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- W4206334098 abstract "Stem cell fate can be directed through the application of various external physical stimuli, enabling a controlled approach to targeted differentiation. Studies involving the use of dynamic mechanical cues driven by vibrational excitation to date have, however, been limited to low frequency (Hz to kHz) forcing over extended durations (typically continuous treatment for >7 days). Contrary to previous assertions that there is little benefit in applying frequencies beyond 1 kHz, we show here that high frequency MHz-order mechanostimulation in the form of nanoscale amplitude surface reflected bulk waves are capable of triggering differentiation of human mesenchymal stem cells from various donor sources toward an osteoblast lineage, with early, short time stimuli inducing long-term osteogenic commitment. More specifically, rapid treatments (10 min daily over 5 days) of the high frequency (10 MHz) mechanostimulation are shown to trigger significant upregulation in early osteogenic markers (RUNX2, COL1A1) and sustained increase in late markers (osteocalcin, osteopontin) through a mechanistic pathway involving piezo channel activation and Rho-associated protein kinase signaling. Given the miniaturizability and low cost of the devices, the possibility for upscaling the platform toward practical bioreactors, to address a pressing need for more efficient stem cell differentiation technologies in the pursuit of translatable regenerative medicine strategies, is ensivaged." @default.
- W4206334098 created "2022-01-26" @default.
- W4206334098 creator A5017582467 @default.
- W4206334098 creator A5038550094 @default.
- W4206334098 creator A5065373459 @default.
- W4206334098 date "2022-01-13" @default.
- W4206334098 modified "2023-10-18" @default.
- W4206334098 title "Short‐Duration High Frequency MegaHertz‐Order Nanomechanostimulation Drives Early and Persistent Osteogenic Differentiation in Mesenchymal Stem Cells" @default.
- W4206334098 cites W1963935144 @default.
- W4206334098 cites W1964875359 @default.
- W4206334098 cites W1965078317 @default.
- W4206334098 cites W1965933207 @default.
- W4206334098 cites W1968269253 @default.
- W4206334098 cites W1977720209 @default.
- W4206334098 cites W1978228345 @default.
- W4206334098 cites W1978489297 @default.
- W4206334098 cites W1983844949 @default.
- W4206334098 cites W1985229106 @default.
- W4206334098 cites W1987908267 @default.
- W4206334098 cites W1998696289 @default.
- W4206334098 cites W1999720993 @default.
- W4206334098 cites W2000465700 @default.
- W4206334098 cites W2006055704 @default.
- W4206334098 cites W2009286164 @default.
- W4206334098 cites W2012230802 @default.
- W4206334098 cites W2025492442 @default.
- W4206334098 cites W2030400425 @default.
- W4206334098 cites W2033440116 @default.
- W4206334098 cites W2040223443 @default.
- W4206334098 cites W2040788343 @default.
- W4206334098 cites W2041072969 @default.
- W4206334098 cites W2042230336 @default.
- W4206334098 cites W2048939228 @default.
- W4206334098 cites W2052052041 @default.
- W4206334098 cites W2053734323 @default.
- W4206334098 cites W2055873255 @default.
- W4206334098 cites W2060276230 @default.
- W4206334098 cites W2060986360 @default.
- W4206334098 cites W2061801043 @default.
- W4206334098 cites W2065233752 @default.
- W4206334098 cites W2071091332 @default.
- W4206334098 cites W2075871350 @default.
- W4206334098 cites W2081143491 @default.
- W4206334098 cites W2081892717 @default.
- W4206334098 cites W2103098975 @default.
- W4206334098 cites W2126092026 @default.
- W4206334098 cites W2127212119 @default.
- W4206334098 cites W2127337790 @default.
- W4206334098 cites W2127762602 @default.
- W4206334098 cites W2130804510 @default.
- W4206334098 cites W2134208433 @default.
- W4206334098 cites W2134982790 @default.
- W4206334098 cites W2140397612 @default.
- W4206334098 cites W2140915335 @default.
- W4206334098 cites W2144023435 @default.
- W4206334098 cites W2146046277 @default.
- W4206334098 cites W2151022917 @default.
- W4206334098 cites W2152077841 @default.
- W4206334098 cites W2154473013 @default.
- W4206334098 cites W2159639395 @default.
- W4206334098 cites W2160373069 @default.
- W4206334098 cites W2163780400 @default.
- W4206334098 cites W2164406135 @default.
- W4206334098 cites W2170106855 @default.
- W4206334098 cites W2231808169 @default.
- W4206334098 cites W2337958855 @default.
- W4206334098 cites W2346173838 @default.
- W4206334098 cites W2347117212 @default.
- W4206334098 cites W2407469927 @default.
- W4206334098 cites W2462720972 @default.
- W4206334098 cites W2466415363 @default.
- W4206334098 cites W2521229822 @default.
- W4206334098 cites W2582009831 @default.
- W4206334098 cites W2588390577 @default.
- W4206334098 cites W2604135765 @default.
- W4206334098 cites W2605496274 @default.
- W4206334098 cites W2613171263 @default.
- W4206334098 cites W2755592203 @default.
- W4206334098 cites W2767569544 @default.
- W4206334098 cites W2774927494 @default.
- W4206334098 cites W2775083937 @default.
- W4206334098 cites W2790742050 @default.
- W4206334098 cites W2793563162 @default.
- W4206334098 cites W2810890795 @default.
- W4206334098 cites W2820932019 @default.
- W4206334098 cites W2883781690 @default.
- W4206334098 cites W2893485895 @default.
- W4206334098 cites W2893715093 @default.
- W4206334098 cites W2904150188 @default.
- W4206334098 cites W2904858662 @default.
- W4206334098 cites W2941631697 @default.
- W4206334098 cites W2954202195 @default.
- W4206334098 cites W2956354975 @default.
- W4206334098 cites W2969308850 @default.
- W4206334098 cites W3012397626 @default.
- W4206334098 cites W3013895963 @default.
- W4206334098 cites W3023114435 @default.
- W4206334098 cites W3029384976 @default.
- W4206334098 cites W3089417973 @default.
- W4206334098 cites W3106556618 @default.