Matches in SemOpenAlex for { <https://semopenalex.org/work/W4207050975> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W4207050975 abstract "Cancer may be initiated by covalent modification of DNA by genotoxic molecules coming from diet, environment, inflammation, and other sources. For most of these genotoxicants there is little evidence of their identity. DNA adductomics is a new research field, aiming to screen for unknown DNA adducts by high resolution mass spectrometry (HRMS). However, due to the low abundance of DNA adducts, DNA adductomics presents several analytical challenges. In this work, a sensitive untargeted DNA adductomics method was developed by using ultra-high performance liquid chromatography (UHPLC) coupled via an electrospray ionization source (ESI) to a quadrupole-time of flight MS instrumentation. Mobile phases with ammonium bicarbonate gave the best signal enhancement. The MS capillary voltage, cone voltage and detector voltage had most effect on the response of the DNA adducts. A low adsorption vial was selected for reducing analyte loss. A hybrid surface coated HSST3 premier column was tested for reducing adsorption of the DNA adducts. The optimized method was applied to analyse DNA adducts in calf thymus and cat colon DNA by performing a MSE acquisition and screening for loss of deoxyribose, both in-source and in the fragmentation spectra, and for the nucleobase fragment ions. The putative DNA adducts were matched with an in-house DNA adduct database. Thirteen DNA adducts were observed in DNA from calf thymus and cat colon, four of them never reported before, showing promise for the application of this untargeted method in future human studies." @default.
- W4207050975 created "2022-01-26" @default.
- W4207050975 creator A5035166803 @default.
- W4207050975 creator A5068820590 @default.
- W4207050975 date "2022-01-24" @default.
- W4207050975 modified "2023-09-30" @default.
- W4207050975 title "Development of an untargeted DNA adductomics method by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry" @default.
- W4207050975 doi "https://doi.org/10.33774/chemrxiv-2021-pvr0m-v3" @default.
- W4207050975 hasPublicationYear "2022" @default.
- W4207050975 type Work @default.
- W4207050975 citedByCount "0" @default.
- W4207050975 crossrefType "posted-content" @default.
- W4207050975 hasAuthorship W4207050975A5035166803 @default.
- W4207050975 hasAuthorship W4207050975A5068820590 @default.
- W4207050975 hasBestOaLocation W42070509751 @default.
- W4207050975 hasConcept C10390740 @default.
- W4207050975 hasConcept C108204754 @default.
- W4207050975 hasConcept C143425029 @default.
- W4207050975 hasConcept C162356407 @default.
- W4207050975 hasConcept C178790620 @default.
- W4207050975 hasConcept C185592680 @default.
- W4207050975 hasConcept C190283241 @default.
- W4207050975 hasConcept C2777709666 @default.
- W4207050975 hasConcept C2781247443 @default.
- W4207050975 hasConcept C31573885 @default.
- W4207050975 hasConcept C40684141 @default.
- W4207050975 hasConcept C43617362 @default.
- W4207050975 hasConcept C43759708 @default.
- W4207050975 hasConcept C552990157 @default.
- W4207050975 hasConcept C55493867 @default.
- W4207050975 hasConcept C61234307 @default.
- W4207050975 hasConceptScore W4207050975C10390740 @default.
- W4207050975 hasConceptScore W4207050975C108204754 @default.
- W4207050975 hasConceptScore W4207050975C143425029 @default.
- W4207050975 hasConceptScore W4207050975C162356407 @default.
- W4207050975 hasConceptScore W4207050975C178790620 @default.
- W4207050975 hasConceptScore W4207050975C185592680 @default.
- W4207050975 hasConceptScore W4207050975C190283241 @default.
- W4207050975 hasConceptScore W4207050975C2777709666 @default.
- W4207050975 hasConceptScore W4207050975C2781247443 @default.
- W4207050975 hasConceptScore W4207050975C31573885 @default.
- W4207050975 hasConceptScore W4207050975C40684141 @default.
- W4207050975 hasConceptScore W4207050975C43617362 @default.
- W4207050975 hasConceptScore W4207050975C43759708 @default.
- W4207050975 hasConceptScore W4207050975C552990157 @default.
- W4207050975 hasConceptScore W4207050975C55493867 @default.
- W4207050975 hasConceptScore W4207050975C61234307 @default.
- W4207050975 hasLocation W42070509751 @default.
- W4207050975 hasOpenAccess W4207050975 @default.
- W4207050975 hasPrimaryLocation W42070509751 @default.
- W4207050975 hasRelatedWork W1609875 @default.
- W4207050975 hasRelatedWork W176074 @default.
- W4207050975 hasRelatedWork W2243583 @default.
- W4207050975 hasRelatedWork W2339581 @default.
- W4207050975 hasRelatedWork W3196370 @default.
- W4207050975 hasRelatedWork W4362161 @default.
- W4207050975 hasRelatedWork W4452979 @default.
- W4207050975 hasRelatedWork W45987493 @default.
- W4207050975 hasRelatedWork W49709537 @default.
- W4207050975 hasRelatedWork W6727018 @default.
- W4207050975 isParatext "false" @default.
- W4207050975 isRetracted "false" @default.
- W4207050975 workType "article" @default.