FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210442208> ?p ?o ?g. }

• W4210442208 abstract "Abstract Alternative splicing (AS) is necessary for viral proliferation in host cells and a critical regulatory component of viral gene expression. Conventional RNA-seq approaches provide incomplete coverage of AS due to their short read-lengths and are susceptible to biases and artifacts introduced in prevailing library preparation methodologies. Moreover, viral splicing studies are often conducted separately from host cell transcriptome analysis, precluding an assessment of the viral manipulation of host splicing machinery. To address current limitations, we developed a quantitative full-length direct cDNA sequencing strategy to simultaneously profile viral and host cell transcripts. This nanopore-based approach couples processive reverse transcriptases with a novel one-step chemical ablation of 3’ RNA ends (termed CASPR) which decreases ribosomal RNA reads and enriches for poly-adenylated coding sequences. We extensively validate our approach using synthetic reference transcripts and show CASPR doubles the breadth of coverage per transcript and increases detection of long transcripts (>4kb), while being functionally equivalent to PolyA+ selection for transcript quantification. We used our approach to interrogate host cell and HIV-1 transcript dynamics during viral reactivation and identified novel putative HIV-1 host factors containing exon skipping or novel intron retentions and delineated the HIV-1 transcriptional state associated with these differentially regulated host factors." @default.
• W4210442208 created "2022-02-08" @default.
• W4210442208 creator A5031803892 @default.
• W4210442208 creator A5044275707 @default.
• W4210442208 creator A5053820618 @default.
• W4210442208 creator A5063390927 @default.
• W4210442208 date "2022-01-28" @default.
• W4210442208 modified "2023-10-18" @default.
• W4210442208 title "Selective Ablation of 3’ RNA ends and Processive RTs Facilitate Direct cDNA Sequencing of Full-length Host Cell and Viral Transcripts" @default.
• W4210442208 cites W1536601791 @default.
• W4210442208 cites W1866327060 @default.
• W4210442208 cites W1975510543 @default.
• W4210442208 cites W1982692592 @default.
• W4210442208 cites W1984990382 @default.
• W4210442208 cites W1995951039 @default.
• W4210442208 cites W2004652094 @default.
• W4210442208 cites W2010650950 @default.
• W4210442208 cites W2011920499 @default.
• W4210442208 cites W2025001392 @default.
• W4210442208 cites W2029069236 @default.
• W4210442208 cites W2029979788 @default.
• W4210442208 cites W2032136448 @default.
• W4210442208 cites W2032856030 @default.
• W4210442208 cites W2058850627 @default.
• W4210442208 cites W2063530272 @default.
• W4210442208 cites W2063628696 @default.
• W4210442208 cites W2091239909 @default.
• W4210442208 cites W2091581218 @default.
• W4210442208 cites W2091682996 @default.
• W4210442208 cites W2100859269 @default.
• W4210442208 cites W2108234281 @default.
• W4210442208 cites W2122660117 @default.
• W4210442208 cites W2124603155 @default.
• W4210442208 cites W2126492433 @default.
• W4210442208 cites W2133265651 @default.
• W4210442208 cites W2139199979 @default.
• W4210442208 cites W2146942744 @default.
• W4210442208 cites W2155804510 @default.
• W4210442208 cites W2156917581 @default.
• W4210442208 cites W2163580395 @default.
• W4210442208 cites W2276748084 @default.
• W4210442208 cites W2571875121 @default.
• W4210442208 cites W2624877602 @default.
• W4210442208 cites W2625348030 @default.
• W4210442208 cites W2741879909 @default.
• W4210442208 cites W2767788891 @default.
• W4210442208 cites W2781530221 @default.
• W4210442208 cites W2782327081 @default.
• W4210442208 cites W2797781644 @default.
• W4210442208 cites W2802940016 @default.
• W4210442208 cites W2900569176 @default.
• W4210442208 cites W2903983689 @default.
• W4210442208 cites W2923410291 @default.
• W4210442208 cites W2943131312 @default.
• W4210442208 cites W2946370603 @default.
• W4210442208 cites W2950986437 @default.
• W4210442208 cites W2953020124 @default.
• W4210442208 cites W2967902434 @default.
• W4210442208 cites W3000922508 @default.
• W4210442208 cites W3011428862 @default.
• W4210442208 cites W3012867519 @default.
• W4210442208 cites W3015043663 @default.
• W4210442208 cites W3015123524 @default.
• W4210442208 cites W3020765120 @default.
• W4210442208 cites W3022360544 @default.
• W4210442208 cites W3022572641 @default.
• W4210442208 cites W3049393863 @default.
• W4210442208 cites W311927316 @default.
• W4210442208 cites W3152957325 @default.
• W4210442208 cites W4212799224 @default.
• W4210442208 cites W4313314629 @default.
• W4210442208 doi "https://doi.org/10.1101/2022.01.27.478099" @default.
• W4210442208 hasPublicationYear "2022" @default.
• W4210442208 type Work @default.
• W4210442208 citedByCount "0" @default.
• W4210442208 crossrefType "posted-content" @default.
• W4210442208 hasAuthorship W4210442208A5031803892 @default.
• W4210442208 hasAuthorship W4210442208A5044275707 @default.
• W4210442208 hasAuthorship W4210442208A5053820618 @default.
• W4210442208 hasAuthorship W4210442208A5063390927 @default.
• W4210442208 hasBestOaLocation W42104422081 @default.
• W4210442208 hasConcept C104317684 @default.
• W4210442208 hasConcept C150194340 @default.
• W4210442208 hasConcept C162317418 @default.
• W4210442208 hasConcept C187882448 @default.
• W4210442208 hasConcept C194583182 @default.
• W4210442208 hasConcept C36823959 @default.
• W4210442208 hasConcept C54355233 @default.
• W4210442208 hasConcept C54458228 @default.
• W4210442208 hasConcept C67705224 @default.
• W4210442208 hasConcept C70721500 @default.
• W4210442208 hasConcept C86803240 @default.
• W4210442208 hasConcept C94671646 @default.
• W4210442208 hasConceptScore W4210442208C104317684 @default.
• W4210442208 hasConceptScore W4210442208C150194340 @default.
• W4210442208 hasConceptScore W4210442208C162317418 @default.
• W4210442208 hasConceptScore W4210442208C187882448 @default.
• W4210442208 hasConceptScore W4210442208C194583182 @default.
• W4210442208 hasConceptScore W4210442208C36823959 @default.
• W4210442208 hasConceptScore W4210442208C54355233 @default.

• next