Matches in SemOpenAlex for { <https://semopenalex.org/work/W4211040588> ?p ?o ?g. }
- W4211040588 abstract "Allostery enables proteins to interconvert different biochemical signals and form complex metabolic and signaling networks. We hypothesize that circular permutation of proteins increases the probability of functional coupling of new N- and C- termini with the protein's active center through increased local structural disorder. To test this we construct a synthetically allosteric version of circular permutated NanoLuc luciferase that can be activated through ligand-induced intramolecular non-covalent cyclisation. This switch module is tolerant of the structure of binding domains and their ligands, and can be used to create biosensors of proteins and small molecules. The developed biosensors covers a range of emission wavelengths and displays sensitivity as low as 50pM and dynamic range as high as 16-fold and could quantify their cognate ligand in human fluids. We apply hydrogen exchange kinetic mass spectroscopy to analyze time resolved structural changes in the developed biosensors and observe ligand-mediated folding of newly created termini." @default.
- W4211040588 created "2022-02-13" @default.
- W4211040588 creator A5002859667 @default.
- W4211040588 creator A5004297533 @default.
- W4211040588 creator A5011553529 @default.
- W4211040588 creator A5018214782 @default.
- W4211040588 creator A5029856303 @default.
- W4211040588 creator A5048093771 @default.
- W4211040588 creator A5057850740 @default.
- W4211040588 creator A5063293727 @default.
- W4211040588 creator A5074825590 @default.
- W4211040588 creator A5088252643 @default.
- W4211040588 creator A5090719381 @default.
- W4211040588 date "2022-02-10" @default.
- W4211040588 modified "2023-09-26" @default.
- W4211040588 title "Engineering and exploiting synthetic allostery of NanoLuc luciferase" @default.
- W4211040588 cites W1964439982 @default.
- W4211040588 cites W1968830268 @default.
- W4211040588 cites W1970877840 @default.
- W4211040588 cites W2014252415 @default.
- W4211040588 cites W2025767029 @default.
- W4211040588 cites W2028910937 @default.
- W4211040588 cites W2031264524 @default.
- W4211040588 cites W2044476679 @default.
- W4211040588 cites W2048072440 @default.
- W4211040588 cites W2058922193 @default.
- W4211040588 cites W2061089306 @default.
- W4211040588 cites W2065884669 @default.
- W4211040588 cites W2066322883 @default.
- W4211040588 cites W2068384462 @default.
- W4211040588 cites W2070395999 @default.
- W4211040588 cites W2070582998 @default.
- W4211040588 cites W2075028016 @default.
- W4211040588 cites W2080169144 @default.
- W4211040588 cites W2087276894 @default.
- W4211040588 cites W2088345550 @default.
- W4211040588 cites W2094777850 @default.
- W4211040588 cites W2100295737 @default.
- W4211040588 cites W2114320763 @default.
- W4211040588 cites W2115916264 @default.
- W4211040588 cites W2133872268 @default.
- W4211040588 cites W2138325877 @default.
- W4211040588 cites W2147196684 @default.
- W4211040588 cites W2160536323 @default.
- W4211040588 cites W2176085780 @default.
- W4211040588 cites W2226712470 @default.
- W4211040588 cites W2291572297 @default.
- W4211040588 cites W2298846860 @default.
- W4211040588 cites W2313892377 @default.
- W4211040588 cites W2321115852 @default.
- W4211040588 cites W2358670987 @default.
- W4211040588 cites W2554586955 @default.
- W4211040588 cites W2567498952 @default.
- W4211040588 cites W2573203309 @default.
- W4211040588 cites W2588375557 @default.
- W4211040588 cites W2616441438 @default.
- W4211040588 cites W2624558925 @default.
- W4211040588 cites W2737510383 @default.
- W4211040588 cites W2745087918 @default.
- W4211040588 cites W2762394669 @default.
- W4211040588 cites W2783429882 @default.
- W4211040588 cites W2791574358 @default.
- W4211040588 cites W2888916742 @default.
- W4211040588 cites W2890084910 @default.
- W4211040588 cites W2893090773 @default.
- W4211040588 cites W2905098961 @default.
- W4211040588 cites W2907580401 @default.
- W4211040588 cites W2911398658 @default.
- W4211040588 cites W2911724959 @default.
- W4211040588 cites W2914975766 @default.
- W4211040588 cites W2937203694 @default.
- W4211040588 cites W2944046761 @default.
- W4211040588 cites W2944809780 @default.
- W4211040588 cites W2955583983 @default.
- W4211040588 cites W2969871064 @default.
- W4211040588 cites W2971164558 @default.
- W4211040588 cites W3020319586 @default.
- W4211040588 cites W3113726563 @default.
- W4211040588 cites W3125484908 @default.
- W4211040588 cites W3127497789 @default.
- W4211040588 cites W3128787757 @default.
- W4211040588 cites W3137813344 @default.
- W4211040588 cites W3171070535 @default.
- W4211040588 cites W3186022502 @default.
- W4211040588 cites W3199721927 @default.
- W4211040588 cites W3205059806 @default.
- W4211040588 cites W2947053453 @default.
- W4211040588 doi "https://doi.org/10.1038/s41467-022-28425-2" @default.
- W4211040588 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35145068" @default.
- W4211040588 hasPublicationYear "2022" @default.
- W4211040588 type Work @default.
- W4211040588 citedByCount "18" @default.
- W4211040588 countsByYear W42110405882022 @default.
- W4211040588 countsByYear W42110405882023 @default.
- W4211040588 crossrefType "journal-article" @default.
- W4211040588 hasAuthorship W4211040588A5002859667 @default.
- W4211040588 hasAuthorship W4211040588A5004297533 @default.
- W4211040588 hasAuthorship W4211040588A5011553529 @default.
- W4211040588 hasAuthorship W4211040588A5018214782 @default.
- W4211040588 hasAuthorship W4211040588A5029856303 @default.