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- W4206969968 abstract "Marchantia polymorpha is a model liverwort and its overall low genetic redundancy is advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools in recent years. However, interactomics studies applying proximity labelling are currently limited to angiosperm species in plants. Here, we established and evaluated a miniTurbo-based interactomics method in M. polymorpha using MpSYP12A and MpSYP13B, two plasma membrane-localized SNARE proteins, as baits. We show that our method yields a manifold of potential interactors of MpSYP12A and MpSYP13B compared to a co-immunoprecipitation approach. Our method could capture specific candidates for each SNARE. We conclude that a miniTurbo-based method is a feasible tool for interactomics in M. polymorpha and potentially applicable to other model bryophytes. Our interactome dataset on MpSYP12A and MpSYP13B will be a useful resource to elucidate the evolution of SNARE functions." @default.
- W4206969968 created "2022-01-26" @default.
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- W4206969968 date "2022-01-25" @default.
- W4206969968 modified "2023-10-18" @default.
- W4206969968 title "miniTurbo-based interactomics of two plasma membrane-localized SNARE proteins in Marchantia polymorpha" @default.
- W4206969968 doi "https://doi.org/10.1101/2022.01.21.477208" @default.
- W4206969968 hasPublicationYear "2022" @default.
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