Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210379601> ?p ?o ?g. }
- W4210379601 endingPage "102619" @default.
- W4210379601 startingPage "102619" @default.
- W4210379601 abstract "Cell penetrating peptides (CPPs) have been used as alternative delivery vectors to translocate therapeutic cargo molecules across cell membranes. One example of CPPs is the dTAT peptide, which has shown great promise in the design of highly efficient and low-cytotoxic gene vectors when condensed via “soft” calcium cross links. Here, we investigated the effect of fluorination on the formulation of dTAT complexes and explored their potential for pDNA delivery to cells. Fluorinated dTAT complexes achieve excellent gene transfection efficacy compared to fluorinated PEI polyplexes in A549, HeLa, and MCF-7 cell lines. Furthermore, the fluorinated dTAT complexes exhibit excellent serum resistance, high gene transfection efficacy even in 10% FBS medium, and no detectable cytotoxicity on transfected cells. The optimum NaF concentration (14 mM) resulted in an over 1000-fold enhancement in dTAT complexes (N/P 33) transfection efficiency. According to these findings, fluorination seems to be a potential strategy for creating gene vectors without requiring complex syntheses. • Fluorination has no negative impact on the creation of dTAT complexes. • Incorporation of CaCl 2 and NaF into dTAT complexes exhibit superior improvement in the transfection efficiency. • Fluorinated dTAT complexes show excellent serum resistance andexhibit high transfection efficacy in medium with 10% FBS." @default.
- W4210379601 created "2022-02-08" @default.
- W4210379601 creator A5076154308 @default.
- W4210379601 date "2022-01-01" @default.
- W4210379601 modified "2023-10-16" @default.
- W4210379601 title "The fluorination effect on the transfection efficacy of cell penetrating peptide complexes" @default.
- W4210379601 cites W1515068677 @default.
- W4210379601 cites W1591560031 @default.
- W4210379601 cites W1964146045 @default.
- W4210379601 cites W1968647565 @default.
- W4210379601 cites W1971188178 @default.
- W4210379601 cites W1972128873 @default.
- W4210379601 cites W1975777043 @default.
- W4210379601 cites W1980925538 @default.
- W4210379601 cites W1995770815 @default.
- W4210379601 cites W2001493784 @default.
- W4210379601 cites W2003414091 @default.
- W4210379601 cites W2004349712 @default.
- W4210379601 cites W2007347651 @default.
- W4210379601 cites W2027762479 @default.
- W4210379601 cites W2041244083 @default.
- W4210379601 cites W2045583604 @default.
- W4210379601 cites W2049425569 @default.
- W4210379601 cites W2052633458 @default.
- W4210379601 cites W2055463351 @default.
- W4210379601 cites W2056972670 @default.
- W4210379601 cites W2059751045 @default.
- W4210379601 cites W2065553496 @default.
- W4210379601 cites W2069717129 @default.
- W4210379601 cites W2084283741 @default.
- W4210379601 cites W2087188273 @default.
- W4210379601 cites W2093904928 @default.
- W4210379601 cites W2111490558 @default.
- W4210379601 cites W2114042611 @default.
- W4210379601 cites W2123179916 @default.
- W4210379601 cites W2131471711 @default.
- W4210379601 cites W2151329903 @default.
- W4210379601 cites W2170255011 @default.
- W4210379601 cites W2201498990 @default.
- W4210379601 cites W2281605478 @default.
- W4210379601 cites W2287114143 @default.
- W4210379601 cites W2313088573 @default.
- W4210379601 cites W2323792247 @default.
- W4210379601 cites W2333638833 @default.
- W4210379601 cites W2333945947 @default.
- W4210379601 cites W2338359589 @default.
- W4210379601 cites W2495086507 @default.
- W4210379601 cites W2521389035 @default.
- W4210379601 cites W2547742543 @default.
- W4210379601 cites W2588262749 @default.
- W4210379601 cites W2589725017 @default.
- W4210379601 cites W2590644565 @default.
- W4210379601 cites W2592465512 @default.
- W4210379601 cites W2603828830 @default.
- W4210379601 cites W2614196538 @default.
- W4210379601 cites W2767292607 @default.
- W4210379601 cites W2803996799 @default.
- W4210379601 cites W2804535713 @default.
- W4210379601 cites W2805531856 @default.
- W4210379601 cites W2806666218 @default.
- W4210379601 cites W2895357719 @default.
- W4210379601 cites W2957755849 @default.
- W4210379601 cites W2960810917 @default.
- W4210379601 cites W2988661790 @default.
- W4210379601 cites W3003479630 @default.
- W4210379601 cites W3006422669 @default.
- W4210379601 cites W3007796728 @default.
- W4210379601 cites W3009631698 @default.
- W4210379601 cites W3033346386 @default.
- W4210379601 cites W3036957849 @default.
- W4210379601 cites W3040335627 @default.
- W4210379601 cites W3045647420 @default.
- W4210379601 cites W3108851962 @default.
- W4210379601 cites W3143021599 @default.
- W4210379601 cites W4234018723 @default.
- W4210379601 cites W4249652012 @default.
- W4210379601 cites W2983048398 @default.
- W4210379601 doi "https://doi.org/10.1016/j.plasmid.2022.102619" @default.
- W4210379601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35124112" @default.
- W4210379601 hasPublicationYear "2022" @default.
- W4210379601 type Work @default.
- W4210379601 citedByCount "0" @default.
- W4210379601 crossrefType "journal-article" @default.
- W4210379601 hasAuthorship W4210379601A5076154308 @default.
- W4210379601 hasConcept C104317684 @default.
- W4210379601 hasConcept C109316439 @default.
- W4210379601 hasConcept C12554922 @default.
- W4210379601 hasConcept C135983454 @default.
- W4210379601 hasConcept C1491633281 @default.
- W4210379601 hasConcept C153911025 @default.
- W4210379601 hasConcept C185592680 @default.
- W4210379601 hasConcept C202751555 @default.
- W4210379601 hasConcept C2776929087 @default.
- W4210379601 hasConcept C2777366897 @default.
- W4210379601 hasConcept C2779281246 @default.
- W4210379601 hasConcept C54009773 @default.
- W4210379601 hasConcept C54355233 @default.
- W4210379601 hasConcept C55493867 @default.