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- W4213074447 abstract "Abstract Objective: Transcriptome study of the desired crossbreed and comparing it with the pure parental population is a method for evaluating and selecting cross with appropriate products, yet disease resistance, high tolerance to environmental stresses, and compatibility with the climate of the breeding tract. RNA-Sequencing (RNA-seq) technology was employed to investigate the effect of crossbreeding by the Montbeliard cattle on the “Sistani cattle” transcriptome. Blood samples were collected from 90 cows (45 pure and 45 crossbreeds). Total RNA was extracted in the Media Teb Gene Laboratory of Iran. The mRNA selection, library preparation, and sequencing were performed at BGI Shenzhen, China on an Illumina HiSeq 2500 sequencer. After assembling the transcripts with the bovine reference genome, Cuffdiff was used to detect differential gene expression in purebred and its crossbred. Results: In total, 45 significantly differentially expressed genes were identified (false discovery rate q< 0.05) between purebred Sistani and “Sistani×Montbeliard” crossbreed. These genes are reported to have a role in immune response, calving, fertility, and resistance to mastitis. Besides, they may have a role in different levels of heat tolerance and disease resistance. Therefore, Sistani×Montbeliard crossbred might be suitable for the climatic conditions of the Sistan region in Iran." @default.
- W4213074447 created "2022-02-24" @default.
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- W4213074447 date "2022-02-21" @default.
- W4213074447 modified "2023-10-18" @default.
- W4213074447 title "The effect of crossbreeding by the Montbeliard cattle on the transcriptome of the Sistani cattle" @default.
- W4213074447 doi "https://doi.org/10.21203/rs.3.rs-1253311/v1" @default.
- W4213074447 hasPublicationYear "2022" @default.
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