Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210551643> ?p ?o ?g. }
- W4210551643 endingPage "425" @default.
- W4210551643 startingPage "414" @default.
- W4210551643 abstract "Site-specific modification of proteins has important applications in biological research and drug development. Reactive tags such as azide, alkyne, and tetrazine have been used extensively to achieve the abovementioned goal. However, bulky side-chain ligation scars are often left after the labeling and may hinder the biological application of such engineered protein products. Conjugation chemistry via dehydroalanine (Dha) may provide an opportunity for traceless ligation because the activated alkene moiety on Dha can then serve as an electrophile to react with radicalophile, thiol/amine nucleophile, and reactive phosphine probe to introduce a minimal linker in the protein post-translational modifications. In this report, we present a mild and highly efficient enzymatic approach to incorporate Dha with phosphothreonine/serine lyases, OspF and SpvC. These lyases originally catalyze an irreversible elimination reaction that converts a doubly phosphorylated substrate with phosphothreonine (pT) or phosphoserine (pS) to dehydrobutyrine (Dhb) or Dha. To generate a simple monophosphorylated tag for these lyases, we conducted a systematic approach to profile the substrate specificity of OspF and SpvC using peptide arrays and self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry. The optimized tag, [F/Y/W]-pT/pS-[F/Y/W] (where [F/Y/W] indicates an aromatic residue), results in a ∼10-fold enhancement of the overall peptide labeling efficiency via Dha chemistry and enables the first demonstration of protein labeling as well as live cell labeling with a minimal ligation linker via enzyme-mediated incorporation of Dha." @default.
- W4210551643 created "2022-02-08" @default.
- W4210551643 creator A5001359383 @default.
- W4210551643 creator A5015823583 @default.
- W4210551643 creator A5020025866 @default.
- W4210551643 creator A5032120514 @default.
- W4210551643 creator A5033408065 @default.
- W4210551643 creator A5040227293 @default.
- W4210551643 creator A5051853189 @default.
- W4210551643 creator A5053305024 @default.
- W4210551643 creator A5063920141 @default.
- W4210551643 creator A5074169708 @default.
- W4210551643 creator A5075529271 @default.
- W4210551643 date "2022-02-07" @default.
- W4210551643 modified "2023-10-16" @default.
- W4210551643 title "Efficient Enzymatic Incorporation of Dehydroalanine Based on SAMDI-Assisted Identification of Optimized Tags for OspF/SpvC" @default.
- W4210551643 cites W1517600110 @default.
- W4210551643 cites W1607154936 @default.
- W4210551643 cites W1858860241 @default.
- W4210551643 cites W1966497951 @default.
- W4210551643 cites W1973268713 @default.
- W4210551643 cites W1975304761 @default.
- W4210551643 cites W1978513278 @default.
- W4210551643 cites W1994388109 @default.
- W4210551643 cites W2029584311 @default.
- W4210551643 cites W2033098156 @default.
- W4210551643 cites W2053099367 @default.
- W4210551643 cites W2054394841 @default.
- W4210551643 cites W2058907741 @default.
- W4210551643 cites W2067013502 @default.
- W4210551643 cites W2089845283 @default.
- W4210551643 cites W2107003257 @default.
- W4210551643 cites W2107167859 @default.
- W4210551643 cites W2109155760 @default.
- W4210551643 cites W2114520383 @default.
- W4210551643 cites W2127188617 @default.
- W4210551643 cites W2130573571 @default.
- W4210551643 cites W2131215673 @default.
- W4210551643 cites W2131629693 @default.
- W4210551643 cites W2166771133 @default.
- W4210551643 cites W2167023708 @default.
- W4210551643 cites W2254963963 @default.
- W4210551643 cites W2315282308 @default.
- W4210551643 cites W2332024781 @default.
- W4210551643 cites W2335208324 @default.
- W4210551643 cites W2346059527 @default.
- W4210551643 cites W2523580724 @default.
- W4210551643 cites W2527322609 @default.
- W4210551643 cites W2613613983 @default.
- W4210551643 cites W2617704854 @default.
- W4210551643 cites W2667447087 @default.
- W4210551643 cites W2755506814 @default.
- W4210551643 cites W2763278454 @default.
- W4210551643 cites W2763793342 @default.
- W4210551643 cites W2768968558 @default.
- W4210551643 cites W2799876761 @default.
- W4210551643 cites W2802186587 @default.
- W4210551643 cites W2802829327 @default.
- W4210551643 cites W2803269001 @default.
- W4210551643 cites W2807890451 @default.
- W4210551643 cites W2808295078 @default.
- W4210551643 cites W2892934299 @default.
- W4210551643 cites W2916669403 @default.
- W4210551643 cites W2917912064 @default.
- W4210551643 cites W2920212008 @default.
- W4210551643 cites W2923401475 @default.
- W4210551643 cites W2941779667 @default.
- W4210551643 cites W2943183465 @default.
- W4210551643 cites W2975772765 @default.
- W4210551643 cites W2987184321 @default.
- W4210551643 cites W3011480827 @default.
- W4210551643 cites W3087151999 @default.
- W4210551643 cites W3087765001 @default.
- W4210551643 cites W3094631046 @default.
- W4210551643 cites W3103309778 @default.
- W4210551643 cites W3212266162 @default.
- W4210551643 cites W3214597065 @default.
- W4210551643 doi "https://doi.org/10.1021/acschembio.1c00866" @default.
- W4210551643 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35129954" @default.
- W4210551643 hasPublicationYear "2022" @default.
- W4210551643 type Work @default.
- W4210551643 citedByCount "1" @default.
- W4210551643 countsByYear W42105516432023 @default.
- W4210551643 crossrefType "journal-article" @default.
- W4210551643 hasAuthorship W4210551643A5001359383 @default.
- W4210551643 hasAuthorship W4210551643A5015823583 @default.
- W4210551643 hasAuthorship W4210551643A5020025866 @default.
- W4210551643 hasAuthorship W4210551643A5032120514 @default.
- W4210551643 hasAuthorship W4210551643A5033408065 @default.
- W4210551643 hasAuthorship W4210551643A5040227293 @default.
- W4210551643 hasAuthorship W4210551643A5051853189 @default.
- W4210551643 hasAuthorship W4210551643A5053305024 @default.
- W4210551643 hasAuthorship W4210551643A5063920141 @default.
- W4210551643 hasAuthorship W4210551643A5074169708 @default.
- W4210551643 hasAuthorship W4210551643A5075529271 @default.
- W4210551643 hasConcept C111919701 @default.
- W4210551643 hasConcept C161790260 @default.
- W4210551643 hasConcept C185592680 @default.