Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210554875> ?p ?o ?g. }
- W4210554875 endingPage "121391" @default.
- W4210554875 startingPage "121391" @default.
- W4210554875 abstract "Pterygium is an ocular surface disorder with high prevalence that can lead to vision impairment. As a pathological outgrowth of conjunctiva, pterygium involves neovascularization and chronic inflammation. Here, we developed a 3D multicellular in vitro pterygium model using a digital light processing (DLP)-based 3D bioprinting platform with human conjunctival stem cells (hCjSCs). A novel feeder-free culture system was adopted and efficiently expanded the primary hCjSCs with homogeneity, stemness and differentiation potency. The DLP-based 3D bioprinting method was able to fabricate hydrogel scaffolds that support the viability and biological integrity of the encapsulated hCjSCs. The bioprinted 3D pterygium model consisted of hCjSCs, immune cells, and vascular cells to recapitulate the disease microenvironment. Transcriptomic analysis using RNA sequencing (RNA-seq) identified a distinct profile correlated to inflammation response, angiogenesis, and epithelial mesenchymal transition in the bioprinted 3D pterygium model. In addition, the pterygium signatures and disease relevance of the bioprinted model were validated with the public RNA-seq data from patient-derived pterygium tissues. By integrating the stem cell technology with 3D bioprinting, this is the first reported 3D in vitro disease model for pterygium that can be utilized for future studies towards personalized medicine and drug screening." @default.
- W4210554875 created "2022-02-08" @default.
- W4210554875 creator A5006217277 @default.
- W4210554875 creator A5007505462 @default.
- W4210554875 creator A5010071006 @default.
- W4210554875 creator A5014811920 @default.
- W4210554875 creator A5017831020 @default.
- W4210554875 creator A5018690367 @default.
- W4210554875 creator A5030409481 @default.
- W4210554875 creator A5036924943 @default.
- W4210554875 creator A5047905567 @default.
- W4210554875 creator A5053892477 @default.
- W4210554875 creator A5057125192 @default.
- W4210554875 creator A5059316189 @default.
- W4210554875 creator A5066035835 @default.
- W4210554875 creator A5074519587 @default.
- W4210554875 creator A5075883536 @default.
- W4210554875 creator A5081698939 @default.
- W4210554875 creator A5088687625 @default.
- W4210554875 date "2022-03-01" @default.
- W4210554875 modified "2023-10-15" @default.
- W4210554875 title "Rapid 3D bioprinting of a multicellular model recapitulating pterygium microenvironment" @default.
- W4210554875 cites W14065394 @default.
- W4210554875 cites W1506515851 @default.
- W4210554875 cites W1593141554 @default.
- W4210554875 cites W1607966104 @default.
- W4210554875 cites W1760215417 @default.
- W4210554875 cites W1818502761 @default.
- W4210554875 cites W1963501822 @default.
- W4210554875 cites W1964847767 @default.
- W4210554875 cites W1968267566 @default.
- W4210554875 cites W1990069138 @default.
- W4210554875 cites W1998812648 @default.
- W4210554875 cites W2011981225 @default.
- W4210554875 cites W2017161616 @default.
- W4210554875 cites W2034187812 @default.
- W4210554875 cites W2035350115 @default.
- W4210554875 cites W2036146053 @default.
- W4210554875 cites W2037952994 @default.
- W4210554875 cites W2040639812 @default.
- W4210554875 cites W2047872036 @default.
- W4210554875 cites W2054475896 @default.
- W4210554875 cites W2054795756 @default.
- W4210554875 cites W2055956981 @default.
- W4210554875 cites W2061466498 @default.
- W4210554875 cites W2065265148 @default.
- W4210554875 cites W2066538173 @default.
- W4210554875 cites W2074972505 @default.
- W4210554875 cites W2077575831 @default.
- W4210554875 cites W2090276420 @default.
- W4210554875 cites W2102278945 @default.
- W4210554875 cites W2105150792 @default.
- W4210554875 cites W2119034410 @default.
- W4210554875 cites W2122422962 @default.
- W4210554875 cites W2129182574 @default.
- W4210554875 cites W2130410032 @default.
- W4210554875 cites W2139536690 @default.
- W4210554875 cites W2142413049 @default.
- W4210554875 cites W2155186726 @default.
- W4210554875 cites W2159164609 @default.
- W4210554875 cites W2159675211 @default.
- W4210554875 cites W2264718512 @default.
- W4210554875 cites W2339501161 @default.
- W4210554875 cites W2421964473 @default.
- W4210554875 cites W2521465081 @default.
- W4210554875 cites W2529989473 @default.
- W4210554875 cites W2603128961 @default.
- W4210554875 cites W2627051037 @default.
- W4210554875 cites W2751831735 @default.
- W4210554875 cites W2767569544 @default.
- W4210554875 cites W2793613449 @default.
- W4210554875 cites W2805796931 @default.
- W4210554875 cites W2809205410 @default.
- W4210554875 cites W2809246964 @default.
- W4210554875 cites W2890991541 @default.
- W4210554875 cites W2896091135 @default.
- W4210554875 cites W2900044275 @default.
- W4210554875 cites W2902643770 @default.
- W4210554875 cites W2908359749 @default.
- W4210554875 cites W2915166060 @default.
- W4210554875 cites W2921352024 @default.
- W4210554875 cites W2943185211 @default.
- W4210554875 cites W2969669149 @default.
- W4210554875 cites W2981511552 @default.
- W4210554875 cites W2990605218 @default.
- W4210554875 cites W3000456537 @default.
- W4210554875 cites W3004487585 @default.
- W4210554875 cites W3005415926 @default.
- W4210554875 cites W3011511215 @default.
- W4210554875 cites W3013392571 @default.
- W4210554875 cites W3019208434 @default.
- W4210554875 cites W3023456675 @default.
- W4210554875 cites W3033565962 @default.
- W4210554875 cites W3039694096 @default.
- W4210554875 cites W3043815129 @default.
- W4210554875 cites W3087926711 @default.
- W4210554875 cites W3089400224 @default.
- W4210554875 cites W3093929951 @default.