FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206804553> ?p ?o ?g. }

• W4206804553 abstract "Sequential membrane filtration as a pre-processing step for capturing sediment-associated microorganisms could provide good quality and integrity DNA that can be preserved and kept at ambient temperatures before community profiling through culture-independent molecular techniques. However, the effects of sample pre-processing via filtration on DNA-based profiling of sediment-associated microbial community diversity and composition are poorly understood. Specifically, the influences of pre-processing on the quality and quantity of extracted DNA, high-throughput DNA sequencing reads, and detected microbial taxa need further evaluation.We assessed the impact of pre-processing freshwater sediment samples by sequential membrane filtration (from 10, 5 to 0.22 μm pore size) for 16S rRNA-based community profiling of sediment-associated microorganisms. Specifically, we examined if there would be method-driven differences between non- and pre-processed sediment samples regarding the quality and quantity of extracted DNA, PCR amplicon, resulting high-throughput sequencing reads, microbial diversity, and community composition. We found no significant difference in the qualities and quantities of extracted DNA and PCR amplicons, and the read abundance after bioinformatics processing (i.e., denoising and chimeric-read filtering steps) between the two methods. Although the non- and pre-processed sediment samples had more unique than shared amplicon sequence variants (ASVs), we report that their shared ASVs accounted for 74% of both methods' absolute read abundance. More so, at the genus level, the final collection filter identified most of the genera (95% of the reads) captured from the non-processed samples, with a total of 51 false-negative (2%) and 59 false-positive genera (3%). We demonstrate that while there were differences in shared and unique taxa, both methods revealed comparable microbial diversity and community composition.Our observations highlight the feasibility of pre-processing sediment samples for community analysis and the need to further assess sampling strategies to help conceptualize appropriate study designs for sediment-associated microbial community profiling." @default.
• W4206804553 created "2022-01-25" @default.
• W4206804553 creator A5078231275 @default.
• W4206804553 creator A5090026181 @default.
• W4206804553 date "2022-01-20" @default.
• W4206804553 modified "2023-10-18" @default.
• W4206804553 title "Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16S rRNA amplicon sequencing" @default.
• W4206804553 cites W1583408120 @default.
• W4206804553 cites W1717085993 @default.
• W4206804553 cites W1973012451 @default.
• W4206804553 cites W1979966476 @default.
• W4206804553 cites W1990480901 @default.
• W4206804553 cites W2001202072 @default.
• W4206804553 cites W2005107113 @default.
• W4206804553 cites W2011381791 @default.
• W4206804553 cites W2021541156 @default.
• W4206804553 cites W2022269454 @default.
• W4206804553 cites W2028166665 @default.
• W4206804553 cites W2031730175 @default.
• W4206804553 cites W2036897871 @default.
• W4206804553 cites W2056279562 @default.
• W4206804553 cites W2067818835 @default.
• W4206804553 cites W2073497355 @default.
• W4206804553 cites W2077276999 @default.
• W4206804553 cites W2091922579 @default.
• W4206804553 cites W2095500328 @default.
• W4206804553 cites W2106778108 @default.
• W4206804553 cites W2114392707 @default.
• W4206804553 cites W2123506747 @default.
• W4206804553 cites W2133856765 @default.
• W4206804553 cites W2143987465 @default.
• W4206804553 cites W2157402444 @default.
• W4206804553 cites W2159869703 @default.
• W4206804553 cites W2166171121 @default.
• W4206804553 cites W2172176010 @default.
• W4206804553 cites W2220064240 @default.
• W4206804553 cites W2287531489 @default.
• W4206804553 cites W2334419518 @default.
• W4206804553 cites W2335786001 @default.
• W4206804553 cites W2345813655 @default.
• W4206804553 cites W2347072353 @default.
• W4206804553 cites W2347104163 @default.
• W4206804553 cites W2369674119 @default.
• W4206804553 cites W2401404581 @default.
• W4206804553 cites W2466351767 @default.
• W4206804553 cites W2483097398 @default.
• W4206804553 cites W2571481723 @default.
• W4206804553 cites W2603618736 @default.
• W4206804553 cites W2616099261 @default.
• W4206804553 cites W2623390253 @default.
• W4206804553 cites W2739113951 @default.
• W4206804553 cites W2751013971 @default.
• W4206804553 cites W2795089968 @default.
• W4206804553 cites W2804742600 @default.
• W4206804553 cites W2810396277 @default.
• W4206804553 cites W2810960347 @default.
• W4206804553 cites W2887890414 @default.
• W4206804553 cites W2888193242 @default.
• W4206804553 cites W2897249272 @default.
• W4206804553 cites W2902924160 @default.
• W4206804553 cites W2903153560 @default.
• W4206804553 cites W2909240409 @default.
• W4206804553 cites W2914852652 @default.
• W4206804553 cites W2924106474 @default.
• W4206804553 cites W2944296834 @default.
• W4206804553 cites W2946898319 @default.
• W4206804553 cites W2963400389 @default.
• W4206804553 cites W2964298938 @default.
• W4206804553 cites W3015624719 @default.
• W4206804553 cites W3030799956 @default.
• W4206804553 cites W3043418009 @default.
• W4206804553 cites W3087512081 @default.
• W4206804553 cites W3111232965 @default.
• W4206804553 cites W3185634993 @default.
• W4206804553 cites W3200780289 @default.
• W4206804553 cites W3210852116 @default.
• W4206804553 doi "https://doi.org/10.1186/s12866-022-02441-0" @default.
• W4206804553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35057747" @default.
• W4206804553 hasPublicationYear "2022" @default.
• W4206804553 type Work @default.
• W4206804553 citedByCount "2" @default.
• W4206804553 countsByYear W42068045532022 @default.
• W4206804553 countsByYear W42068045532023 @default.
• W4206804553 crossrefType "journal-article" @default.
• W4206804553 hasAuthorship W4206804553A5078231275 @default.
• W4206804553 hasAuthorship W4206804553A5090026181 @default.
• W4206804553 hasBestOaLocation W42068045531 @default.
• W4206804553 hasConcept C104317684 @default.
• W4206804553 hasConcept C130217890 @default.
• W4206804553 hasConcept C15151743 @default.
• W4206804553 hasConcept C151730666 @default.
• W4206804553 hasConcept C18903297 @default.
• W4206804553 hasConcept C2779969263 @default.
• W4206804553 hasConcept C2816523 @default.
• W4206804553 hasConcept C2993206177 @default.
• W4206804553 hasConcept C42062724 @default.
• W4206804553 hasConcept C49105822 @default.
• W4206804553 hasConcept C523546767 @default.
• W4206804553 hasConcept C54355233 @default.
• W4206804553 hasConcept C70721500 @default.

• next