Matches in SemOpenAlex for { <https://semopenalex.org/work/W4212778719> ?p ?o ?g. }
- W4212778719 endingPage "6124" @default.
- W4212778719 startingPage "6115" @default.
- W4212778719 abstract "Catalytic hydrolysis of cyclic guanosine monophosphate (cGMP) by phosphodiesterase 6 (PDE6) is critical in phototransduction signalling in photoreceptors. Mutations in the genes encoding any of the three PDE6 subunits are associated with retinitis pigmentosa, the most common form of inherited retinal diseases. The RD1 mouse carries a naturally occurring nonsense mutation in the Pde6b gene. The RD1 mouse retina rapidly degenerates and fails to form rod photoreceptor outer segments due to the elevated cGMP level and subsequent excessive Ca2+ influx. In this study, we aim to test whether the PDE5 expression, a non-photoreceptor-specific member of the PDE superfamily, rescues photoreceptors in the RD1 retina.Electroporation used the PDE5 expression plasmid to transfect neonatal RD1 mice. The mouse retina degeneration was assessed by retinal sections' stains with DAPI. The expression and localization of phototransduction proteins in photoreceptors were analysed by immunostaining. The expression of proteins in cultured cells was analysed by immunoblotting.The exogenous PDE5 expression, a non-photoreceptor-specific member of the PDE superfamily, prevents photoreceptor degeneration in RD1 mice. Unlike endogenous photoreceptor-specific PDE6 localised in the outer segments of photoreceptors, ectopically- expressed PDE5 was distributed in inner segments and synaptic terminals. PDE5 also promoted the development of the outer segments in RD1 mice. PDE5 co-expression with rhodopsin in cultured cells showed enhanced rhodopsin expression.Lowering the cGMP level in photoreceptors by PDE5 is sufficient to rescue photoreceptors in RD1 retinas. cGMP may also play a role in rhodopsin expression regulation in photoreceptors." @default.
- W4212778719 created "2022-02-24" @default.
- W4212778719 creator A5007286842 @default.
- W4212778719 creator A5016402345 @default.
- W4212778719 creator A5055426982 @default.
- W4212778719 creator A5060902570 @default.
- W4212778719 creator A5063850713 @default.
- W4212778719 creator A5085329149 @default.
- W4212778719 date "2022-12-01" @default.
- W4212778719 modified "2023-10-14" @default.
- W4212778719 title "Exogenous PDE5 Expression Rescues Photoreceptors in RD1 Mice" @default.
- W4212778719 cites W111988334 @default.
- W4212778719 cites W1514240524 @default.
- W4212778719 cites W1556624435 @default.
- W4212778719 cites W1759773091 @default.
- W4212778719 cites W1836887890 @default.
- W4212778719 cites W1869250499 @default.
- W4212778719 cites W1968594982 @default.
- W4212778719 cites W1968686595 @default.
- W4212778719 cites W1968800607 @default.
- W4212778719 cites W1978070679 @default.
- W4212778719 cites W1978809540 @default.
- W4212778719 cites W1987826983 @default.
- W4212778719 cites W1990844378 @default.
- W4212778719 cites W1994963086 @default.
- W4212778719 cites W1995580942 @default.
- W4212778719 cites W1997065647 @default.
- W4212778719 cites W2006226511 @default.
- W4212778719 cites W2014944159 @default.
- W4212778719 cites W2019422832 @default.
- W4212778719 cites W2020239941 @default.
- W4212778719 cites W2025364863 @default.
- W4212778719 cites W2025789164 @default.
- W4212778719 cites W2027588113 @default.
- W4212778719 cites W2031604547 @default.
- W4212778719 cites W2037709331 @default.
- W4212778719 cites W2041102000 @default.
- W4212778719 cites W2043408047 @default.
- W4212778719 cites W2062742450 @default.
- W4212778719 cites W2064268803 @default.
- W4212778719 cites W2064304960 @default.
- W4212778719 cites W2077274995 @default.
- W4212778719 cites W2080382431 @default.
- W4212778719 cites W2104675226 @default.
- W4212778719 cites W2111993777 @default.
- W4212778719 cites W2113869435 @default.
- W4212778719 cites W2120506831 @default.
- W4212778719 cites W2131117641 @default.
- W4212778719 cites W2140128594 @default.
- W4212778719 cites W2143938161 @default.
- W4212778719 cites W2149729365 @default.
- W4212778719 cites W2169158003 @default.
- W4212778719 cites W2208114687 @default.
- W4212778719 cites W2414346120 @default.
- W4212778719 cites W2969301554 @default.
- W4212778719 cites W3032905932 @default.
- W4212778719 cites W3083425582 @default.
- W4212778719 cites W3146213136 @default.
- W4212778719 cites W3181657358 @default.
- W4212778719 doi "https://doi.org/10.2174/0929867329666220216111952" @default.
- W4212778719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35170405" @default.
- W4212778719 hasPublicationYear "2022" @default.
- W4212778719 type Work @default.
- W4212778719 citedByCount "2" @default.
- W4212778719 countsByYear W42127787192022 @default.
- W4212778719 countsByYear W42127787192023 @default.
- W4212778719 crossrefType "journal-article" @default.
- W4212778719 hasAuthorship W4212778719A5007286842 @default.
- W4212778719 hasAuthorship W4212778719A5016402345 @default.
- W4212778719 hasAuthorship W4212778719A5055426982 @default.
- W4212778719 hasAuthorship W4212778719A5060902570 @default.
- W4212778719 hasAuthorship W4212778719A5063850713 @default.
- W4212778719 hasAuthorship W4212778719A5085329149 @default.
- W4212778719 hasConcept C131667965 @default.
- W4212778719 hasConcept C169760540 @default.
- W4212778719 hasConcept C181199279 @default.
- W4212778719 hasConcept C202033177 @default.
- W4212778719 hasConcept C2777093970 @default.
- W4212778719 hasConcept C2780827179 @default.
- W4212778719 hasConcept C2781040256 @default.
- W4212778719 hasConcept C2781114197 @default.
- W4212778719 hasConcept C55493867 @default.
- W4212778719 hasConcept C62826618 @default.
- W4212778719 hasConcept C86803240 @default.
- W4212778719 hasConcept C95444343 @default.
- W4212778719 hasConceptScore W4212778719C131667965 @default.
- W4212778719 hasConceptScore W4212778719C169760540 @default.
- W4212778719 hasConceptScore W4212778719C181199279 @default.
- W4212778719 hasConceptScore W4212778719C202033177 @default.
- W4212778719 hasConceptScore W4212778719C2777093970 @default.
- W4212778719 hasConceptScore W4212778719C2780827179 @default.
- W4212778719 hasConceptScore W4212778719C2781040256 @default.
- W4212778719 hasConceptScore W4212778719C2781114197 @default.
- W4212778719 hasConceptScore W4212778719C55493867 @default.
- W4212778719 hasConceptScore W4212778719C62826618 @default.
- W4212778719 hasConceptScore W4212778719C86803240 @default.
- W4212778719 hasConceptScore W4212778719C95444343 @default.
- W4212778719 hasFunder F4320321001 @default.