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- W4206977963 abstract "Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media." @default.
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- W4206977963 date "2021-12-23" @default.
- W4206977963 modified "2023-10-16" @default.
- W4206977963 title "Study on the Plasmid and Genomic DNA Stability of DH5α Host System" @default.
- W4206977963 doi "https://doi.org/10.9734/jpri/2021/v33i60b34751" @default.
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