FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206594611> ?p ?o ?g. }

• W4206594611 abstract "Abstract Carbapenem resistance and other antibiotic resistance genes (ARGs) can be found in plasmids in Acinetobacter , but many plasmid types in this genus have not been well-characterised. Here we describe the distribution, diversity and evolutionary capacity of rep group 13 (GR13) plasmids that are found in Acinetobacter species from diverse environments. Our investigation was prompted by the discovery of two GR13 plasmids in A. baumannii isolated in an intensive care unit (ICU). The plasmids harbour distinct accessory genes: pDETAB5 contains bla NDM-1 and genes that confer resistance to four further antibiotic classes, while pDETAB13 carries putative alcohol tolerance determinants. Both plasmids contain multiple dif modules, which are flanked by p dif sites recognised by XerC/XerD tyrosine recombinases. The ARG-containing dif modules in pDETAB5 are almost identical to those found in pDETAB2, a GR34 plasmid from an unrelated A. baumannii isolated in the same ICU a month prior. Examination of a further 41 complete, publicly available plasmid sequences revealed that the GR13 pangenome consists of just four core but 1086 accessory genes, 123 in the shell and 1063 in the cloud, reflecting substantial capacity for diversification. The GR13 core genome includes genes for replication and partitioning, and for a putative tyrosine recombinase. Accessory segments encode proteins with diverse putative functions, including for metabolism, antibiotic/heavy metal/alcohol tolerance, restriction-modification, an anti-phage system and multiple toxin-antitoxin systems. The movement of dif modules and actions of insertion sequences play an important role in generating diversity in GR13 plasmids. Discrete GR13 plasmid lineages are internationally disseminated and found in multiple Acinetobacter species, which suggests they are important platforms for the accumulation, horizontal transmission and persistence of accessory genes in this genus. Impact statement Acinetobacter species are particularly well-adapted for persistence in hospital environments where they pose a life-threatening infection risk to the most clinically-vulnerable patients. Plasmids with the potential to transfer multiple antibiotic resistance determinants between Acinetobacter species are therefore concerning, but most are not well-characterised. This work sheds further light on the poorly-understood mobile gene pool associated with Acinetobacter . We show here that GR13 plasmids carry a small set of core genes but have access to a highly diverse set of accessory segments that might provide fitness advantages under certain conditions. The complex evolutionary dynamics of GR13 plasmids appear to be driven by the exchange of dif modules and by the actions of a diverse population of insertion sequences. The novel dif modules characterised here emphasise the broader importance of these elements to the dissemination of accessory genes in Acinetobacter . This study has improved our understanding of the diversity and distribution of dif modules, plasmids that carry them, and how both disseminate in the continuum of Acinetobacter populations that link hospitals and the wider environment." @default.
• W4206594611 created "2022-01-25" @default.
• W4206594611 creator A5002039721 @default.
• W4206594611 creator A5021023077 @default.
• W4206594611 creator A5024002499 @default.
• W4206594611 creator A5024894491 @default.
• W4206594611 creator A5048776201 @default.
• W4206594611 creator A5065075576 @default.
• W4206594611 creator A5071285115 @default.
• W4206594611 creator A5076517379 @default.
• W4206594611 creator A5087441422 @default.
• W4206594611 creator A5087771434 @default.
• W4206594611 date "2022-01-12" @default.
• W4206594611 modified "2023-09-27" @default.
• W4206594611 title "GR13-type plasmids in <i>Acinetobacter</i> potentiate the accumulation and horizontal transfer of diverse accessory genes" @default.
• W4206594611 cites W1999397178 @default.
• W4206594611 cites W2000541324 @default.
• W4206594611 cites W2001161007 @default.
• W4206594611 cites W2023058046 @default.
• W4206594611 cites W2055380248 @default.
• W4206594611 cites W2074830661 @default.
• W4206594611 cites W2096093282 @default.
• W4206594611 cites W2105393577 @default.
• W4206594611 cites W2109089063 @default.
• W4206594611 cites W2112589522 @default.
• W4206594611 cites W2120812654 @default.
• W4206594611 cites W2125096269 @default.
• W4206594611 cites W2134852597 @default.
• W4206594611 cites W2141052558 @default.
• W4206594611 cites W2149452073 @default.
• W4206594611 cites W2153444025 @default.
• W4206594611 cites W2160161568 @default.
• W4206594611 cites W2170246082 @default.
• W4206594611 cites W2248169544 @default.
• W4206594611 cites W2396929258 @default.
• W4206594611 cites W2516480006 @default.
• W4206594611 cites W2579904633 @default.
• W4206594611 cites W2595931588 @default.
• W4206594611 cites W2612516986 @default.
• W4206594611 cites W2751583588 @default.
• W4206594611 cites W2763570542 @default.
• W4206594611 cites W2765767822 @default.
• W4206594611 cites W2793462980 @default.
• W4206594611 cites W2794298717 @default.
• W4206594611 cites W2894470898 @default.
• W4206594611 cites W2900629010 @default.
• W4206594611 cites W2936992933 @default.
• W4206594611 cites W2952167528 @default.
• W4206594611 cites W2971630607 @default.
• W4206594611 cites W2980296480 @default.
• W4206594611 cites W3028280204 @default.
• W4206594611 cites W3035204695 @default.
• W4206594611 cites W3036709412 @default.
• W4206594611 cites W3037044530 @default.
• W4206594611 cites W3045244624 @default.
• W4206594611 cites W3094987799 @default.
• W4206594611 cites W3109300655 @default.
• W4206594611 cites W3121449821 @default.
• W4206594611 cites W3126002023 @default.
• W4206594611 cites W3143063265 @default.
• W4206594611 cites W3168568464 @default.
• W4206594611 cites W3199247923 @default.
• W4206594611 cites W3207391462 @default.
• W4206594611 doi "https://doi.org/10.1101/2022.01.12.475240" @default.
• W4206594611 hasPublicationYear "2022" @default.
• W4206594611 type Work @default.
• W4206594611 citedByCount "0" @default.
• W4206594611 crossrefType "posted-content" @default.
• W4206594611 hasAuthorship W4206594611A5002039721 @default.
• W4206594611 hasAuthorship W4206594611A5021023077 @default.
• W4206594611 hasAuthorship W4206594611A5024002499 @default.
• W4206594611 hasAuthorship W4206594611A5024894491 @default.
• W4206594611 hasAuthorship W4206594611A5048776201 @default.
• W4206594611 hasAuthorship W4206594611A5065075576 @default.
• W4206594611 hasAuthorship W4206594611A5071285115 @default.
• W4206594611 hasAuthorship W4206594611A5076517379 @default.
• W4206594611 hasAuthorship W4206594611A5087441422 @default.
• W4206594611 hasAuthorship W4206594611A5087771434 @default.
• W4206594611 hasBestOaLocation W42065946111 @default.
• W4206594611 hasConcept C102414288 @default.
• W4206594611 hasConcept C104317684 @default.
• W4206594611 hasConcept C141231307 @default.
• W4206594611 hasConcept C156695909 @default.
• W4206594611 hasConcept C157887575 @default.
• W4206594611 hasConcept C22744801 @default.
• W4206594611 hasConcept C2776315533 @default.
• W4206594611 hasConcept C2777637488 @default.
• W4206594611 hasConcept C4918238 @default.
• W4206594611 hasConcept C523546767 @default.
• W4206594611 hasConcept C54355233 @default.
• W4206594611 hasConcept C86803240 @default.
• W4206594611 hasConcept C92938381 @default.
• W4206594611 hasConcept C94665300 @default.
• W4206594611 hasConceptScore W4206594611C102414288 @default.
• W4206594611 hasConceptScore W4206594611C104317684 @default.
• W4206594611 hasConceptScore W4206594611C141231307 @default.
• W4206594611 hasConceptScore W4206594611C156695909 @default.
• W4206594611 hasConceptScore W4206594611C157887575 @default.
• W4206594611 hasConceptScore W4206594611C22744801 @default.
• W4206594611 hasConceptScore W4206594611C2776315533 @default.

• next