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- W4206265557 abstract "This chapter contains sections titled: Introduction Materials and methods Human serum samples Preparation of C. albicans protoplast lysates Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) Immunoblot analysis Peptide sample preparation for MS and MS/MS analyses In-gel digestion Desalting Mass spectrometric analysis (MS and MS/MS) Peptide mapping by MALDI-TOF-MS Peptide fragmentation and sequencing by nanoESI-IT-MS Database searching Results Mapping of C. albicans immunogenic proteins Overall 2-D C. albicans antigen recognition pattern Identification of C. albicans immunoreactive proteins by peptide mass fingerprinting (PMF) Peptide sequencing of C. albicans immunoreactive proteins Reference 2-DE C. albicans antigen pattern display on the Net Comparison of 2-D C. albicans antigen recognition patterns obtained with serum samples from patients with and without systemic candidiasis Differences in the 2-D C. albicans antigen recognition profile associated with infection progression Discussion C. albicans housekeeping enzymes can stimulate the human immune system during systemic candidiasis Heat shock proteins (Hsps) Metabolic proteins Elongation factors and ribosomal proteins Miscellaneous proteins Natural anti-Candida antibodies might be correlated with differentiation of the human immune response Serum levels of specific anti-Candida antibodies could be useful for the clinical follow-up of systemic candidiasis" @default.
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- W4206265557 date "2006-11-06" @default.
- W4206265557 modified "2023-09-27" @default.
- W4206265557 title "Proteomics‐based identification of novel <i>Candida albicans</i> antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies" @default.
- W4206265557 doi "https://doi.org/10.1002/9783527610099.ch16" @default.
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