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- W4210590790 abstract "Long-term measurements may be important to monitor waning SARS-CoV-2 immune responses after infection or vaccination. Viral neutralization assays provide a functional measure of SARS-CoV-2 immunity but are complex to perform on large populations. We sought to determine whether a dichotomous lateral flow or semi-quantitative immunoassay can predict viral neutralization activity We enrolled 98 individuals with a history of SARS-CoV-2 infection and followed them for approximately 6 months via 2 research visits. After informed consent, serum was obtained at each visit. The Centaur® SARS-CoV-2 Total Assay was used to detect spike antibodies. The Healgen Scientific® lateral flow assay was used to detect IgG and IgM spike antibody responses. HEK-293T cells infected with SARS-CoV-2-pseudotyped lentiviral particles were used to perform viral pseudo-neutralization assays. When using a viral neutralization threshold activity of at least of 10% of the positive control, the spike immunoassay provided 91% sensitivity and 27% specificity when examined a mean of 52 days post-infection; the lateral flow assay spike IgG provided 99% sensitivity and 36% specificity. At a later timepoint, a mean of 192 days post-infection, the spike immunoassay provided 99% sensitivity and 24% specificity; the lateral flow assay spike IgG provided 97% sensitivity and 48% specificity. The spike immunoassay and lateral flow assay provided excellent sensitivity for detecting viral neutralization activity. Specificity was limited due to few negative controls evaluated and should improve with additional samples. This suggests that simple, low complexity antibody tests could act as a first screening test for SARS-CoV-2 viral neutralization activity over time." @default.
- W4210590790 created "2022-02-08" @default.
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- W4210590790 date "2022-02-01" @default.
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- W4210590790 title "Spike Antibody Assays Can Screen For Viral Neutralizing Activity Toward SARS-CoV-2" @default.
- W4210590790 doi "https://doi.org/10.1016/j.jaci.2021.12.352" @default.
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