Matches in SemOpenAlex for { <https://semopenalex.org/work/W4211202786> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W4211202786 abstract "Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry yields quantitative RNA secondary and tertiary structure information at single nucleotide resolution. SHAPE takes advantage of the discovery that the nucleophilic reactivity of the ribose 2'-hydroxyl group is modulated by local nucleotide flexibility in the RNA backbone. Flexible nucleotides are reactive toward hydroxyl-selective electrophiles, whereas constrained nucleotides are unreactive. Initial versions of SHAPE chemistry, which employ isatoic anhydride derivatives that react on the minute time scale, are emerging as the ideal technology for monitoring equilibrium structures of RNA in a wide variety of biological environments. Here, we extend SHAPE chemistry to a benzoyl cyanide scaffold to make possible facile time-resolved kinetic studies of RNA in approximately 1 s snapshots. We then use SHAPE chemistry to follow the time-dependent folding of an RNase P specificity domain RNA. Tertiary interactions form in two distinct steps with local tertiary contacts forming an order of magnitude faster than long-range interactions. Rate-determining tertiary folding requires minutes despite that no non-native interactions must be disrupted to form the native structure. Instead, overall folding is limited by simultaneous formation of interactions approximately 55 A distant in the RNA. Time-resolved SHAPE holds broad potential for understanding structural biogenesis and the conformational interconversions essential to the functions of complex RNA molecules at single nucleotide resolution. PMID: 18998638" @default.
- W4211202786 created "2022-02-13" @default.
- W4211202786 creator A5086295306 @default.
- W4211202786 date "2008-12-02" @default.
- W4211202786 modified "2023-09-26" @default.
- W4211202786 title "Faculty Opinions recommendation of Time-resolved RNA SHAPE chemistry." @default.
- W4211202786 doi "https://doi.org/10.3410/f.1131907.589011" @default.
- W4211202786 hasPublicationYear "2008" @default.
- W4211202786 type Work @default.
- W4211202786 citedByCount "0" @default.
- W4211202786 crossrefType "dataset" @default.
- W4211202786 hasAuthorship W4211202786A5086295306 @default.
- W4211202786 hasConcept C104317684 @default.
- W4211202786 hasConcept C119599485 @default.
- W4211202786 hasConcept C126142528 @default.
- W4211202786 hasConcept C127413603 @default.
- W4211202786 hasConcept C148625055 @default.
- W4211202786 hasConcept C161790260 @default.
- W4211202786 hasConcept C178907741 @default.
- W4211202786 hasConcept C181199279 @default.
- W4211202786 hasConcept C185592680 @default.
- W4211202786 hasConcept C193557335 @default.
- W4211202786 hasConcept C194993378 @default.
- W4211202786 hasConcept C21951064 @default.
- W4211202786 hasConcept C2776545253 @default.
- W4211202786 hasConcept C2779461342 @default.
- W4211202786 hasConcept C512185932 @default.
- W4211202786 hasConcept C552990157 @default.
- W4211202786 hasConcept C55493867 @default.
- W4211202786 hasConcept C61234307 @default.
- W4211202786 hasConcept C67705224 @default.
- W4211202786 hasConcept C71240020 @default.
- W4211202786 hasConcept C76346623 @default.
- W4211202786 hasConceptScore W4211202786C104317684 @default.
- W4211202786 hasConceptScore W4211202786C119599485 @default.
- W4211202786 hasConceptScore W4211202786C126142528 @default.
- W4211202786 hasConceptScore W4211202786C127413603 @default.
- W4211202786 hasConceptScore W4211202786C148625055 @default.
- W4211202786 hasConceptScore W4211202786C161790260 @default.
- W4211202786 hasConceptScore W4211202786C178907741 @default.
- W4211202786 hasConceptScore W4211202786C181199279 @default.
- W4211202786 hasConceptScore W4211202786C185592680 @default.
- W4211202786 hasConceptScore W4211202786C193557335 @default.
- W4211202786 hasConceptScore W4211202786C194993378 @default.
- W4211202786 hasConceptScore W4211202786C21951064 @default.
- W4211202786 hasConceptScore W4211202786C2776545253 @default.
- W4211202786 hasConceptScore W4211202786C2779461342 @default.
- W4211202786 hasConceptScore W4211202786C512185932 @default.
- W4211202786 hasConceptScore W4211202786C552990157 @default.
- W4211202786 hasConceptScore W4211202786C55493867 @default.
- W4211202786 hasConceptScore W4211202786C61234307 @default.
- W4211202786 hasConceptScore W4211202786C67705224 @default.
- W4211202786 hasConceptScore W4211202786C71240020 @default.
- W4211202786 hasConceptScore W4211202786C76346623 @default.
- W4211202786 hasLocation W42112027861 @default.
- W4211202786 hasOpenAccess W4211202786 @default.
- W4211202786 hasPrimaryLocation W42112027861 @default.
- W4211202786 hasRelatedWork W2092422591 @default.
- W4211202786 hasRelatedWork W2118501435 @default.
- W4211202786 hasRelatedWork W2132589661 @default.
- W4211202786 hasRelatedWork W2134036931 @default.
- W4211202786 hasRelatedWork W2486983295 @default.
- W4211202786 hasRelatedWork W2900607420 @default.
- W4211202786 hasRelatedWork W2909463616 @default.
- W4211202786 hasRelatedWork W2912431544 @default.
- W4211202786 hasRelatedWork W2954718734 @default.
- W4211202786 hasRelatedWork W3046411315 @default.
- W4211202786 isParatext "false" @default.
- W4211202786 isRetracted "false" @default.
- W4211202786 workType "dataset" @default.