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- W4211025265 abstract "Voltage-dependent potassium channel Kv1.3 plays a key role on T cell activation; however, lack of reliable antibodies has prevented its accurate detection under endogenous circumstances. To overcome this limitation, we created a T cell-derived Jurkat cell line with endogenous Kv1.3 channel tagged in order to determine expression, location and changes upon activation of the native Kv1.3 channels. CRISPR-Cas9 technique was used to generate a double strand break on the gene locus of KCNA3 and a DNA-donor to insert a Flag-Myc peptide at the C-terminus directed by homologous recombination." @default.
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- W4211025265 date "2022-02-01" @default.
- W4211025265 modified "2023-10-18" @default.
- W4211025265 title "Characterization of endogenous Kv1.3 channel isoforms in T cells" @default.
- W4211025265 doi "https://doi.org/10.1016/j.bpj.2021.11.821" @default.
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