Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206483132> ?p ?o ?g. }
- W4206483132 endingPage "81" @default.
- W4206483132 startingPage "70" @default.
- W4206483132 abstract "Porcine epidemic diarrhea virus (PEDV) is the main cause of diarrhea, vomiting, and mortality in pigs, which results in devastating economic loss to the pig industry around the globe. In recent years, the advent of RNA-sequencing technologies has led to delineate host responses at late stages of PEDV infection; however, the comparative analysis of host responses to early-stage infection of virulent and avirulent PEDV strains is currently unknown. Here, using the BGI DNBSEQ RNA-sequencing, we performed global gene expression profiles of pig intestinal epithelial cells infected with virulent (GDS01) or avirulent (HX) PEDV strains for 3, 6, and 12 h. It was observed that over half of all significantly dysregulated genes in both infection groups exhibited a down-regulated expression pattern. Functional enrichment analyses indicated that the differentially expressed genes (DEGs) in the GDS01 group were predominantly related to autophagy and apoptosis, whereas the genes showing the differential expression in the HX group were strongly enriched in immune responses/inflammation. Among the DEGs, the functional association of TLR3 and IFIT2 genes with the HX and GDS01 strains replication was experimentally validated by TLR3 inhibition and IFIT2 overexpression systems in cultured cells. TLR3 expression was found to inhibit HX strain, but not GDS01 strain, replication by enhancing the IFIT2 expression in infected cells. In conclusion, our study highlights similarities and differences in gene expression patterns and cellular processes/pathways altered at the early-stage infection of PEDV virulent and avirulent strains. These findings may provide a foundation for establishing novel therapies to control PEDV infection." @default.
- W4206483132 created "2022-01-25" @default.
- W4206483132 creator A5013752180 @default.
- W4206483132 creator A5041182893 @default.
- W4206483132 creator A5051887031 @default.
- W4206483132 creator A5055501482 @default.
- W4206483132 creator A5057358632 @default.
- W4206483132 creator A5059797825 @default.
- W4206483132 creator A5064633706 @default.
- W4206483132 creator A5072273578 @default.
- W4206483132 creator A5074628946 @default.
- W4206483132 creator A5081442629 @default.
- W4206483132 date "2022-02-01" @default.
- W4206483132 modified "2023-10-04" @default.
- W4206483132 title "Genome-wide transcriptome analysis of porcine epidemic diarrhea virus virulent or avirulent strain-infected porcine small intestinal epithelial cells" @default.
- W4206483132 cites W1527927437 @default.
- W4206483132 cites W1966327575 @default.
- W4206483132 cites W1967021382 @default.
- W4206483132 cites W1999574084 @default.
- W4206483132 cites W2016457570 @default.
- W4206483132 cites W2017414846 @default.
- W4206483132 cites W2035494209 @default.
- W4206483132 cites W2040150395 @default.
- W4206483132 cites W2042345105 @default.
- W4206483132 cites W2050040190 @default.
- W4206483132 cites W2086955313 @default.
- W4206483132 cites W2089807622 @default.
- W4206483132 cites W2097957152 @default.
- W4206483132 cites W2102278945 @default.
- W4206483132 cites W2105637133 @default.
- W4206483132 cites W2106713698 @default.
- W4206483132 cites W2112533943 @default.
- W4206483132 cites W2117608012 @default.
- W4206483132 cites W2123060905 @default.
- W4206483132 cites W2132260239 @default.
- W4206483132 cites W2145441153 @default.
- W4206483132 cites W2165907695 @default.
- W4206483132 cites W2166574880 @default.
- W4206483132 cites W2166867592 @default.
- W4206483132 cites W2170551349 @default.
- W4206483132 cites W2315859740 @default.
- W4206483132 cites W2413584962 @default.
- W4206483132 cites W2602873486 @default.
- W4206483132 cites W2726784030 @default.
- W4206483132 cites W2735084243 @default.
- W4206483132 cites W2769035483 @default.
- W4206483132 cites W2774602704 @default.
- W4206483132 cites W2792406084 @default.
- W4206483132 cites W2794006249 @default.
- W4206483132 cites W2794573360 @default.
- W4206483132 cites W2891546414 @default.
- W4206483132 cites W2899735303 @default.
- W4206483132 cites W2903899730 @default.
- W4206483132 cites W2928883986 @default.
- W4206483132 cites W2941007250 @default.
- W4206483132 cites W2966575605 @default.
- W4206483132 cites W2981877750 @default.
- W4206483132 cites W2985352234 @default.
- W4206483132 cites W2986073447 @default.
- W4206483132 cites W3003217347 @default.
- W4206483132 cites W3004280078 @default.
- W4206483132 cites W3006891341 @default.
- W4206483132 cites W3012841297 @default.
- W4206483132 cites W3021590022 @default.
- W4206483132 cites W3025357968 @default.
- W4206483132 cites W3041822911 @default.
- W4206483132 cites W3092011752 @default.
- W4206483132 cites W3113358371 @default.
- W4206483132 cites W4248552215 @default.
- W4206483132 doi "https://doi.org/10.1016/j.virs.2022.01.011" @default.
- W4206483132 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35234615" @default.
- W4206483132 hasPublicationYear "2022" @default.
- W4206483132 type Work @default.
- W4206483132 citedByCount "3" @default.
- W4206483132 countsByYear W42064831322022 @default.
- W4206483132 countsByYear W42064831322023 @default.
- W4206483132 crossrefType "journal-article" @default.
- W4206483132 hasAuthorship W4206483132A5013752180 @default.
- W4206483132 hasAuthorship W4206483132A5041182893 @default.
- W4206483132 hasAuthorship W4206483132A5051887031 @default.
- W4206483132 hasAuthorship W4206483132A5055501482 @default.
- W4206483132 hasAuthorship W4206483132A5057358632 @default.
- W4206483132 hasAuthorship W4206483132A5059797825 @default.
- W4206483132 hasAuthorship W4206483132A5064633706 @default.
- W4206483132 hasAuthorship W4206483132A5072273578 @default.
- W4206483132 hasAuthorship W4206483132A5074628946 @default.
- W4206483132 hasAuthorship W4206483132A5081442629 @default.
- W4206483132 hasBestOaLocation W42064831321 @default.
- W4206483132 hasConcept C104317684 @default.
- W4206483132 hasConcept C136449434 @default.
- W4206483132 hasConcept C140704245 @default.
- W4206483132 hasConcept C150194340 @default.
- W4206483132 hasConcept C159047783 @default.
- W4206483132 hasConcept C162317418 @default.
- W4206483132 hasConcept C2522874641 @default.
- W4206483132 hasConcept C2775862345 @default.
- W4206483132 hasConcept C2776709828 @default.
- W4206483132 hasConcept C2778420691 @default.