Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210363045> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W4210363045 endingPage "e057510" @default.
- W4210363045 startingPage "e057510" @default.
- W4210363045 abstract "Cardiolipin (CL) is a mitochondrial phospholipid that may be released extracellularly from damaged or dying cells where it can act as a signaling molecule by interacting with the surrounding cells. Previous research demonstrates that extracellular CL regulates select immune functions of microglia in a toll-like receptor (TLR) 4-dependent manner. However, the effects of CL on astrocyte functions are unknown. Astrocytes are essential participants in diverse physiological brain functions; however, they become overactivated in chronic neuroinflammatory states, including Alzheimer's disease, contributing to damage and death of surrounding tissues and cells. Since astrocytes express TLR 4, we hypothesized that, by interacting with this receptor, extracellular CL modulates the secretion of cytokines and cytotoxins by astrocytes, as well as their phagocytic activity.We studied the effects of CL added to the culture media of primary murine astrocytes and human U118 MG astrocytic cells, which were used as astrocyte models. Fluorescent latex microspheres and synaptosomes were used to investigate the phagocytic activity of cells. The secretion of inflammatory cytokines was measured by the enzyme-linked immunosorbent assay or immunoblotting. U118 MG astrocyte supernatants were transferred to human SH-SY5Y neuronal cells to assess the secretion of cytotoxins. The expression of TLR 4 and glial fibrillary acidic protein (GFAP) by U118 MG astrocytic cells was quantified by immunostaining and immunoblotting, respectively.Extracellular CL alone upregulated the phagocytic activity of human U118 MG astrocytic cells and their secretion of monocyte chemoattractant protein (MCP)-1 and interferon (IFN)-β. Extracellular CL upregulated the phagocytic activity of primary murine astrocytes. TLR 4-specific antagonist TAK-242 inhibited CL-induced secretion of MCP-1 by human U118 MG cells. CL alone upregulated the cell-surface expression of TLR 4 by the same cell type. Extracellular CL inhibited the lipopolysaccharide (LPS)-induced increase in GFAP expression as well as the secretion of cytotoxins by U118 MG astrocytic cells.Extracellular CL may be used to modulate select functions of astrocytes, which become dysregulated in chronic neuroinflammatory states. Therefore, CL, or liposomes containing this phospholipid, could be used to ameliorate the chronic neuroinflammation and adverse astrocyte activation associated with Alzheimer's disease." @default.
- W4210363045 created "2022-02-08" @default.
- W4210363045 creator A5017523393 @default.
- W4210363045 creator A5036891711 @default.
- W4210363045 creator A5046410527 @default.
- W4210363045 creator A5055389633 @default.
- W4210363045 creator A5089558139 @default.
- W4210363045 date "2021-12-01" @default.
- W4210363045 modified "2023-09-29" @default.
- W4210363045 title "Extracellular cardiolipin regulates select immune functions of astrocytes in a toll-like receptor (TLR) 4-dependent manner." @default.
- W4210363045 doi "https://doi.org/10.1002/alz.057510" @default.
- W4210363045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35109221" @default.
- W4210363045 hasPublicationYear "2021" @default.
- W4210363045 type Work @default.
- W4210363045 citedByCount "0" @default.
- W4210363045 crossrefType "journal-article" @default.
- W4210363045 hasAuthorship W4210363045A5017523393 @default.
- W4210363045 hasAuthorship W4210363045A5036891711 @default.
- W4210363045 hasAuthorship W4210363045A5046410527 @default.
- W4210363045 hasAuthorship W4210363045A5055389633 @default.
- W4210363045 hasAuthorship W4210363045A5089558139 @default.
- W4210363045 hasConcept C134018914 @default.
- W4210363045 hasConcept C136449434 @default.
- W4210363045 hasConcept C170493617 @default.
- W4210363045 hasConcept C185592680 @default.
- W4210363045 hasConcept C203014093 @default.
- W4210363045 hasConcept C2776709828 @default.
- W4210363045 hasConcept C2776914184 @default.
- W4210363045 hasConcept C2777542381 @default.
- W4210363045 hasConcept C2779830541 @default.
- W4210363045 hasConcept C28406088 @default.
- W4210363045 hasConcept C49039625 @default.
- W4210363045 hasConcept C529278444 @default.
- W4210363045 hasConcept C55493867 @default.
- W4210363045 hasConcept C86803240 @default.
- W4210363045 hasConcept C87753298 @default.
- W4210363045 hasConcept C8891405 @default.
- W4210363045 hasConcept C95444343 @default.
- W4210363045 hasConceptScore W4210363045C134018914 @default.
- W4210363045 hasConceptScore W4210363045C136449434 @default.
- W4210363045 hasConceptScore W4210363045C170493617 @default.
- W4210363045 hasConceptScore W4210363045C185592680 @default.
- W4210363045 hasConceptScore W4210363045C203014093 @default.
- W4210363045 hasConceptScore W4210363045C2776709828 @default.
- W4210363045 hasConceptScore W4210363045C2776914184 @default.
- W4210363045 hasConceptScore W4210363045C2777542381 @default.
- W4210363045 hasConceptScore W4210363045C2779830541 @default.
- W4210363045 hasConceptScore W4210363045C28406088 @default.
- W4210363045 hasConceptScore W4210363045C49039625 @default.
- W4210363045 hasConceptScore W4210363045C529278444 @default.
- W4210363045 hasConceptScore W4210363045C55493867 @default.
- W4210363045 hasConceptScore W4210363045C86803240 @default.
- W4210363045 hasConceptScore W4210363045C87753298 @default.
- W4210363045 hasConceptScore W4210363045C8891405 @default.
- W4210363045 hasConceptScore W4210363045C95444343 @default.
- W4210363045 hasLocation W42103630451 @default.
- W4210363045 hasOpenAccess W4210363045 @default.
- W4210363045 hasPrimaryLocation W42103630451 @default.
- W4210363045 hasRelatedWork W12534347 @default.
- W4210363045 hasRelatedWork W14780013 @default.
- W4210363045 hasRelatedWork W14838630 @default.
- W4210363045 hasRelatedWork W17474223 @default.
- W4210363045 hasRelatedWork W25776802 @default.
- W4210363045 hasRelatedWork W28750489 @default.
- W4210363045 hasRelatedWork W29710322 @default.
- W4210363045 hasRelatedWork W32416541 @default.
- W4210363045 hasRelatedWork W70766 @default.
- W4210363045 hasRelatedWork W6605826 @default.
- W4210363045 hasVolume "17 Suppl 3" @default.
- W4210363045 isParatext "false" @default.
- W4210363045 isRetracted "false" @default.
- W4210363045 workType "article" @default.