Matches in SemOpenAlex for { <https://semopenalex.org/work/W4210588354> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4210588354 abstract "Abstract Oocyte aneuploidy is caused mainly by chromosome nondisjunction and/or unbalanced sister chromatid pre-division. Although studies in somatic cells have shown that topoisomerase II (TOP2) plays important roles in chromosome condensation and timely separation of centromeres, little is known about its role during oocyte meiosis. Furthermore, because VP-16, which is a TOP2 inhibitor and induces DNA double strand breaks, is often used for ovarian cancer chemotherapy, its effects on oocytes must be studied for ovarian cancer patients to recover ovarian function following chemotherapy. This study showed that inhibiting TOP2 with either ICRF-193 or VP-16 during meiosis I impaired chromatin condensation, chromosome alignment, TOP2α localization and caused metaphase I (MI) arrest and first polar body (PB1) abscission failure. Inhibiting or neutralizing either spindle assembly checkpoint (SAC), Aurora B or maturation-promoting factor (MPF) significantly abolished the effect of ICRF-193 or VP-16 on MI arrest. Treatment with ICRF-193 or VP-16 significantly activated MPF and SAC but the effect disappeared when Aurora B was inhibited. Most of the oocytes matured in the presence of ICRF-193 or VP-16 were arrested at MI, and only 11% to 27% showed PB1 protrusion. Furthermore, most of the PB1 protrusions formed in the presence of ICRF-193 or VP-16 were retracted after further culture for 7 h. In conclusion, TOP2 dysfunction causes MI arrest by activating Aurora B, SAC and MPF and it prevents PB1 abscission by promoting chromatin bridges." @default.
- W4210588354 created "2022-02-08" @default.
- W4210588354 creator A5000223104 @default.
- W4210588354 creator A5007316183 @default.
- W4210588354 creator A5063169546 @default.
- W4210588354 creator A5072208389 @default.
- W4210588354 creator A5073501391 @default.
- W4210588354 creator A5085277740 @default.
- W4210588354 date "2022-01-27" @default.
- W4210588354 modified "2023-10-16" @default.
- W4210588354 title "Topoisomerase II dysfunction causes metaphase I arrest by activating aurora B, SAC and MPF and prevents PB1 abscission in mouse oocytes" @default.
- W4210588354 doi "https://doi.org/10.1093/biolre/ioac011" @default.
- W4210588354 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35084021" @default.
- W4210588354 hasPublicationYear "2022" @default.
- W4210588354 type Work @default.
- W4210588354 citedByCount "0" @default.
- W4210588354 crossrefType "journal-article" @default.
- W4210588354 hasAuthorship W4210588354A5000223104 @default.
- W4210588354 hasAuthorship W4210588354A5007316183 @default.
- W4210588354 hasAuthorship W4210588354A5063169546 @default.
- W4210588354 hasAuthorship W4210588354A5072208389 @default.
- W4210588354 hasAuthorship W4210588354A5073501391 @default.
- W4210588354 hasAuthorship W4210588354A5085277740 @default.
- W4210588354 hasBestOaLocation W42105883541 @default.
- W4210588354 hasConcept C104317684 @default.
- W4210588354 hasConcept C120089663 @default.
- W4210588354 hasConcept C148196450 @default.
- W4210588354 hasConcept C153911025 @default.
- W4210588354 hasConcept C175732170 @default.
- W4210588354 hasConcept C178108356 @default.
- W4210588354 hasConcept C190283241 @default.
- W4210588354 hasConcept C192838845 @default.
- W4210588354 hasConcept C196843134 @default.
- W4210588354 hasConcept C2776690073 @default.
- W4210588354 hasConcept C2779066155 @default.
- W4210588354 hasConcept C2779672484 @default.
- W4210588354 hasConcept C29537977 @default.
- W4210588354 hasConcept C30481170 @default.
- W4210588354 hasConcept C45413362 @default.
- W4210588354 hasConcept C54355233 @default.
- W4210588354 hasConcept C552990157 @default.
- W4210588354 hasConcept C6902291 @default.
- W4210588354 hasConcept C70278784 @default.
- W4210588354 hasConcept C83640560 @default.
- W4210588354 hasConcept C8502773 @default.
- W4210588354 hasConcept C86803240 @default.
- W4210588354 hasConcept C95444343 @default.
- W4210588354 hasConcept C99705001 @default.
- W4210588354 hasConceptScore W4210588354C104317684 @default.
- W4210588354 hasConceptScore W4210588354C120089663 @default.
- W4210588354 hasConceptScore W4210588354C148196450 @default.
- W4210588354 hasConceptScore W4210588354C153911025 @default.
- W4210588354 hasConceptScore W4210588354C175732170 @default.
- W4210588354 hasConceptScore W4210588354C178108356 @default.
- W4210588354 hasConceptScore W4210588354C190283241 @default.
- W4210588354 hasConceptScore W4210588354C192838845 @default.
- W4210588354 hasConceptScore W4210588354C196843134 @default.
- W4210588354 hasConceptScore W4210588354C2776690073 @default.
- W4210588354 hasConceptScore W4210588354C2779066155 @default.
- W4210588354 hasConceptScore W4210588354C2779672484 @default.
- W4210588354 hasConceptScore W4210588354C29537977 @default.
- W4210588354 hasConceptScore W4210588354C30481170 @default.
- W4210588354 hasConceptScore W4210588354C45413362 @default.
- W4210588354 hasConceptScore W4210588354C54355233 @default.
- W4210588354 hasConceptScore W4210588354C552990157 @default.
- W4210588354 hasConceptScore W4210588354C6902291 @default.
- W4210588354 hasConceptScore W4210588354C70278784 @default.
- W4210588354 hasConceptScore W4210588354C83640560 @default.
- W4210588354 hasConceptScore W4210588354C8502773 @default.
- W4210588354 hasConceptScore W4210588354C86803240 @default.
- W4210588354 hasConceptScore W4210588354C95444343 @default.
- W4210588354 hasConceptScore W4210588354C99705001 @default.
- W4210588354 hasLocation W42105883541 @default.
- W4210588354 hasLocation W42105883542 @default.
- W4210588354 hasOpenAccess W4210588354 @default.
- W4210588354 hasPrimaryLocation W42105883541 @default.
- W4210588354 hasRelatedWork W1113861 @default.
- W4210588354 hasRelatedWork W20295496 @default.
- W4210588354 hasRelatedWork W20316631 @default.
- W4210588354 hasRelatedWork W2732359 @default.
- W4210588354 hasRelatedWork W28997910 @default.
- W4210588354 hasRelatedWork W33256975 @default.
- W4210588354 hasRelatedWork W3496144 @default.
- W4210588354 hasRelatedWork W42375930 @default.
- W4210588354 hasRelatedWork W44676527 @default.
- W4210588354 hasRelatedWork W9425716 @default.
- W4210588354 isParatext "false" @default.
- W4210588354 isRetracted "false" @default.
- W4210588354 workType "article" @default.