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• W4210545031 abstract "Abstract Enterobacter spp. are important nosocomial pathogens responsible of a wide variety of infections, mainly due to Extended Spectrum β-Lactamase (ESBL) producing isolates, constituting a global public health issue in terms of clinical treatment and infection control, especially in low-income countries, where last-line treatment is often unavailable and there is weak nosocomial surveillance. In this study, we conducted a phenotypic and molecular characterization of 8 clinical Enterobacter spp. strains, isolated from patient’s blood in three hospitals in Mozambique. Isolates were identified by MALDI-TOF and antimicrobial Susceptibility Testing was performed by VITEK 2 system. Half of isolates were analyzed by PCR for β-lactamases genes, other isolates by Whole Genome Sequencing. We identified all isolates as Enterobacter cloacae complex (ECC), those from Maputo Central Hospital were polyclonal, multidrug resistant (5/8), and ESBL producers (50%), carrying bla CTX-M-15 and different assortment of bla SHV-12 , bla TEM-1B and bla OXA-1 , and AmpCs bla CMH-3 , bla ACT-7 and bla ACT-9 genes. Resistance determinants linked to fluoroquinolone ( aac(6')Ib-cr and qnrB1 ) and others antimicrobials were also found. Notably, one isolate showed phenotypically resistance to colistin, while another colistin susceptible isolate carried a silent mcr-9 gene. ECC nosocomial surveillance is urgently needed to contain and prevent the dissemination of ESBLs producing clones, and mcr-9 spread to other Enterobacteriaceae." @default.
• W4210545031 created "2022-02-08" @default.
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• W4210545031 date "2022-01-31" @default.
• W4210545031 modified "2023-09-30" @default.
• W4210545031 title "Polyclonal emergence of MDR Enterobacter cloacae complex isolates producing multiple extended spectrum beta-lactamases at Maputo Central Hospital, Mozambique" @default.
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• W4210545031 doi "https://doi.org/10.1007/s12210-021-01039-4" @default.
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