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- W4207047849 abstract "Abstract Corneal limbal stem cell (LSC) transplantation is a standard approach to treating corneal epithelial diseases. Scaffolds such as human amniotic membrane (hAM) are commonly employed for the in vitro culture and as a carrier for in vivo transplantation. However, they carry the risk of biological contamination. We have earlier reported a thermoreversible gelation polymer (TGP) scaffold to support the in vitro culture of human LSC and the transplantation in a rabbit animal model. We herein report the capabilities of the TGP scaffold to serve as an encapsulation support during transplantation and to enable engraftment for corneal regeneration. The results showed that TGP allowed human-donor cornea-derived LSCs to proliferate well in vitro, compared to hAM and the cells encapsulated in TGP and transplanted in vitro onto a human cadaver donor cornea denuded of its epithelium, migrated on the ocular surface to cover it almost totally, and proliferated to form a continuous layer in 25 days. Immunofluorescence staining of TGP-cultured cells was positive for LSC markers (p63, ABCG2, Connexin 43 and Integrin β), proving that the TGP helps to preserve the limbal cells’ stemness. TGP is found to be a multipurpose scaffold for (i) in vitro culture, (ii) in vivo encapsulation, and transplantation (iii), enabling engraftment of LSCs in this study, with potentials to extend its application in cell-based therapies in several regenerative medicine approaches." @default.
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- W4207047849 date "2022-01-25" @default.
- W4207047849 modified "2023-10-16" @default.
- W4207047849 title "Engraftment and proliferation of thermoreversible-gelation-polymer-encapsulated human corneal limbal-stem-cells on ocular surface of a cadaver cornea" @default.
- W4207047849 doi "https://doi.org/10.21203/rs.3.rs-1285391/v1" @default.
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