FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4206900213> ?p ?o ?g. }

• W4206900213 endingPage "i152" @default.
• W4206900213 startingPage "i151" @default.
• W4206900213 abstract "Abstract Background T resident memory (Trm) cells in the intestinal mucosa and in particular subpopulations expressing phenotypic markers such as alphaE-beta7 or NKG2D have been associated with chronic inflammatory bowel disease (IBD) activity. We hypothesize that these populations may have a direct deleterious impact on the intestinal epithelium in IBD. Methods The phenotypic study of mucosal lymphocytes was performed in two prospective cohorts: ELYP including patients with active IBD before initiation of biotherapy and REMIND including patients with ileal Crohn’s disease (CD) requiring ileocaecal resection. These analyses were performed before initiation of treatment and one year after continuous therapy in the ELYP cohort and on the resection specimen in the REMIND cohort. An innovative ex vivo autologous organoid-mucosal T cell coculture model was developed using the REMIND cohort specimens for patients and healthy ileum from individuals without IBD for controls (Figure 1). T cell infiltration within the organoid and epithelial cell death were assessed by confocal microscopy. A panel of 30 cytokines was quantified in the supernatants of cocultures. Figure 1 Results In the ELYP cohort, before the start of treatment, IBD patients had lower expression of alphaE-beta7 and NKG2D on mucosal CD8 T cells. While alphaE-beta7 and NKG2D expression on mucosal CD8 T cells had returned to normal in endoscopic responders, it remained decreased in non-responders. Similarly, the inflammatory mucosa of the REMIND surgical specimens had lower levels of CD4 and CD8 T cells expressing alphaE-beta7 and NKG2D compared with controls and non-inflamed regions. We developed a coculture model between mucosal lymphocytes and organoids generated under autologous conditions. We showed an increase in apoptotic cell death in epithelial cells from CD patients which was not found in control cocultures. There was a significant correlation between the degree of epithelial cell death and T cell infiltration (Figure 2). Various proinflammatory cytokines such as IFNgamma, TNFalpha, IL-6 and IL- 17a were also increased in the supernatants. The use of antibodies blocking the alphaE-beta7 and NKG2D pathways of interest inhibited this effect by different mechanisms (Figure 3) While anti-beta7 and anti-NKG2D had comparable effects in terms of cell death inhibition, only anti-beta7 reduced T cell infiltration. Anti-NKG2D had no effect on cell infiltration but a reduction of perforin was observed in the supernatants. Figure 2 Figure 3 CC= Coculture Conclusion These data demonstrate for the first time the direct cytotoxic deleterious effect of mucosal T cells on the epithelium of CD patients using a novel coculture model. AlphaE-beta7 and NKG2D pathways appear to be relevant in this process." @default.
• W4206900213 created "2022-01-26" @default.
• W4206900213 creator A5000888675 @default.
• W4206900213 creator A5002479356 @default.
• W4206900213 creator A5005767218 @default.
• W4206900213 creator A5005932208 @default.
• W4206900213 creator A5011868732 @default.
• W4206900213 creator A5020070531 @default.
• W4206900213 creator A5028848218 @default.
• W4206900213 creator A5032569717 @default.
• W4206900213 creator A5034877186 @default.
• W4206900213 creator A5038818440 @default.
• W4206900213 creator A5044768831 @default.
• W4206900213 creator A5046316577 @default.
• W4206900213 creator A5046775079 @default.
• W4206900213 creator A5047815138 @default.
• W4206900213 creator A5049289749 @default.
• W4206900213 creator A5059539924 @default.
• W4206900213 creator A5063024699 @default.
• W4206900213 creator A5078977008 @default.
• W4206900213 creator A5080213094 @default.
• W4206900213 creator A5080883164 @default.
• W4206900213 creator A5082371267 @default.
• W4206900213 creator A5083648728 @default.
• W4206900213 creator A5089394297 @default.
• W4206900213 date "2022-01-01" @default.
• W4206900213 modified "2023-09-27" @default.
• W4206900213 title "P031 Intestinal lymphoepithelial interactions in Crohn’s disease: modeling alphaE-beta7 and NKG2D blockade" @default.
• W4206900213 doi "https://doi.org/10.1093/ecco-jcc/jjab232.160" @default.
• W4206900213 hasPublicationYear "2022" @default.
• W4206900213 type Work @default.
• W4206900213 citedByCount "0" @default.
• W4206900213 crossrefType "journal-article" @default.
• W4206900213 hasAuthorship W4206900213A5000888675 @default.
• W4206900213 hasAuthorship W4206900213A5002479356 @default.
• W4206900213 hasAuthorship W4206900213A5005767218 @default.
• W4206900213 hasAuthorship W4206900213A5005932208 @default.
• W4206900213 hasAuthorship W4206900213A5011868732 @default.
• W4206900213 hasAuthorship W4206900213A5020070531 @default.
• W4206900213 hasAuthorship W4206900213A5028848218 @default.
• W4206900213 hasAuthorship W4206900213A5032569717 @default.
• W4206900213 hasAuthorship W4206900213A5034877186 @default.
• W4206900213 hasAuthorship W4206900213A5038818440 @default.
• W4206900213 hasAuthorship W4206900213A5044768831 @default.
• W4206900213 hasAuthorship W4206900213A5046316577 @default.
• W4206900213 hasAuthorship W4206900213A5046775079 @default.
• W4206900213 hasAuthorship W4206900213A5047815138 @default.
• W4206900213 hasAuthorship W4206900213A5049289749 @default.
• W4206900213 hasAuthorship W4206900213A5059539924 @default.
• W4206900213 hasAuthorship W4206900213A5063024699 @default.
• W4206900213 hasAuthorship W4206900213A5078977008 @default.
• W4206900213 hasAuthorship W4206900213A5080213094 @default.
• W4206900213 hasAuthorship W4206900213A5080883164 @default.
• W4206900213 hasAuthorship W4206900213A5082371267 @default.
• W4206900213 hasAuthorship W4206900213A5083648728 @default.
• W4206900213 hasAuthorship W4206900213A5089394297 @default.
• W4206900213 hasBestOaLocation W42069002131 @default.
• W4206900213 hasConcept C126322002 @default.
• W4206900213 hasConcept C150903083 @default.
• W4206900213 hasConcept C154317977 @default.
• W4206900213 hasConcept C167672396 @default.
• W4206900213 hasConcept C202751555 @default.
• W4206900213 hasConcept C203014093 @default.
• W4206900213 hasConcept C207001950 @default.
• W4206900213 hasConcept C26291073 @default.
• W4206900213 hasConcept C2776090121 @default.
• W4206900213 hasConcept C2778260677 @default.
• W4206900213 hasConcept C2778867473 @default.
• W4206900213 hasConcept C2779134260 @default.
• W4206900213 hasConcept C3019368612 @default.
• W4206900213 hasConcept C502942594 @default.
• W4206900213 hasConcept C55493867 @default.
• W4206900213 hasConcept C71924100 @default.
• W4206900213 hasConcept C86803240 @default.
• W4206900213 hasConcept C8891405 @default.
• W4206900213 hasConcept C90924648 @default.
• W4206900213 hasConceptScore W4206900213C126322002 @default.
• W4206900213 hasConceptScore W4206900213C150903083 @default.
• W4206900213 hasConceptScore W4206900213C154317977 @default.
• W4206900213 hasConceptScore W4206900213C167672396 @default.
• W4206900213 hasConceptScore W4206900213C202751555 @default.
• W4206900213 hasConceptScore W4206900213C203014093 @default.
• W4206900213 hasConceptScore W4206900213C207001950 @default.
• W4206900213 hasConceptScore W4206900213C26291073 @default.
• W4206900213 hasConceptScore W4206900213C2776090121 @default.
• W4206900213 hasConceptScore W4206900213C2778260677 @default.
• W4206900213 hasConceptScore W4206900213C2778867473 @default.
• W4206900213 hasConceptScore W4206900213C2779134260 @default.
• W4206900213 hasConceptScore W4206900213C3019368612 @default.
• W4206900213 hasConceptScore W4206900213C502942594 @default.
• W4206900213 hasConceptScore W4206900213C55493867 @default.
• W4206900213 hasConceptScore W4206900213C71924100 @default.
• W4206900213 hasConceptScore W4206900213C86803240 @default.
• W4206900213 hasConceptScore W4206900213C8891405 @default.
• W4206900213 hasConceptScore W4206900213C90924648 @default.
• W4206900213 hasIssue "Supplement_1" @default.
• W4206900213 hasLocation W42069002131 @default.
• W4206900213 hasOpenAccess W4206900213 @default.

• next