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• W4206366962 startingPage "111" @default.
• W4206366962 abstract "Publisher SummaryThis chapter discusses recent work on derivatization reactions for thin-layer chromatography (TLC) and gigh-pressure liquid chromatography (HPLC). Some TLC methods which may be applied to HPLC analysis are discussed. Dansylation is often used for the determination of free and N-terminal amino acids. Dansyl chloride (5-dimethylaminonaphthalene-1- sulfonyl chloride, DNS-Cl) reacts with the amino substituent of amino acids to form highly fluorescent derivatives. The method is particularly useful for the analysis of trace components due to the high sensitivity of the products. The derivatives are usually separated by TLC on various types of layers. Separations of DNS-amino acids by flat-bed techniques have been reviewed. Radiochemical derivatization is used for analysis of trace amounts of materials. The technique has the advantage of being both selective and sensitive. The main advantage is that the sample background does not cause interference in the detection as it does in most other methods and which necessitates some degree of clean-up. Also, the reactions used are those for normal derivatization procedures, the only difference being that the reagent is radiolabeled and that appropriate precautions are required for radioactive substances." @default.
• W4206366962 created "2022-01-25" @default.
• W4206366962 date "1976-01-01" @default.
• W4206366962 modified "2023-10-16" @default.
• W4206366962 title "Chapter 4 Applications" @default.
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