Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206382615> ?p ?o ?g. }
- W4206382615 abstract "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging." @default.
- W4206382615 created "2022-01-26" @default.
- W4206382615 creator A5001487212 @default.
- W4206382615 creator A5006700967 @default.
- W4206382615 creator A5021350396 @default.
- W4206382615 creator A5037031342 @default.
- W4206382615 creator A5053722819 @default.
- W4206382615 creator A5067447322 @default.
- W4206382615 creator A5070104377 @default.
- W4206382615 creator A5081825305 @default.
- W4206382615 creator A5086084093 @default.
- W4206382615 creator A5088471255 @default.
- W4206382615 creator A5091197463 @default.
- W4206382615 date "2022-01-11" @default.
- W4206382615 modified "2023-10-17" @default.
- W4206382615 title "Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells" @default.
- W4206382615 cites W1969103349 @default.
- W4206382615 cites W1995143570 @default.
- W4206382615 cites W2000057317 @default.
- W4206382615 cites W2059212792 @default.
- W4206382615 cites W2072895793 @default.
- W4206382615 cites W2078320842 @default.
- W4206382615 cites W2109281145 @default.
- W4206382615 cites W2162298934 @default.
- W4206382615 cites W2164742663 @default.
- W4206382615 cites W2257225795 @default.
- W4206382615 cites W2333673175 @default.
- W4206382615 cites W2396306987 @default.
- W4206382615 cites W2513876104 @default.
- W4206382615 cites W2553362503 @default.
- W4206382615 cites W2554260837 @default.
- W4206382615 cites W2581502995 @default.
- W4206382615 cites W2607225001 @default.
- W4206382615 cites W2616524931 @default.
- W4206382615 cites W2617667835 @default.
- W4206382615 cites W2771617786 @default.
- W4206382615 cites W2791547662 @default.
- W4206382615 cites W2804206940 @default.
- W4206382615 cites W2894519757 @default.
- W4206382615 cites W2902410320 @default.
- W4206382615 cites W2903604598 @default.
- W4206382615 cites W2921080611 @default.
- W4206382615 cites W2949580312 @default.
- W4206382615 cites W2953082998 @default.
- W4206382615 cites W2958054774 @default.
- W4206382615 cites W2976500897 @default.
- W4206382615 cites W3013005966 @default.
- W4206382615 cites W3014387643 @default.
- W4206382615 cites W3017611842 @default.
- W4206382615 cites W3091811870 @default.
- W4206382615 doi "https://doi.org/10.1038/s42003-021-02976-4" @default.
- W4206382615 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35017652" @default.
- W4206382615 hasPublicationYear "2022" @default.
- W4206382615 type Work @default.
- W4206382615 citedByCount "19" @default.
- W4206382615 countsByYear W42063826152022 @default.
- W4206382615 countsByYear W42063826152023 @default.
- W4206382615 crossrefType "journal-article" @default.
- W4206382615 hasAuthorship W4206382615A5001487212 @default.
- W4206382615 hasAuthorship W4206382615A5006700967 @default.
- W4206382615 hasAuthorship W4206382615A5021350396 @default.
- W4206382615 hasAuthorship W4206382615A5037031342 @default.
- W4206382615 hasAuthorship W4206382615A5053722819 @default.
- W4206382615 hasAuthorship W4206382615A5067447322 @default.
- W4206382615 hasAuthorship W4206382615A5070104377 @default.
- W4206382615 hasAuthorship W4206382615A5081825305 @default.
- W4206382615 hasAuthorship W4206382615A5086084093 @default.
- W4206382615 hasAuthorship W4206382615A5088471255 @default.
- W4206382615 hasAuthorship W4206382615A5091197463 @default.
- W4206382615 hasBestOaLocation W42063826151 @default.
- W4206382615 hasConcept C120665830 @default.
- W4206382615 hasConcept C121332964 @default.
- W4206382615 hasConcept C136009344 @default.
- W4206382615 hasConcept C136229726 @default.
- W4206382615 hasConcept C147080431 @default.
- W4206382615 hasConcept C1491633281 @default.
- W4206382615 hasConcept C15526883 @default.
- W4206382615 hasConcept C169274487 @default.
- W4206382615 hasConcept C171250308 @default.
- W4206382615 hasConcept C185592680 @default.
- W4206382615 hasConcept C192562407 @default.
- W4206382615 hasConcept C19275194 @default.
- W4206382615 hasConcept C41008148 @default.
- W4206382615 hasConcept C55493867 @default.
- W4206382615 hasConcept C57477423 @default.
- W4206382615 hasConcept C71924100 @default.
- W4206382615 hasConcept C76155785 @default.
- W4206382615 hasConcept C8673954 @default.
- W4206382615 hasConcept C91881484 @default.
- W4206382615 hasConceptScore W4206382615C120665830 @default.
- W4206382615 hasConceptScore W4206382615C121332964 @default.
- W4206382615 hasConceptScore W4206382615C136009344 @default.
- W4206382615 hasConceptScore W4206382615C136229726 @default.
- W4206382615 hasConceptScore W4206382615C147080431 @default.
- W4206382615 hasConceptScore W4206382615C1491633281 @default.
- W4206382615 hasConceptScore W4206382615C15526883 @default.
- W4206382615 hasConceptScore W4206382615C169274487 @default.
- W4206382615 hasConceptScore W4206382615C171250308 @default.
- W4206382615 hasConceptScore W4206382615C185592680 @default.
- W4206382615 hasConceptScore W4206382615C192562407 @default.