Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213031621> ?p ?o ?g. }
- W4213031621 endingPage "146333" @default.
- W4213031621 startingPage "146333" @default.
- W4213031621 abstract "Liver cancer is one of the most lethal cancers having worldwide prevalence. Despite significant progress in cancer therapy, liver cancer-induced mortality is very high. Nanog, as an essential transcription factor modulating cellular multipotency, causes tumor progression, drug resistance, and preserves stemness properties in various tumors such as liver cancer. Thus, this research was conducted to evaluate the impact of combination therapy of Nanog siRNA/cisplatin on the sensitivity of liver cancer cells to this drug. HepG2 cells were transfected with Nanog siRNA and treated with cisplatin, individually and in combination. Then, it was observed that in transfected HepG2 cells, Nanog expression was significantly reduced at mRNA level and also these cells were sensitized to cisplatin. In addition, to assess the impact of Nanog siRNA and cisplatin individually and in combination on cells' viability, migration capacity, apoptosis, and cell cycle progression, the MTT, wound healing, colony formation assay, Annexin V/PI staining, and flow cytometry assays were applied on HepG2 cells, respectively. Also, the quantitive Real-Time PCR was used to check the expression of stemness-associated genes (CD44, CD133, and Sox2), and apoptosis-related genes (caspase-3, 8, 9, BAX and Bcl2) after combination therapy. It is indicated that the combination of Nanog siRNA and cisplatin significantly reduced proliferation, migration, and colony formation ability, as well as increased apoptosis rate, and cell cycle arrest. Also, it is found that the combination of Nanog siRNA and cisplatin down-regulated the expression of stemness-associated genes and up-regulated apoptosis-related genes in HepG2 cells. Hence, it can be suggested that Nanog inhibition in combination with cisplatin is a potential therapeutic strategy for developing new therapeutic approaches for liver cancer." @default.
- W4213031621 created "2022-02-24" @default.
- W4213031621 creator A5006117364 @default.
- W4213031621 creator A5009266201 @default.
- W4213031621 creator A5044638009 @default.
- W4213031621 creator A5051457623 @default.
- W4213031621 creator A5061890110 @default.
- W4213031621 creator A5063938536 @default.
- W4213031621 creator A5068419317 @default.
- W4213031621 creator A5081334342 @default.
- W4213031621 date "2022-05-01" @default.
- W4213031621 modified "2023-10-16" @default.
- W4213031621 title "siRNA-mediated silencing of Nanog reduces stemness properties and increases the sensitivity of HepG2 cells to cisplatin" @default.
- W4213031621 cites W145577621 @default.
- W4213031621 cites W1963590109 @default.
- W4213031621 cites W1964903539 @default.
- W4213031621 cites W1965672573 @default.
- W4213031621 cites W1983965827 @default.
- W4213031621 cites W1991354119 @default.
- W4213031621 cites W1999189296 @default.
- W4213031621 cites W2000481222 @default.
- W4213031621 cites W2030183250 @default.
- W4213031621 cites W2031308048 @default.
- W4213031621 cites W2046769573 @default.
- W4213031621 cites W2053946898 @default.
- W4213031621 cites W2056992430 @default.
- W4213031621 cites W2061032400 @default.
- W4213031621 cites W2082823320 @default.
- W4213031621 cites W2088026312 @default.
- W4213031621 cites W2093444984 @default.
- W4213031621 cites W2096843793 @default.
- W4213031621 cites W2101519297 @default.
- W4213031621 cites W2126851387 @default.
- W4213031621 cites W2130177169 @default.
- W4213031621 cites W2150163104 @default.
- W4213031621 cites W2164618754 @default.
- W4213031621 cites W2274595380 @default.
- W4213031621 cites W2318707908 @default.
- W4213031621 cites W2593316971 @default.
- W4213031621 cites W2969802526 @default.
- W4213031621 cites W2996903121 @default.
- W4213031621 cites W3000422793 @default.
- W4213031621 cites W3001385677 @default.
- W4213031621 cites W3003844152 @default.
- W4213031621 cites W3004517392 @default.
- W4213031621 cites W3004742774 @default.
- W4213031621 cites W3005640056 @default.
- W4213031621 cites W3011748021 @default.
- W4213031621 cites W3020785335 @default.
- W4213031621 cites W3024158331 @default.
- W4213031621 cites W3039693922 @default.
- W4213031621 cites W3043794278 @default.
- W4213031621 cites W3044628240 @default.
- W4213031621 cites W3047662296 @default.
- W4213031621 cites W3080173129 @default.
- W4213031621 cites W3087496260 @default.
- W4213031621 cites W3089811751 @default.
- W4213031621 cites W3108384843 @default.
- W4213031621 cites W3113330724 @default.
- W4213031621 cites W3122477600 @default.
- W4213031621 cites W3139294734 @default.
- W4213031621 cites W3140395279 @default.
- W4213031621 cites W3158852481 @default.
- W4213031621 cites W3186617617 @default.
- W4213031621 cites W3207674391 @default.
- W4213031621 cites W3208129051 @default.
- W4213031621 cites W4243625103 @default.
- W4213031621 doi "https://doi.org/10.1016/j.gene.2022.146333" @default.
- W4213031621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35182674" @default.
- W4213031621 hasPublicationYear "2022" @default.
- W4213031621 type Work @default.
- W4213031621 citedByCount "6" @default.
- W4213031621 countsByYear W42130316212022 @default.
- W4213031621 countsByYear W42130316212023 @default.
- W4213031621 crossrefType "journal-article" @default.
- W4213031621 hasAuthorship W4213031621A5006117364 @default.
- W4213031621 hasAuthorship W4213031621A5009266201 @default.
- W4213031621 hasAuthorship W4213031621A5044638009 @default.
- W4213031621 hasAuthorship W4213031621A5051457623 @default.
- W4213031621 hasAuthorship W4213031621A5061890110 @default.
- W4213031621 hasAuthorship W4213031621A5063938536 @default.
- W4213031621 hasAuthorship W4213031621A5068419317 @default.
- W4213031621 hasAuthorship W4213031621A5081334342 @default.
- W4213031621 hasConcept C104317684 @default.
- W4213031621 hasConcept C107459253 @default.
- W4213031621 hasConcept C119056186 @default.
- W4213031621 hasConcept C121608353 @default.
- W4213031621 hasConcept C145103041 @default.
- W4213031621 hasConcept C1491633281 @default.
- W4213031621 hasConcept C153911025 @default.
- W4213031621 hasConcept C190283241 @default.
- W4213031621 hasConcept C207701140 @default.
- W4213031621 hasConcept C22615655 @default.
- W4213031621 hasConcept C2776694085 @default.
- W4213031621 hasConcept C2777933648 @default.
- W4213031621 hasConcept C2778239845 @default.
- W4213031621 hasConcept C2780932548 @default.
- W4213031621 hasConcept C28328180 @default.