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- W4207024642 abstract "Porphyrins are among the first ligands that have been tested for their quadruplex binding and stabilization potential. We report the differential interaction of the positional cationic porphyrin isomers TMPyP3 and TMPyP4 with a parallel G-quadruplex (GQ) formed by 33-mer (TP) regulatory sequence present in the promoter region of the human multidrug resistance protein 1 (MRP1) transporter gene. This GQ element encompasses the three evolutionary conserved SP1 transcription factor binding sites. Taking into account that SP1 binds to a non-canonical GQ motif with higher affinity than to a canonical duplex DNA consensus motif, it is suggestive that GQ distortion by cationic porphyrin will have important implications in the regulation of MRP1 expression. Herein, we employed biophysical analysis using circular dichroism, visible absorption, UV-thermal melting and steady-state fluorescence spectroscopy, reporting destabilization of MRP1 GQ by cationic porphyrins. Results suggest that TMPyP4 and TMPyP3 interact with GQ with a binding affinity of 106 to 107 M-1 . Thermodynamic analysis indicated a significant decrease in melting temperature of GQ (ΔTm of 15.5°C-23.5°C), in the presence of 2 times excess of porphyrins. This study provides the biophysical evidence indicating the destabilisation of a parallel DNA G-quadruplex by cationic porphyrins." @default.
- W4207024642 created "2022-01-26" @default.
- W4207024642 creator A5025811477 @default.
- W4207024642 creator A5070033249 @default.
- W4207024642 creator A5084752151 @default.
- W4207024642 date "2022-01-06" @default.
- W4207024642 modified "2023-10-15" @default.
- W4207024642 title "Porphyrin induced structural destabilization of a parallel DNA G‐quadruplex in human <i>MRP1</i> gene promoter" @default.
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- W4207024642 doi "https://doi.org/10.1002/jmr.2950" @default.
- W4207024642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34990028" @default.
- W4207024642 hasPublicationYear "2022" @default.
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