Matches in SemOpenAlex for { <https://semopenalex.org/work/W4207045789> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4207045789 endingPage "558" @default.
- W4207045789 startingPage "550" @default.
- W4207045789 abstract "We have recently shown that SmbP, the small metal-binding protein of Nitrosomonas europaea, can be employed as a fusion protein to express and purify recombinant proteins and peptides in Escherichia coli. SmbP increases solubility, allows simple, one-step purification through affinity chromatography, and provides superior final yields due to its low molecular weight. In this work, we report for the first time the use of SmbP to produce a recombinant peptide with anticancer activity: the antitumor-analgesic peptide (BmK-AGAP), a neurotoxin isolated from the venom of the Chinese scorpion Buthus martensii Karsch. This peptide was expressed in Escherichia coli SHuffle for correct, cytoplasmic, disulfide bond formation and tagged with SmbP at the N-terminus to improve its solubility and allow purification using immobilized metal affinity chromatography. SmbP_BmK-AGAP was found in the soluble fraction of the cell lysate. After purification and removal of SmbP by digestion with enterokinase, 1.8 mg of pure and highly active rBmK-AGAP was obtained per liter of cell culture. rBmK-AGAP exhibited antiproliferative activity on the MCF-7 cancer cell line, with a half-maximal inhibitory concentration value of 7.24 μM. Based on these results, we considered SmbP to be a suitable carrier protein for the production of recombinant, biologically active BmK-AGAP. We propose that SmbP should be an attractive fusion protein for the expression and purification of additional recombinant proteins or peptides that display anticancer activities." @default.
- W4207045789 created "2022-01-26" @default.
- W4207045789 creator A5006217735 @default.
- W4207045789 creator A5015777955 @default.
- W4207045789 creator A5020723563 @default.
- W4207045789 creator A5060610479 @default.
- W4207045789 creator A5068795096 @default.
- W4207045789 creator A5072563309 @default.
- W4207045789 date "2022-01-22" @default.
- W4207045789 modified "2023-10-14" @default.
- W4207045789 title "The Small Metal-Binding Protein SmbP Improves the Expression and Purification of the Recombinant Antitumor-Analgesic Peptide from the Chinese Scorpion Buthus martensii Karsch in Escherichia coli" @default.
- W4207045789 cites W1842363969 @default.
- W4207045789 cites W1967636335 @default.
- W4207045789 cites W1972456229 @default.
- W4207045789 cites W1983011326 @default.
- W4207045789 cites W1992546539 @default.
- W4207045789 cites W2063736381 @default.
- W4207045789 cites W2080967293 @default.
- W4207045789 cites W2105300484 @default.
- W4207045789 cites W2135931671 @default.
- W4207045789 cites W2145981136 @default.
- W4207045789 cites W2472312029 @default.
- W4207045789 cites W2518788627 @default.
- W4207045789 cites W2567569673 @default.
- W4207045789 cites W2598922467 @default.
- W4207045789 cites W2806832729 @default.
- W4207045789 cites W2913659774 @default.
- W4207045789 cites W2921873665 @default.
- W4207045789 cites W2937667886 @default.
- W4207045789 cites W2999417355 @default.
- W4207045789 cites W3006475516 @default.
- W4207045789 cites W3033421897 @default.
- W4207045789 cites W3095874924 @default.
- W4207045789 cites W3096081800 @default.
- W4207045789 cites W3097742030 @default.
- W4207045789 cites W3207699057 @default.
- W4207045789 doi "https://doi.org/10.3390/cimb44020038" @default.
- W4207045789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35723324" @default.
- W4207045789 hasPublicationYear "2022" @default.
- W4207045789 type Work @default.
- W4207045789 citedByCount "0" @default.
- W4207045789 crossrefType "journal-article" @default.
- W4207045789 hasAuthorship W4207045789A5006217735 @default.
- W4207045789 hasAuthorship W4207045789A5015777955 @default.
- W4207045789 hasAuthorship W4207045789A5020723563 @default.
- W4207045789 hasAuthorship W4207045789A5060610479 @default.
- W4207045789 hasAuthorship W4207045789A5068795096 @default.
- W4207045789 hasAuthorship W4207045789A5072563309 @default.
- W4207045789 hasBestOaLocation W42070457891 @default.
- W4207045789 hasConcept C104317684 @default.
- W4207045789 hasConcept C123894998 @default.
- W4207045789 hasConcept C153911025 @default.
- W4207045789 hasConcept C179247698 @default.
- W4207045789 hasConcept C181199279 @default.
- W4207045789 hasConcept C185592680 @default.
- W4207045789 hasConcept C2779281246 @default.
- W4207045789 hasConcept C40767141 @default.
- W4207045789 hasConcept C547475151 @default.
- W4207045789 hasConcept C55493867 @default.
- W4207045789 hasConcept C86803240 @default.
- W4207045789 hasConcept C8985478 @default.
- W4207045789 hasConceptScore W4207045789C104317684 @default.
- W4207045789 hasConceptScore W4207045789C123894998 @default.
- W4207045789 hasConceptScore W4207045789C153911025 @default.
- W4207045789 hasConceptScore W4207045789C179247698 @default.
- W4207045789 hasConceptScore W4207045789C181199279 @default.
- W4207045789 hasConceptScore W4207045789C185592680 @default.
- W4207045789 hasConceptScore W4207045789C2779281246 @default.
- W4207045789 hasConceptScore W4207045789C40767141 @default.
- W4207045789 hasConceptScore W4207045789C547475151 @default.
- W4207045789 hasConceptScore W4207045789C55493867 @default.
- W4207045789 hasConceptScore W4207045789C86803240 @default.
- W4207045789 hasConceptScore W4207045789C8985478 @default.
- W4207045789 hasFunder F4320322803 @default.
- W4207045789 hasIssue "2" @default.
- W4207045789 hasLocation W42070457891 @default.
- W4207045789 hasLocation W42070457892 @default.
- W4207045789 hasLocation W42070457893 @default.
- W4207045789 hasLocation W42070457894 @default.
- W4207045789 hasOpenAccess W4207045789 @default.
- W4207045789 hasPrimaryLocation W42070457891 @default.
- W4207045789 hasRelatedWork W2009554101 @default.
- W4207045789 hasRelatedWork W2168128140 @default.
- W4207045789 hasRelatedWork W2353441596 @default.
- W4207045789 hasRelatedWork W2357972350 @default.
- W4207045789 hasRelatedWork W2361670818 @default.
- W4207045789 hasRelatedWork W2371993564 @default.
- W4207045789 hasRelatedWork W2372506654 @default.
- W4207045789 hasRelatedWork W2381845779 @default.
- W4207045789 hasRelatedWork W3032212195 @default.
- W4207045789 hasRelatedWork W3149679323 @default.
- W4207045789 hasVolume "44" @default.
- W4207045789 isParatext "false" @default.
- W4207045789 isRetracted "false" @default.
- W4207045789 workType "article" @default.