Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213090784> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W4213090784 endingPage "1662" @default.
- W4213090784 startingPage "1661" @default.
- W4213090784 abstract "Head and neck cancer, which predominantly arises from the oral mucosa, represents the sixth most common malignancy worldwide. These cancer cells can be resistant to programmed cell death triggered by extrinsic stimuli due to innate overexpression of inhibitor of apoptosis proteins (IAPs). The cellular protein second mitochondria-derived activator of caspases (SMAC) can antagonize IAP-induced caspase inhibition and thus trigger apoptosis. Here, we investigate the cell death-sensitizing effects of the SMAC mimetic LCL161 alone and in combination with Fas ligand (FasL) using a panel of six cell lines. Fas receptor (FasR) expression was analyzed by flow cytometry. Cells were treated with FasL and LCL161 alone or in combination, and cytotoxicity was measured using crystal violet assays. Annexin V and cell viability assays using zVAD-fmk and Necrostatin-1 (Nec-1) were carried out to assess the type of programmed cell death induced by LCL161. To demonstrate the sensitizing effects of LCL161, we employed the t-test to compare the effects of FasL alone and in combination with LCL161. Linear regression analysis was performed to determine initial and half maximal inhibitory concentrations (IC10 and IC50, respectively). Distinct FasR expression was detected in each cell line. Four of six cell lines were significantly sensitized to FasL by LCL161 (p < 0.05), and synergistic effects were observed (y < 1). Moreover, the initially resistant cell line SCC-25 was effectively sensitized to FasL by LCL161. Annexin V FACS analysis demonstrated apoptosis-sensitizing and apoptosis-inducing effects of LCL161 across all cell lines. Using specific cell death inhibitors (zVAD-fmk and Nec-1), we demonstrated that LCL161-initiated apoptosis could not be prevented, highlighting the proapoptotic potential of this mimetic in these cells. Our findings show the effectiveness of apoptotic sensitization of OSCC cells by LCL161 in combination with FasL, thus confirming the importance of an IAP-targeting therapeutic approach for oral squamous cell carcinoma." @default.
- W4213090784 created "2022-02-24" @default.
- W4213090784 date "2019-10-01" @default.
- W4213090784 modified "2023-09-27" @default.
- W4213090784 title "EACMFS Prizes & Awards" @default.
- W4213090784 doi "https://doi.org/10.1016/s1010-5182(19)31020-0" @default.
- W4213090784 hasPublicationYear "2019" @default.
- W4213090784 type Work @default.
- W4213090784 citedByCount "0" @default.
- W4213090784 crossrefType "journal-article" @default.
- W4213090784 hasConcept C153911025 @default.
- W4213090784 hasConcept C190283241 @default.
- W4213090784 hasConcept C203014093 @default.
- W4213090784 hasConcept C2777408456 @default.
- W4213090784 hasConcept C31573885 @default.
- W4213090784 hasConcept C33195913 @default.
- W4213090784 hasConcept C502942594 @default.
- W4213090784 hasConcept C54355233 @default.
- W4213090784 hasConcept C553184892 @default.
- W4213090784 hasConcept C55493867 @default.
- W4213090784 hasConcept C66008609 @default.
- W4213090784 hasConcept C71924100 @default.
- W4213090784 hasConcept C81885089 @default.
- W4213090784 hasConcept C86803240 @default.
- W4213090784 hasConcept C88634738 @default.
- W4213090784 hasConcept C98424977 @default.
- W4213090784 hasConceptScore W4213090784C153911025 @default.
- W4213090784 hasConceptScore W4213090784C190283241 @default.
- W4213090784 hasConceptScore W4213090784C203014093 @default.
- W4213090784 hasConceptScore W4213090784C2777408456 @default.
- W4213090784 hasConceptScore W4213090784C31573885 @default.
- W4213090784 hasConceptScore W4213090784C33195913 @default.
- W4213090784 hasConceptScore W4213090784C502942594 @default.
- W4213090784 hasConceptScore W4213090784C54355233 @default.
- W4213090784 hasConceptScore W4213090784C553184892 @default.
- W4213090784 hasConceptScore W4213090784C55493867 @default.
- W4213090784 hasConceptScore W4213090784C66008609 @default.
- W4213090784 hasConceptScore W4213090784C71924100 @default.
- W4213090784 hasConceptScore W4213090784C81885089 @default.
- W4213090784 hasConceptScore W4213090784C86803240 @default.
- W4213090784 hasConceptScore W4213090784C88634738 @default.
- W4213090784 hasConceptScore W4213090784C98424977 @default.
- W4213090784 hasIssue "10" @default.
- W4213090784 hasLocation W42130907841 @default.
- W4213090784 hasOpenAccess W4213090784 @default.
- W4213090784 hasPrimaryLocation W42130907841 @default.
- W4213090784 hasRelatedWork W2000225649 @default.
- W4213090784 hasRelatedWork W2015180152 @default.
- W4213090784 hasRelatedWork W2143652083 @default.
- W4213090784 hasRelatedWork W2315878389 @default.
- W4213090784 hasRelatedWork W2352267387 @default.
- W4213090784 hasRelatedWork W2581748696 @default.
- W4213090784 hasRelatedWork W2914246542 @default.
- W4213090784 hasRelatedWork W2944132818 @default.
- W4213090784 hasRelatedWork W2962955586 @default.
- W4213090784 hasRelatedWork W3029638009 @default.
- W4213090784 hasVolume "47" @default.
- W4213090784 isParatext "false" @default.
- W4213090784 isRetracted "false" @default.
- W4213090784 workType "article" @default.