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- W4207045013 abstract "Carbohydrate-specific antibodies can serve as valuable tools to monitor alterations in the extracellular matrix resulting from pathologies. Here, the keratan sulfate-specific monoclonal antibody MZ15 was characterized in more detail by immunofluorescence microscopy as well as laser ablation ICP-MS using tissue cryosections and paraffin-embedded samples. Pretreatment with keratanase II prevented staining of samples and therefore demonstrated efficient enzymatic keratan sulfate degradation. Random fluorescent labeling and site-directed introduction of a metal cage into MZ15 were successful and allowed for a highly sensitive detection of the keratan sulfate landscape in the corneal stroma from rats and human tissue." @default.
- W4207045013 created "2022-01-26" @default.
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- W4207045013 date "2022-01-25" @default.
- W4207045013 modified "2023-10-15" @default.
- W4207045013 title "Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS" @default.
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- W4207045013 doi "https://doi.org/10.1021/acsabm.1c01240" @default.
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