
- W4206252433 hasConcept C142724271 @default.
- W4206252433 hasConcept C145059251 @default.
- W4206252433 hasConcept C150194340 @default.
- W4206252433 hasConcept C18431079 @default.
- W4206252433 hasConcept C186836561 @default.
- W4206252433 hasConcept C189165786 @default.
- W4206252433 hasConcept C2778772119 @default.
- W4206252433 hasConcept C2779018089 @default.
- W4206252433 hasConcept C2779134260 @default.
- W4206252433 hasConcept C2780559512 @default.
- W4206252433 hasConcept C2992208098 @default.
- W4206252433 hasConcept C4733338 @default.
- W4206252433 hasConcept C48023723 @default.
- W4206252433 hasConcept C502942594 @default.
- W4206252433 hasConcept C54355233 @default.
- W4206252433 hasConcept C71924100 @default.
- W4206252433 hasConcept C8415881 @default.
- W4206252433 hasConcept C86803240 @default.
- W4206252433 hasConcept C95444343 @default.
- W4206252433 hasConceptScore W4206252433C104317684 @default.
- W4206252433 hasConceptScore W4206252433C126322002 @default.
- W4206252433 hasConceptScore W4206252433C127561419 @default.
- W4206252433 hasConceptScore W4206252433C134018914 @default.
- W4206252433 hasConceptScore W4206252433C142724271 @default.
- W4206252433 hasConceptScore W4206252433C145059251 @default.
- W4206252433 hasConceptScore W4206252433C150194340 @default.
- W4206252433 hasConceptScore W4206252433C18431079 @default.
- W4206252433 hasConceptScore W4206252433C186836561 @default.
- W4206252433 hasConceptScore W4206252433C189165786 @default.
- W4206252433 hasConceptScore W4206252433C2778772119 @default.
- W4206252433 hasConceptScore W4206252433C2779018089 @default.
- W4206252433 hasConceptScore W4206252433C2779134260 @default.
- W4206252433 hasConceptScore W4206252433C2780559512 @default.
- W4206252433 hasConceptScore W4206252433C2992208098 @default.
- W4206252433 hasConceptScore W4206252433C4733338 @default.
- W4206252433 hasConceptScore W4206252433C48023723 @default.
- W4206252433 hasConceptScore W4206252433C502942594 @default.
- W4206252433 hasConceptScore W4206252433C54355233 @default.
- W4206252433 hasConceptScore W4206252433C71924100 @default.
- W4206252433 hasConceptScore W4206252433C8415881 @default.
- W4206252433 hasConceptScore W4206252433C86803240 @default.
- W4206252433 hasConceptScore W4206252433C95444343 @default.
- W4206252433 hasLocation W42062524331 @default.
- W4206252433 hasOpenAccess W4206252433 @default.
- W4206252433 hasPrimaryLocation W42062524331 @default.
- W4206252433 hasRelatedWork W1879733234 @default.
- W4206252433 hasRelatedWork W2008565169 @default.
- W4206252433 hasRelatedWork W2032879513 @default.
- W4206252433 hasRelatedWork W2072797841 @default.
- W4206252433 hasRelatedWork W2132025459 @default.
- W4206252433 hasRelatedWork W2207156387 @default.
- W4206252433 hasRelatedWork W2356489734 @default.
- W4206252433 hasRelatedWork W2392221252 @default.
- W4206252433 hasRelatedWork W3016705944 @default.
- W4206252433 hasRelatedWork W4250790143 @default.
- W4206252433 isParatext "false" @default.
- W4206252433 isRetracted "false" @default.
- W4206252433 workType "article" @default.