Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210365093> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W4210365093 abstract "As concanavalin A (Con A) has preferential binding specificity for mannose and glucose, here we test the ability of alpha methyl glucose to dissociate yeast (Saccharomyces cerevisiae) from Con A beads. We chose only one concentration of each of these two sugars (mannose sugar in Part I of this series) to study so that we could do hundreds of replicate trials to statistically evaluate the reliability of this new model system. Future dose-response experiments will be performed to precisely determine the effectiveness of the reagents at the lowest concentrations. In 247 repeated trials using 1.5 M alpha methyl glucose versus controls in the absence of sugar, with tiny Standard Errors, the sugar substantially increased dissociation of yeast that were bound to Con A derivatized beads over the 60 min time course compared with sugar-free controls (2-sample-t-test results: 20 min p less than 0.0001; 40 min p less than 0.0001; 60 min p less than 0.0001). Repeated measures ANOVA indicated a significant effect of the treatment on the number of bound yeast (F= 333, p less than 0.0001), as well as a significant interaction between treatment and amount of time elapsed (F=152, p less than 0.0001). The results are expected but the model is new. We believe that for the first time the kinetics of yeast elution from lectin beads is precisely measured in an easily visualized, simple and inexpensive model system to test elution reagents in glycan purification protocols and to test the efficacy of potential carbohydrate-based drugs in dissociating pathogens from human cells (Supported by NIH NIGMS SCORE S0648680, MARC, RISE, the Joseph Drown Foundation and the Sidney Stern Memorial Trust)." @default.
- W4210365093 created "2022-02-08" @default.
- W4210365093 creator A5002857107 @default.
- W4210365093 creator A5003469781 @default.
- W4210365093 creator A5012080988 @default.
- W4210365093 creator A5013461961 @default.
- W4210365093 creator A5013475895 @default.
- W4210365093 creator A5029147477 @default.
- W4210365093 creator A5044539343 @default.
- W4210365093 creator A5044918402 @default.
- W4210365093 creator A5048450263 @default.
- W4210365093 creator A5067265193 @default.
- W4210365093 creator A5072368597 @default.
- W4210365093 creator A5074049142 @default.
- W4210365093 creator A5074353843 @default.
- W4210365093 creator A5080170296 @default.
- W4210365093 creator A5080723957 @default.
- W4210365093 creator A5084230105 @default.
- W4210365093 creator A5090519101 @default.
- W4210365093 date "2010-04-01" @default.
- W4210365093 modified "2023-09-27" @default.
- W4210365093 title "Kinetics of yeast dissociation from lectin beads: II. alpha methyl glucose" @default.
- W4210365093 doi "https://doi.org/10.1096/fasebj.24.1_supplement.480.14" @default.
- W4210365093 hasPublicationYear "2010" @default.
- W4210365093 type Work @default.
- W4210365093 citedByCount "0" @default.
- W4210365093 crossrefType "journal-article" @default.
- W4210365093 hasAuthorship W4210365093A5002857107 @default.
- W4210365093 hasAuthorship W4210365093A5003469781 @default.
- W4210365093 hasAuthorship W4210365093A5012080988 @default.
- W4210365093 hasAuthorship W4210365093A5013461961 @default.
- W4210365093 hasAuthorship W4210365093A5013475895 @default.
- W4210365093 hasAuthorship W4210365093A5029147477 @default.
- W4210365093 hasAuthorship W4210365093A5044539343 @default.
- W4210365093 hasAuthorship W4210365093A5044918402 @default.
- W4210365093 hasAuthorship W4210365093A5048450263 @default.
- W4210365093 hasAuthorship W4210365093A5067265193 @default.
- W4210365093 hasAuthorship W4210365093A5072368597 @default.
- W4210365093 hasAuthorship W4210365093A5074049142 @default.
- W4210365093 hasAuthorship W4210365093A5074353843 @default.
- W4210365093 hasAuthorship W4210365093A5080170296 @default.
- W4210365093 hasAuthorship W4210365093A5080723957 @default.
- W4210365093 hasAuthorship W4210365093A5084230105 @default.
- W4210365093 hasAuthorship W4210365093A5090519101 @default.
- W4210365093 hasConcept C121332964 @default.
- W4210365093 hasConcept C148898269 @default.
- W4210365093 hasConcept C178790620 @default.
- W4210365093 hasConcept C185592680 @default.
- W4210365093 hasConcept C19869320 @default.
- W4210365093 hasConcept C202751555 @default.
- W4210365093 hasConcept C2775887612 @default.
- W4210365093 hasConcept C2777108408 @default.
- W4210365093 hasConcept C2779222958 @default.
- W4210365093 hasConcept C2780758401 @default.
- W4210365093 hasConcept C40875361 @default.
- W4210365093 hasConcept C43617362 @default.
- W4210365093 hasConcept C55493867 @default.
- W4210365093 hasConcept C62520636 @default.
- W4210365093 hasConcept C73826308 @default.
- W4210365093 hasConceptScore W4210365093C121332964 @default.
- W4210365093 hasConceptScore W4210365093C148898269 @default.
- W4210365093 hasConceptScore W4210365093C178790620 @default.
- W4210365093 hasConceptScore W4210365093C185592680 @default.
- W4210365093 hasConceptScore W4210365093C19869320 @default.
- W4210365093 hasConceptScore W4210365093C202751555 @default.
- W4210365093 hasConceptScore W4210365093C2775887612 @default.
- W4210365093 hasConceptScore W4210365093C2777108408 @default.
- W4210365093 hasConceptScore W4210365093C2779222958 @default.
- W4210365093 hasConceptScore W4210365093C2780758401 @default.
- W4210365093 hasConceptScore W4210365093C40875361 @default.
- W4210365093 hasConceptScore W4210365093C43617362 @default.
- W4210365093 hasConceptScore W4210365093C55493867 @default.
- W4210365093 hasConceptScore W4210365093C62520636 @default.
- W4210365093 hasConceptScore W4210365093C73826308 @default.
- W4210365093 hasIssue "S1" @default.
- W4210365093 hasLocation W42103650931 @default.
- W4210365093 hasOpenAccess W4210365093 @default.
- W4210365093 hasPrimaryLocation W42103650931 @default.
- W4210365093 hasRelatedWork W1526970769 @default.
- W4210365093 hasRelatedWork W1794811372 @default.
- W4210365093 hasRelatedWork W1978994833 @default.
- W4210365093 hasRelatedWork W2009215244 @default.
- W4210365093 hasRelatedWork W2084823085 @default.
- W4210365093 hasRelatedWork W2101131499 @default.
- W4210365093 hasRelatedWork W2171307231 @default.
- W4210365093 hasRelatedWork W2185831673 @default.
- W4210365093 hasRelatedWork W2409389032 @default.
- W4210365093 hasRelatedWork W3173904921 @default.
- W4210365093 hasVolume "24" @default.
- W4210365093 isParatext "false" @default.
- W4210365093 isRetracted "false" @default.
- W4210365093 workType "article" @default.