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- W4207058499 abstract "Abstract By offering extremely long contiguous characterization of individual DNA molecules, rapidly emerging long‐read sequencing strategies offer comprehensive insights into the organization of genetic information in genomes and metagenomes. However, successful long‐read sequencing experiments demand high concentrations of highly purified DNA of high molecular weight (HMW), which limits the utility of established DNA extraction kits designed for short‐read sequencing. The challenges associated with input DNA quality intensify further when working with complex environmental samples of low microbial biomass, which requires new protocols that are tailored to study metagenomes with long‐read sequencing. Here, we use human tongue scrapings to benchmark six HMW DNA extraction strategies that are based on commercially available kits, phenol–chloroform (PC) extraction and agarose encasement followed by agarase digestion. A typical end goal of HMW DNA extractions is to obtain the longest possible reads during sequencing, which is often achieved by PC extractions, as demonstrated in sequencing of cultured cells. Yet our analyses that consider overall read‐size distribution, assembly performance and the number of circularized elements found in sequencing results suggest that column‐based kits with enzyme supplementation, rather than PC methods, may be more appropriate for long‐read sequencing of metagenomes." @default.
- W4207058499 created "2022-01-26" @default.
- W4207058499 creator A5009016291 @default.
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- W4207058499 date "2022-02-07" @default.
- W4207058499 modified "2023-10-14" @default.
- W4207058499 title "High molecular weight DNA extraction strategies for long‐read sequencing of complex metagenomes" @default.
- W4207058499 cites W1519765314 @default.
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- W4207058499 cites W1982855075 @default.
- W4207058499 cites W2034285706 @default.
- W4207058499 cites W2079520182 @default.
- W4207058499 cites W2079758378 @default.
- W4207058499 cites W2090110425 @default.
- W4207058499 cites W2100499274 @default.
- W4207058499 cites W2108234281 @default.
- W4207058499 cites W2110417468 @default.
- W4207058499 cites W2117522399 @default.
- W4207058499 cites W2119012678 @default.
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- W4207058499 cites W2124637227 @default.
- W4207058499 cites W2127175247 @default.
- W4207058499 cites W2127407003 @default.
- W4207058499 cites W2128769815 @default.
- W4207058499 cites W2135293770 @default.
- W4207058499 cites W2141920662 @default.
- W4207058499 cites W2143485490 @default.
- W4207058499 cites W2149118757 @default.
- W4207058499 cites W2159406441 @default.
- W4207058499 cites W2162055898 @default.
- W4207058499 cites W2170551349 @default.
- W4207058499 cites W2194172909 @default.
- W4207058499 cites W2252298505 @default.
- W4207058499 cites W2274912893 @default.
- W4207058499 cites W2315521535 @default.
- W4207058499 cites W2401404581 @default.
- W4207058499 cites W2469764418 @default.
- W4207058499 cites W2513506562 @default.
- W4207058499 cites W2575750030 @default.
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- W4207058499 cites W2599417231 @default.
- W4207058499 cites W2742647527 @default.
- W4207058499 cites W2789843538 @default.
- W4207058499 cites W2794660864 @default.
- W4207058499 cites W2805238404 @default.
- W4207058499 cites W2886336530 @default.
- W4207058499 cites W2893909145 @default.
- W4207058499 cites W2908149669 @default.
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- W4207058499 cites W2918965813 @default.
- W4207058499 cites W2919922919 @default.
- W4207058499 cites W2940459311 @default.
- W4207058499 cites W2948263509 @default.
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- W4207058499 cites W2950671516 @default.
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- W4207058499 cites W2999199337 @default.
- W4207058499 cites W3005528502 @default.
- W4207058499 cites W3006022816 @default.
- W4207058499 cites W3008800330 @default.
- W4207058499 cites W3009249371 @default.
- W4207058499 cites W3011678041 @default.
- W4207058499 cites W3013031207 @default.
- W4207058499 cites W3014441729 @default.
- W4207058499 cites W3019706413 @default.
- W4207058499 cites W3021482777 @default.
- W4207058499 cites W3081166727 @default.
- W4207058499 cites W3087086484 @default.
- W4207058499 cites W3091506611 @default.
- W4207058499 cites W3102430011 @default.
- W4207058499 cites W3108447880 @default.
- W4207058499 cites W3109164186 @default.
- W4207058499 cites W3112234114 @default.
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- W4207058499 cites W3119314116 @default.
- W4207058499 cites W3120395810 @default.
- W4207058499 cites W3141597806 @default.
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- W4207058499 doi "https://doi.org/10.1111/1755-0998.13588" @default.
- W4207058499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35068060" @default.
- W4207058499 hasPublicationYear "2022" @default.
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