Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210362092> ?p ?o ?g. }
- W4210362092 endingPage "e1010294" @default.
- W4210362092 startingPage "e1010294" @default.
- W4210362092 abstract "As the important molecular machinery for membrane protein sorting in eukaryotic cells, the endosomal sorting and transport complexes (ESCRT-0/I/II/III and VPS4) usually participate in various replication stages of enveloped viruses, such as endocytosis and budding. The main subunit of ESCRT-I, Tsg101, has been previously revealed to play a role in the entry and replication of classical swine fever virus (CSFV). However, the effect of the whole ESCRT machinery during CSFV infection has not yet been well defined. Here, we systematically determine the effects of subunits of ESCRT on entry, replication, and budding of CSFV by genetic analysis. We show that EAP20 (VPS25) (ESCRT-II), CHMP4B and CHMP7 (ESCRT-III) regulate CSFV entry and assist vesicles in transporting CSFV from Clathrin, early endosomes, late endosomes to lysosomes. Importantly, we first demonstrate that HRS (ESCRT-0), VPS28 (ESCRT-I), VPS25 (ESCRT-II) and adaptor protein ALIX play important roles in the formation of virus replication complexes (VRC) together with CHMP2B/4B/7 (ESCRT-III), and VPS4A. Further analyses reveal these subunits interact with CSFV nonstructural proteins (NS) and locate in the endoplasmic reticulum, but not Golgi, suggesting the role of ESCRT in regulating VRC assembly. In addition, we demonstrate that VPS4A is close to lipid droplets (LDs), indicating the importance of lipid metabolism in the formation of VRC and nucleic acid production. Altogether, we draw a new picture of cellular ESCRT machinery in CSFV entry and VRC formation, which could provide alternative strategies for preventing and controlling the diseases caused by CSFV or other Pestivirus." @default.
- W4210362092 created "2022-02-08" @default.
- W4210362092 creator A5012625642 @default.
- W4210362092 creator A5018235560 @default.
- W4210362092 creator A5026044398 @default.
- W4210362092 creator A5031465676 @default.
- W4210362092 creator A5032755233 @default.
- W4210362092 creator A5035213402 @default.
- W4210362092 creator A5048379858 @default.
- W4210362092 creator A5062237629 @default.
- W4210362092 creator A5064733360 @default.
- W4210362092 creator A5074960583 @default.
- W4210362092 creator A5078632164 @default.
- W4210362092 creator A5081333862 @default.
- W4210362092 creator A5084457198 @default.
- W4210362092 date "2022-02-04" @default.
- W4210362092 modified "2023-10-16" @default.
- W4210362092 title "Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection" @default.
- W4210362092 cites W1585380514 @default.
- W4210362092 cites W1587896818 @default.
- W4210362092 cites W1754153744 @default.
- W4210362092 cites W1880359614 @default.
- W4210362092 cites W1969067622 @default.
- W4210362092 cites W1976916223 @default.
- W4210362092 cites W1980798766 @default.
- W4210362092 cites W1983686919 @default.
- W4210362092 cites W1990014502 @default.
- W4210362092 cites W1991400645 @default.
- W4210362092 cites W1998079639 @default.
- W4210362092 cites W2025029519 @default.
- W4210362092 cites W2028972269 @default.
- W4210362092 cites W2031935325 @default.
- W4210362092 cites W2042950955 @default.
- W4210362092 cites W2056357144 @default.
- W4210362092 cites W2060078754 @default.
- W4210362092 cites W2070063935 @default.
- W4210362092 cites W2071559289 @default.
- W4210362092 cites W2074130602 @default.
- W4210362092 cites W2078374647 @default.
- W4210362092 cites W2079099220 @default.
- W4210362092 cites W2086827381 @default.
- W4210362092 cites W2091829839 @default.
- W4210362092 cites W2093373040 @default.
- W4210362092 cites W2109900607 @default.
- W4210362092 cites W2115425661 @default.
- W4210362092 cites W2128854173 @default.
- W4210362092 cites W2143210808 @default.
- W4210362092 cites W2152689946 @default.
- W4210362092 cites W2154203178 @default.
- W4210362092 cites W2154717655 @default.
- W4210362092 cites W2163047859 @default.
- W4210362092 cites W2163412375 @default.
- W4210362092 cites W2171626197 @default.
- W4210362092 cites W2179240897 @default.
- W4210362092 cites W2230135021 @default.
- W4210362092 cites W2271346443 @default.
- W4210362092 cites W2288648350 @default.
- W4210362092 cites W2314857906 @default.
- W4210362092 cites W2321038792 @default.
- W4210362092 cites W2476126056 @default.
- W4210362092 cites W2515305272 @default.
- W4210362092 cites W2534760591 @default.
- W4210362092 cites W2784418368 @default.
- W4210362092 cites W2795027349 @default.
- W4210362092 cites W2804568299 @default.
- W4210362092 cites W2809514493 @default.
- W4210362092 cites W2886609030 @default.
- W4210362092 cites W2950913191 @default.
- W4210362092 cites W2951541394 @default.
- W4210362092 cites W2977513631 @default.
- W4210362092 cites W2985101511 @default.
- W4210362092 cites W2985969078 @default.
- W4210362092 cites W3014674608 @default.
- W4210362092 cites W3081264046 @default.
- W4210362092 cites W3093945728 @default.
- W4210362092 cites W3112271045 @default.
- W4210362092 cites W3129827786 @default.
- W4210362092 cites W3132620364 @default.
- W4210362092 cites W3133263773 @default.
- W4210362092 cites W3148478141 @default.
- W4210362092 doi "https://doi.org/10.1371/journal.ppat.1010294" @default.
- W4210362092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35120190" @default.
- W4210362092 hasPublicationYear "2022" @default.
- W4210362092 type Work @default.
- W4210362092 citedByCount "8" @default.
- W4210362092 countsByYear W42103620922023 @default.
- W4210362092 crossrefType "journal-article" @default.
- W4210362092 hasAuthorship W4210362092A5012625642 @default.
- W4210362092 hasAuthorship W4210362092A5018235560 @default.
- W4210362092 hasAuthorship W4210362092A5026044398 @default.
- W4210362092 hasAuthorship W4210362092A5031465676 @default.
- W4210362092 hasAuthorship W4210362092A5032755233 @default.
- W4210362092 hasAuthorship W4210362092A5035213402 @default.
- W4210362092 hasAuthorship W4210362092A5048379858 @default.
- W4210362092 hasAuthorship W4210362092A5062237629 @default.
- W4210362092 hasAuthorship W4210362092A5064733360 @default.
- W4210362092 hasAuthorship W4210362092A5074960583 @default.
- W4210362092 hasAuthorship W4210362092A5078632164 @default.