Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210349767> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W4210349767 endingPage "lxi" @default.
- W4210349767 startingPage "lxi" @default.
- W4210349767 abstract "The U1, U2, U4/U6, and U5 small nuclear ribonucleoproteins (snRNPs) are subunits of splicing complexes that remove introns from mRNA precursors. snRNPs show a complex, transcription-dependent localization pattern in the nucleoplasm of mammalian cells that results from their association with several distinct subnuclear structures, including interchromatin granule clusters, perichromatin fibrils, and coiled bodies. Here we report the analysis of snRNP localization and interaction with the coiled body in live human cells using fusions of snRNP proteins and p80 coilin to the Green Fluorescent Protein (GFP). Despite the large size of the GFP tag, GFP fusions to both the core snRNP SmE and U1 specific U1A proteins assemble into snRNP particles and give an identical nuclear localization pattern to their endogenous counterparts. GFP-coilin localizes specifically to coiled bodies in a transcription-dependent fashion and provides an accurate marker for coiled bodies in a variety of human cell lines. Treatment of cells with the selective ser/thr-protein phosphatase inhibitor, okadaic acid, causes both GFP-snRNP and GFP-coilin proteins to accumulate within nucleoli, but does not result in nucleolar accumulation of the GFP-fused non-snRNP protein splicing factor ASF/SF2. In all four human cell lines tested, expression of a GFP-fused p80 coilin mutant with a single serine to aspartate substitution also caused nucleolar accumulation of splicing snRNPs and coilin, but not ASF/SF2, in structures resembling coiled bodies when viewed by electron microscopy. This work establishes an experimental system for analyzing snRNP trafficking in living cells and provides evidence that a reversible protein phosphorylation mechanism is involved in regulating interaction of snRNPs and coiled bodies with the nucleolus." @default.
- W4210349767 created "2022-02-08" @default.
- W4210349767 creator A5058438849 @default.
- W4210349767 creator A5086188500 @default.
- W4210349767 date "1966-09-01" @default.
- W4210349767 modified "2023-09-26" @default.
- W4210349767 title "Solubility of ZnSe and ZnTe in Ga and In" @default.
- W4210349767 doi "https://doi.org/10.1016/0038-1098(66)90338-3" @default.
- W4210349767 hasPublicationYear "1966" @default.
- W4210349767 type Work @default.
- W4210349767 citedByCount "0" @default.
- W4210349767 crossrefType "journal-article" @default.
- W4210349767 hasAuthorship W4210349767A5058438849 @default.
- W4210349767 hasAuthorship W4210349767A5086188500 @default.
- W4210349767 hasConcept C104317684 @default.
- W4210349767 hasConcept C112952253 @default.
- W4210349767 hasConcept C11922738 @default.
- W4210349767 hasConcept C142613039 @default.
- W4210349767 hasConcept C153911025 @default.
- W4210349767 hasConcept C183873130 @default.
- W4210349767 hasConcept C190062978 @default.
- W4210349767 hasConcept C2908584439 @default.
- W4210349767 hasConcept C54355233 @default.
- W4210349767 hasConcept C54458228 @default.
- W4210349767 hasConcept C67705224 @default.
- W4210349767 hasConcept C68483431 @default.
- W4210349767 hasConcept C73758832 @default.
- W4210349767 hasConcept C75934600 @default.
- W4210349767 hasConcept C86803240 @default.
- W4210349767 hasConcept C95444343 @default.
- W4210349767 hasConceptScore W4210349767C104317684 @default.
- W4210349767 hasConceptScore W4210349767C112952253 @default.
- W4210349767 hasConceptScore W4210349767C11922738 @default.
- W4210349767 hasConceptScore W4210349767C142613039 @default.
- W4210349767 hasConceptScore W4210349767C153911025 @default.
- W4210349767 hasConceptScore W4210349767C183873130 @default.
- W4210349767 hasConceptScore W4210349767C190062978 @default.
- W4210349767 hasConceptScore W4210349767C2908584439 @default.
- W4210349767 hasConceptScore W4210349767C54355233 @default.
- W4210349767 hasConceptScore W4210349767C54458228 @default.
- W4210349767 hasConceptScore W4210349767C67705224 @default.
- W4210349767 hasConceptScore W4210349767C68483431 @default.
- W4210349767 hasConceptScore W4210349767C73758832 @default.
- W4210349767 hasConceptScore W4210349767C75934600 @default.
- W4210349767 hasConceptScore W4210349767C86803240 @default.
- W4210349767 hasConceptScore W4210349767C95444343 @default.
- W4210349767 hasIssue "9" @default.
- W4210349767 hasLocation W42103497671 @default.
- W4210349767 hasOpenAccess W4210349767 @default.
- W4210349767 hasPrimaryLocation W42103497671 @default.
- W4210349767 hasRelatedWork W1922329782 @default.
- W4210349767 hasRelatedWork W1987403693 @default.
- W4210349767 hasRelatedWork W2037289364 @default.
- W4210349767 hasRelatedWork W2038182020 @default.
- W4210349767 hasRelatedWork W2045351722 @default.
- W4210349767 hasRelatedWork W2067382966 @default.
- W4210349767 hasRelatedWork W2081702026 @default.
- W4210349767 hasRelatedWork W2109552144 @default.
- W4210349767 hasRelatedWork W2110809853 @default.
- W4210349767 hasRelatedWork W2150409766 @default.
- W4210349767 hasVolume "4" @default.
- W4210349767 isParatext "false" @default.
- W4210349767 isRetracted "false" @default.
- W4210349767 workType "article" @default.