FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4207014718> ?p ?o ?g. }

• W4207014718 endingPage "103825" @default.
• W4207014718 startingPage "103825" @default.
• W4207014718 abstract "BackgroundRheumatoid arthritis (RA) is an inflammatory disease that manifests as a preclinical stage of systemic autoimmunity followed by chronic progressive synovitis. Disease-associated genetic SNP variants predominantly map to non-coding, regulatory regions of functional importance in CD4 T cells, implicating these cells as key regulators. A better understanding of the epigenome of CD4 T cells holds the promise of providing information on the interaction between genetic susceptibility and exogenous factors.MethodsWe mapped regions of chromatin accessibility using ATAC-seq in peripheral CD4 T cell subsets of patients with RA (n=18) and compared them to T cells from patients with psoriatic arthritis (n=11) and age-matched healthy controls (n=10). Transcripts of selected genes were quantified using qPCR.FindingsRA-associated epigenetic signatures were identified that in part overlapped between central and effector memory CD4 T cells and that were to a lesser extent already present in naïve cells. Sites more accessible in RA were highly enriched for the motif of the transcription factor (TF) CTCF suggesting differences in the three-dimensional chromatin structure. Unexpectedly, sites with reduced chromatin accessibility were enriched for motifs of TFs pertinent for T cell function. Most strikingly, super-enhancers encompassing RA-associated SNPs were less accessible. Analysis of selected transcripts and published DNA methylation patterns were consistent with this finding. The preferential loss in accessibility at these super-enhancers was seen in patients with high and low disease activity and on a variety of immunosuppressive treatment modalities.InterpretationDisease-associated genes are epigenetically less poised to respond in CD4 T cells from patients with established RA.FundingThis work was supported by I01 BX001669 from the Veterans Administration. Rheumatoid arthritis (RA) is an inflammatory disease that manifests as a preclinical stage of systemic autoimmunity followed by chronic progressive synovitis. Disease-associated genetic SNP variants predominantly map to non-coding, regulatory regions of functional importance in CD4 T cells, implicating these cells as key regulators. A better understanding of the epigenome of CD4 T cells holds the promise of providing information on the interaction between genetic susceptibility and exogenous factors. We mapped regions of chromatin accessibility using ATAC-seq in peripheral CD4 T cell subsets of patients with RA (n=18) and compared them to T cells from patients with psoriatic arthritis (n=11) and age-matched healthy controls (n=10). Transcripts of selected genes were quantified using qPCR. RA-associated epigenetic signatures were identified that in part overlapped between central and effector memory CD4 T cells and that were to a lesser extent already present in naïve cells. Sites more accessible in RA were highly enriched for the motif of the transcription factor (TF) CTCF suggesting differences in the three-dimensional chromatin structure. Unexpectedly, sites with reduced chromatin accessibility were enriched for motifs of TFs pertinent for T cell function. Most strikingly, super-enhancers encompassing RA-associated SNPs were less accessible. Analysis of selected transcripts and published DNA methylation patterns were consistent with this finding. The preferential loss in accessibility at these super-enhancers was seen in patients with high and low disease activity and on a variety of immunosuppressive treatment modalities. Disease-associated genes are epigenetically less poised to respond in CD4 T cells from patients with established RA." @default.
• W4207014718 created "2022-01-26" @default.
• W4207014718 creator A5006681271 @default.
• W4207014718 creator A5012971026 @default.
• W4207014718 creator A5030518549 @default.
• W4207014718 creator A5054799649 @default.
• W4207014718 creator A5056587089 @default.
• W4207014718 creator A5058280095 @default.
• W4207014718 creator A5066136174 @default.
• W4207014718 creator A5082337580 @default.
• W4207014718 date "2022-02-01" @default.
• W4207014718 modified "2023-10-09" @default.
• W4207014718 title "Reduced chromatin accessibility to CD4 T cell super-enhancers encompassing susceptibility loci of rheumatoid arthritis" @default.
• W4207014718 cites W1964598084 @default.
• W4207014718 cites W1972298341 @default.
• W4207014718 cites W1973639800 @default.
• W4207014718 cites W1976951740 @default.
• W4207014718 cites W1979594866 @default.
• W4207014718 cites W1982865105 @default.
• W4207014718 cites W1989590412 @default.
• W4207014718 cites W1994009027 @default.
• W4207014718 cites W1995745687 @default.
• W4207014718 cites W2004966039 @default.
• W4207014718 cites W2014677321 @default.
• W4207014718 cites W2019446509 @default.
• W4207014718 cites W2023047365 @default.
• W4207014718 cites W2035618305 @default.
• W4207014718 cites W2084317828 @default.
• W4207014718 cites W2103393606 @default.
• W4207014718 cites W2112094316 @default.
• W4207014718 cites W2122624605 @default.
• W4207014718 cites W2140073887 @default.
• W4207014718 cites W2146512944 @default.
• W4207014718 cites W2146994504 @default.
• W4207014718 cites W2152014155 @default.
• W4207014718 cites W2170219300 @default.
• W4207014718 cites W2170830174 @default.
• W4207014718 cites W2309229101 @default.
• W4207014718 cites W2530386703 @default.
• W4207014718 cites W2530760657 @default.
• W4207014718 cites W2545009331 @default.
• W4207014718 cites W2587865057 @default.
• W4207014718 cites W2589710212 @default.
• W4207014718 cites W2600679322 @default.
• W4207014718 cites W2738294709 @default.
• W4207014718 cites W2777803696 @default.
• W4207014718 cites W2785406156 @default.
• W4207014718 cites W2787981407 @default.
• W4207014718 cites W2903018958 @default.
• W4207014718 cites W2912285780 @default.
• W4207014718 cites W2950341312 @default.
• W4207014718 cites W2952815301 @default.
• W4207014718 cites W2960850010 @default.
• W4207014718 cites W2998546112 @default.
• W4207014718 cites W2998970866 @default.
• W4207014718 cites W3000874222 @default.
• W4207014718 cites W3006394248 @default.
• W4207014718 cites W3008136382 @default.
• W4207014718 cites W3014240243 @default.
• W4207014718 cites W3018856393 @default.
• W4207014718 cites W3047528414 @default.
• W4207014718 cites W3082153767 @default.
• W4207014718 cites W3097998438 @default.
• W4207014718 cites W3109478647 @default.
• W4207014718 cites W3109820229 @default.
• W4207014718 cites W3110487516 @default.
• W4207014718 cites W3112579814 @default.
• W4207014718 cites W3120929304 @default.
• W4207014718 cites W3126733217 @default.
• W4207014718 cites W3127935100 @default.
• W4207014718 cites W3216944928 @default.
• W4207014718 doi "https://doi.org/10.1016/j.ebiom.2022.103825" @default.
• W4207014718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35085847" @default.
• W4207014718 hasPublicationYear "2022" @default.
• W4207014718 type Work @default.
• W4207014718 citedByCount "0" @default.
• W4207014718 crossrefType "journal-article" @default.
• W4207014718 hasAuthorship W4207014718A5006681271 @default.
• W4207014718 hasAuthorship W4207014718A5012971026 @default.
• W4207014718 hasAuthorship W4207014718A5030518549 @default.
• W4207014718 hasAuthorship W4207014718A5054799649 @default.
• W4207014718 hasAuthorship W4207014718A5056587089 @default.
• W4207014718 hasAuthorship W4207014718A5058280095 @default.
• W4207014718 hasAuthorship W4207014718A5066136174 @default.
• W4207014718 hasAuthorship W4207014718A5082337580 @default.
• W4207014718 hasBestOaLocation W42070147183 @default.
• W4207014718 hasConcept C104317684 @default.
• W4207014718 hasConcept C106208931 @default.
• W4207014718 hasConcept C111936080 @default.
• W4207014718 hasConcept C117717151 @default.
• W4207014718 hasConcept C135763542 @default.
• W4207014718 hasConcept C150194340 @default.
• W4207014718 hasConcept C153209595 @default.
• W4207014718 hasConcept C190727270 @default.
• W4207014718 hasConcept C2778760011 @default.
• W4207014718 hasConcept C41091548 @default.
• W4207014718 hasConcept C54355233 @default.
• W4207014718 hasConcept C83640560 @default.

• next