Matches in SemOpenAlex for { <https://semopenalex.org/work/W4211083037> ?p ?o ?g. }
- W4211083037 endingPage "1974.e6" @default.
- W4211083037 startingPage "1962" @default.
- W4211083037 abstract "Background & AimsIncreased colonic serotonin (5-HT) level and decreased serotonin reuptake transporter (SERT) expression in irritable bowel syndrome (IBS) may contribute to diarrhea and visceral hypersensitivity. We investigated whether mucosal SERT is modulated by gut microbiota via a mast cell–prostaglandin E2 (PGE2) pathway.MethodsC57Bl/6 mice received intracolonic infusion of fecal supernatant (FS) from healthy controls or patients with diarrhea-predominant irritable bowel syndrome (IBS-D). The role of mast cells was studied in mast cell–deficient mice. Colonic organoids and/or mast cells were used for in vitro experiments. SERT expression was measured by quantitative polymerase chain reaction and Western blot. Visceromotor responses to colorectal distension and colonic transit were assessed.ResultsIntracolonic infusion of IBS-D FS in mice caused an increase in mucosal 5-HT compared with healthy control FS, accompanied by ∼50% reduction in SERT expression. Mast cell stabilizers, cyclooxygenase-2 inhibitors, and PGE2 receptor antagonist prevented SERT downregulation. Intracolonic infusion of IBS-D FS failed to reduce SERT expression in mast cell–deficient (W/Wv) mice. This response was restored by mast cell reconstitution. The downregulation of SERT expression evoked by IBS FS was prevented by lipopolysaccharide (LPS) antagonist LPS from Rhodobacter sphaeroides and a bacterial trypsin inhibitor. In vitro LPS treatment caused increased cyclooxygenase-2 expression and PGE2 release from cultured mouse mast cells. Intracolonic infusion of IBS-D FS in mice reduced colonic transit, increased fecal water content, and increased visceromotor responses to colorectal distension. Ondansetron prevented these changes.ConclusionsFecal LPS acting in concert with trypsin in patients with IBS-D stimulates mucosal mast cells to release PGE2, which downregulates mucosal SERT, resulting in increased mucosal 5-HT. This may contribute to diarrhea and abdominal pain common in IBS. Increased colonic serotonin (5-HT) level and decreased serotonin reuptake transporter (SERT) expression in irritable bowel syndrome (IBS) may contribute to diarrhea and visceral hypersensitivity. We investigated whether mucosal SERT is modulated by gut microbiota via a mast cell–prostaglandin E2 (PGE2) pathway. C57Bl/6 mice received intracolonic infusion of fecal supernatant (FS) from healthy controls or patients with diarrhea-predominant irritable bowel syndrome (IBS-D). The role of mast cells was studied in mast cell–deficient mice. Colonic organoids and/or mast cells were used for in vitro experiments. SERT expression was measured by quantitative polymerase chain reaction and Western blot. Visceromotor responses to colorectal distension and colonic transit were assessed. Intracolonic infusion of IBS-D FS in mice caused an increase in mucosal 5-HT compared with healthy control FS, accompanied by ∼50% reduction in SERT expression. Mast cell stabilizers, cyclooxygenase-2 inhibitors, and PGE2 receptor antagonist prevented SERT downregulation. Intracolonic infusion of IBS-D FS failed to reduce SERT expression in mast cell–deficient (W/Wv) mice. This response was restored by mast cell reconstitution. The downregulation of SERT expression evoked by IBS FS was prevented by lipopolysaccharide (LPS) antagonist LPS from Rhodobacter sphaeroides and a bacterial trypsin inhibitor. In vitro LPS treatment caused increased cyclooxygenase-2 expression and PGE2 release from cultured mouse mast cells. Intracolonic infusion of IBS-D FS in mice reduced colonic transit, increased fecal water content, and increased visceromotor responses to colorectal distension. Ondansetron prevented these changes. Fecal LPS acting in concert with trypsin in patients with IBS-D stimulates mucosal mast cells to release PGE2, which downregulates mucosal SERT, resulting in increased mucosal 5-HT. This may contribute to diarrhea and abdominal pain common in IBS." @default.
- W4211083037 created "2022-02-13" @default.
- W4211083037 creator A5020139593 @default.
- W4211083037 creator A5042087307 @default.
- W4211083037 creator A5057216753 @default.
- W4211083037 creator A5086272334 @default.
- W4211083037 date "2022-06-01" @default.
- W4211083037 modified "2023-10-17" @default.
- W4211083037 title "Mucosal Serotonin Reuptake Transporter Expression in Irritable Bowel Syndrome Is Modulated by Gut Microbiota Via Mast Cell–Prostaglandin E2" @default.
- W4211083037 cites W1519585418 @default.
- W4211083037 cites W1738485982 @default.
- W4211083037 cites W1897161389 @default.
- W4211083037 cites W1907864108 @default.
- W4211083037 cites W1963579052 @default.
- W4211083037 cites W1965551406 @default.
- W4211083037 cites W1969144895 @default.
- W4211083037 cites W1970035176 @default.
- W4211083037 cites W1976859322 @default.
- W4211083037 cites W1992000894 @default.
- W4211083037 cites W1997242740 @default.
- W4211083037 cites W2016520429 @default.
- W4211083037 cites W2018000139 @default.
- W4211083037 cites W2024859421 @default.
- W4211083037 cites W2033386838 @default.
- W4211083037 cites W2043323520 @default.
- W4211083037 cites W2060033529 @default.
- W4211083037 cites W2073519139 @default.
- W4211083037 cites W2079527929 @default.
- W4211083037 cites W2085139899 @default.
- W4211083037 cites W2090377492 @default.
- W4211083037 cites W2097785193 @default.
- W4211083037 cites W2099763225 @default.
- W4211083037 cites W2105022360 @default.
- W4211083037 cites W2116314888 @default.
- W4211083037 cites W2116823408 @default.
- W4211083037 cites W2121087428 @default.
- W4211083037 cites W2126853519 @default.
- W4211083037 cites W2128077096 @default.
- W4211083037 cites W2129921159 @default.
- W4211083037 cites W2132910924 @default.
- W4211083037 cites W2136043481 @default.
- W4211083037 cites W2145135755 @default.
- W4211083037 cites W2149934511 @default.
- W4211083037 cites W2156345396 @default.
- W4211083037 cites W2158148601 @default.
- W4211083037 cites W2160204304 @default.
- W4211083037 cites W2160864720 @default.
- W4211083037 cites W2167117654 @default.
- W4211083037 cites W2167343594 @default.
- W4211083037 cites W2203163745 @default.
- W4211083037 cites W2286771948 @default.
- W4211083037 cites W2318140663 @default.
- W4211083037 cites W2460164747 @default.
- W4211083037 cites W2561402640 @default.
- W4211083037 cites W2612522158 @default.
- W4211083037 cites W2658508676 @default.
- W4211083037 cites W2769660358 @default.
- W4211083037 cites W2783139292 @default.
- W4211083037 cites W2809503140 @default.
- W4211083037 cites W2912100273 @default.
- W4211083037 cites W3008887081 @default.
- W4211083037 cites W3044383360 @default.
- W4211083037 doi "https://doi.org/10.1053/j.gastro.2022.02.016" @default.
- W4211083037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35167867" @default.
- W4211083037 hasPublicationYear "2022" @default.
- W4211083037 type Work @default.
- W4211083037 citedByCount "21" @default.
- W4211083037 countsByYear W42110830372022 @default.
- W4211083037 countsByYear W42110830372023 @default.
- W4211083037 crossrefType "journal-article" @default.
- W4211083037 hasAuthorship W4211083037A5020139593 @default.
- W4211083037 hasAuthorship W4211083037A5042087307 @default.
- W4211083037 hasAuthorship W4211083037A5057216753 @default.
- W4211083037 hasAuthorship W4211083037A5086272334 @default.
- W4211083037 hasBestOaLocation W42110830371 @default.
- W4211083037 hasConcept C104317684 @default.
- W4211083037 hasConcept C126322002 @default.
- W4211083037 hasConcept C127561419 @default.
- W4211083037 hasConcept C134018914 @default.
- W4211083037 hasConcept C170493617 @default.
- W4211083037 hasConcept C185592680 @default.
- W4211083037 hasConcept C203014093 @default.
- W4211083037 hasConcept C2775864247 @default.
- W4211083037 hasConcept C2777956040 @default.
- W4211083037 hasConcept C2778271842 @default.
- W4211083037 hasConcept C2779726688 @default.
- W4211083037 hasConcept C55493867 @default.
- W4211083037 hasConcept C71924100 @default.
- W4211083037 hasConcept C98274493 @default.
- W4211083037 hasConceptScore W4211083037C104317684 @default.
- W4211083037 hasConceptScore W4211083037C126322002 @default.
- W4211083037 hasConceptScore W4211083037C127561419 @default.
- W4211083037 hasConceptScore W4211083037C134018914 @default.
- W4211083037 hasConceptScore W4211083037C170493617 @default.
- W4211083037 hasConceptScore W4211083037C185592680 @default.
- W4211083037 hasConceptScore W4211083037C203014093 @default.
- W4211083037 hasConceptScore W4211083037C2775864247 @default.
- W4211083037 hasConceptScore W4211083037C2777956040 @default.