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- W4211034319 abstract "Cryo-electron tomography (cryo-ET) enables reconstructions of 3-dimensional views of the membranes, filaments, and macromolecular complexes in a cell with subnanometer resolution, and the advent of cryo-focused ion beam milling has enabled imaging in sections of cells that would otherwise be too thick for electron transmission. We present a semi-automated workflow to segment membranes and reconstruct their underlying surface geometry in cryo-ET. This workflow allows the straightforward reconstruction of even very complex and high curvature membranes, such as the endoplasmic reticulum and the mitochondrial inner membrane cristae, with high fidelity." @default.
- W4211034319 created "2022-02-13" @default.
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- W4211034319 date "2022-02-01" @default.
- W4211034319 modified "2023-09-27" @default.
- W4211034319 title "Modeling the complex geometry of organelle membranes inside cells with cryo-electron tomography" @default.
- W4211034319 doi "https://doi.org/10.1016/j.bpj.2021.11.304" @default.
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