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- W4211132012 abstract "This paper presents a microfluidic platform to measure three types of proteins of single cells where individual cells stained with fluorescence labelled antibodies were co-encapsulated with proteinase K, which stripped bound antibodies to generate even distributions of fluorescence within droplets. Based on the equivalent volume of the detection region formed by constriction microchannels, fluorescent measurements of droplets encapsulating single cells and pure antibody solutions were correlated with each other. Based on this approach, expressions of β-actin, α-tubulin and β-tubulin of CAL 27 cells were quantified as 1.15±0.53×10 <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>6</sup> , 2.43±1.39×10 <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>5</sup> and 2.29±0.98×10 <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>5</sup> ( <tex xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>$N$</tex> <inf xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>cell</inf> =2 869), providing a new perspective for single-cell analysis." @default.
- W4211132012 created "2022-02-13" @default.
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- W4211132012 date "2022-01-09" @default.
- W4211132012 modified "2023-09-27" @default.
- W4211132012 title "Development of Droplet Microfluidics Enabling Quantitative Measurements of Multiple Proteions at Single-Cell Level" @default.
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- W4211132012 doi "https://doi.org/10.1109/mems51670.2022.9699470" @default.
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