FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4212787553> ?p ?o ?g. }

• W4212787553 endingPage "1183" @default.
• W4212787553 startingPage "1172" @default.
• W4212787553 abstract "Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in combination with CRISPR/Cas9 genome editing provide unparalleled opportunities to study cardiac biology and disease. However, sarcomeres, the fundamental units of myocyte contraction, are immature and nonlinear in hiPSC-CMs, which technically challenge accurate functional interrogation of contractile parameters in beating cells. Furthermore, existing analysis methods are relatively low-throughput, indirectly assess contractility, or only assess well-aligned sarcomeres found in mature cardiac tissues.We aimed to develop an analysis platform that directly, rapidly, and automatically tracks sarcomeres in beating cardiomyocytes. The platform should assess sarcomere content, contraction and relaxation parameters, and beat rate.We developed SarcTrack, a MatLab software that monitors fluorescently tagged sarcomeres in hiPSC-CMs. The algorithm determines sarcomere content, sarcomere length, and returns rates of sarcomere contraction and relaxation. By rapid measurement of hundreds of sarcomeres in each hiPSC-CM, SarcTrack provides large data sets for robust statistical analyses of multiple contractile parameters. We validated SarcTrack by analyzing drug-treated hiPSC-CMs, confirming the contractility effects of compounds that directly activate (CK-1827452) or inhibit (MYK-461) myosin molecules or indirectly alter contractility (verapamil and propranolol). SarcTrack analysis of hiPSC-CMs carrying a heterozygous truncation variant in the myosin-binding protein C ( MYBPC3) gene, which causes hypertrophic cardiomyopathy, recapitulated seminal disease phenotypes including cardiac hypercontractility and diminished relaxation, abnormalities that normalized with MYK-461 treatment.SarcTrack provides a direct and efficient method to quantitatively assess sarcomere function. By improving existing contractility analysis methods and overcoming technical challenges associated with functional evaluation of hiPSC-CMs, SarcTrack enhances translational prospects for sarcomere-regulating therapeutics and accelerates interrogation of human cardiac genetic variants." @default.
• W4212787553 created "2022-02-24" @default.
• W4212787553 creator A5004976391 @default.
• W4212787553 creator A5005091829 @default.
• W4212787553 creator A5009311626 @default.
• W4212787553 creator A5021544196 @default.
• W4212787553 creator A5029778986 @default.
• W4212787553 creator A5035983984 @default.
• W4212787553 creator A5038207590 @default.
• W4212787553 creator A5038484024 @default.
• W4212787553 creator A5047696247 @default.
• W4212787553 creator A5061208512 @default.
• W4212787553 creator A5068603192 @default.
• W4212787553 creator A5070508954 @default.
• W4212787553 creator A5072335242 @default.
• W4212787553 creator A5073934514 @default.
• W4212787553 creator A5074752569 @default.
• W4212787553 creator A5090202792 @default.
• W4212787553 date "2019-04-12" @default.
• W4212787553 modified "2023-10-17" @default.
• W4212787553 title "SarcTrack" @default.
• W4212787553 cites W1953958400 @default.
• W4212787553 cites W2003171404 @default.
• W4212787553 cites W2043702016 @default.
• W4212787553 cites W2062298134 @default.
• W4212787553 cites W2066390373 @default.
• W4212787553 cites W2085950714 @default.
• W4212787553 cites W2088489842 @default.
• W4212787553 cites W2096087960 @default.
• W4212787553 cites W2120795306 @default.
• W4212787553 cites W2133126583 @default.
• W4212787553 cites W2134406150 @default.
• W4212787553 cites W2137802443 @default.
• W4212787553 cites W2146058202 @default.
• W4212787553 cites W2155828174 @default.
• W4212787553 cites W2200325631 @default.
• W4212787553 cites W2260480416 @default.
• W4212787553 cites W2272318571 @default.
• W4212787553 cites W2277697424 @default.
• W4212787553 cites W2465639163 @default.
• W4212787553 cites W2517568305 @default.
• W4212787553 cites W2587938050 @default.
• W4212787553 cites W2751515929 @default.
• W4212787553 cites W2753635270 @default.
• W4212787553 cites W2756203218 @default.
• W4212787553 cites W2773637439 @default.
• W4212787553 cites W2778022289 @default.
• W4212787553 cites W2783266799 @default.
• W4212787553 cites W2794616878 @default.
• W4212787553 cites W2799564816 @default.
• W4212787553 cites W2802665748 @default.
• W4212787553 cites W2894495525 @default.
• W4212787553 cites W2900980188 @default.
• W4212787553 cites W2913785049 @default.
• W4212787553 doi "https://doi.org/10.1161/circresaha.118.314505" @default.
• W4212787553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30700234" @default.
• W4212787553 hasPublicationYear "2019" @default.
• W4212787553 type Work @default.
• W4212787553 citedByCount "86" @default.
• W4212787553 countsByYear W42127875532019 @default.
• W4212787553 countsByYear W42127875532020 @default.
• W4212787553 countsByYear W42127875532021 @default.
• W4212787553 countsByYear W42127875532022 @default.
• W4212787553 countsByYear W42127875532023 @default.
• W4212787553 crossrefType "journal-article" @default.
• W4212787553 hasAuthorship W4212787553A5004976391 @default.
• W4212787553 hasAuthorship W4212787553A5005091829 @default.
• W4212787553 hasAuthorship W4212787553A5009311626 @default.
• W4212787553 hasAuthorship W4212787553A5021544196 @default.
• W4212787553 hasAuthorship W4212787553A5029778986 @default.
• W4212787553 hasAuthorship W4212787553A5035983984 @default.
• W4212787553 hasAuthorship W4212787553A5038207590 @default.
• W4212787553 hasAuthorship W4212787553A5038484024 @default.
• W4212787553 hasAuthorship W4212787553A5047696247 @default.
• W4212787553 hasAuthorship W4212787553A5061208512 @default.
• W4212787553 hasAuthorship W4212787553A5068603192 @default.
• W4212787553 hasAuthorship W4212787553A5070508954 @default.
• W4212787553 hasAuthorship W4212787553A5072335242 @default.
• W4212787553 hasAuthorship W4212787553A5073934514 @default.
• W4212787553 hasAuthorship W4212787553A5074752569 @default.
• W4212787553 hasAuthorship W4212787553A5090202792 @default.
• W4212787553 hasBestOaLocation W42127875531 @default.
• W4212787553 hasConcept C126322002 @default.
• W4212787553 hasConcept C133702464 @default.
• W4212787553 hasConcept C142669718 @default.
• W4212787553 hasConcept C1491633281 @default.
• W4212787553 hasConcept C163415756 @default.
• W4212787553 hasConcept C164705383 @default.
• W4212787553 hasConcept C185592680 @default.
• W4212787553 hasConcept C207200792 @default.
• W4212787553 hasConcept C39133596 @default.
• W4212787553 hasConcept C54355233 @default.
• W4212787553 hasConcept C68731436 @default.
• W4212787553 hasConcept C6997183 @default.
• W4212787553 hasConcept C71924100 @default.
• W4212787553 hasConcept C86803240 @default.
• W4212787553 hasConcept C95444343 @default.
• W4212787553 hasConceptScore W4212787553C126322002 @default.

• next