FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213103418> ?p ?o ?g. }

• W4213103418 abstract "Abstract The remodelling of the epigenome and transcriptome of the fertilised oocyte to establish totipotency in the zygote and developing embryo is one of the most critical processes in mammalian embryogenesis. Zygotic or embryonic genome activation (ZGA, EGA) in the 2-cell embryo in mouse, and the 8-cell embryo in humans, constitutes the first major wave of transcription. Failure to initiate ZGA leads to developmental defects, and contributes to the high attrition rates of human pre-implantation embryos. Due to limitations in cell numbers and experimental tractability, the mechanisms that regulate human embryonic genome activation in the totipotent embryo remain poorly understood. Here we report the discovery of human 8-cell like cells (8CLCs) specifically among naïve embryonic stem cells, but not primed pluripotent cells. 8CLCs express ZGA marker genes such as ZSCAN4, LEUTX and DUXA and their transcriptome closely resembles that of the 8-cell human embryo. 8-cell like cells reactivate 8-cell stage specific transposable elements such as HERVL and MLT2A1 and are characterized by upregulation of the DNA methylation regulator DPPA3 . 8CLCs show reduced SOX2 protein, and can be identified based on expression of the novel ZGA-associated protein markers TPRX1 and H3.Y in vitro . Overexpression of the transcription factor DUX4 . as well as spliceosome inhibition increase ZGA-like transcription and enhance TPRX1+ 8CLCs formation. Excitingly, the in vitro identified 8CLC marker proteins TPRX1 and H3.Y are also expressed in 8-cell human embryos at the time of genome activation and may thus be relevant in vivo . The discovery of 8CLCs provides a unique opportunity to model and manipulate human ZGA-like transcriptional programs in vitro , and might provide critical functional insights into one of the earliest events in human embryogenesis in vivo . Highlights ZGA markers and transposable elements are expressed in 8CLCs among naïve human stem cells The transcription factor DUX4 and spliceosome inhibition induce ZGA-like transcription 8CLC marker proteins TPRX1 and H3.Y are expressed in nuclei of 8-cell human embryos 8CLCs serve as a novel in vitro model for human ZGA" @default.
• W4213103418 created "2022-02-24" @default.
• W4213103418 creator A5002051588 @default.
• W4213103418 creator A5005275806 @default.
• W4213103418 creator A5008565592 @default.
• W4213103418 creator A5023056756 @default.
• W4213103418 creator A5043294031 @default.
• W4213103418 creator A5048228579 @default.
• W4213103418 creator A5063078130 @default.
• W4213103418 creator A5066726255 @default.
• W4213103418 date "2021-10-29" @default.
• W4213103418 modified "2023-10-17" @default.
• W4213103418 title "Modelling human zygotic genome activation in 8C-like cells <i>in vitro</i>" @default.
• W4213103418 cites W1150470952 @default.
• W4213103418 cites W1972377244 @default.
• W4213103418 cites W1975643185 @default.
• W4213103418 cites W2004261465 @default.
• W4213103418 cites W2005808461 @default.
• W4213103418 cites W2007439698 @default.
• W4213103418 cites W2008675991 @default.
• W4213103418 cites W2009771946 @default.
• W4213103418 cites W2010868220 @default.
• W4213103418 cites W2032512845 @default.
• W4213103418 cites W2034923684 @default.
• W4213103418 cites W2045067641 @default.
• W4213103418 cites W2045899482 @default.
• W4213103418 cites W2055570171 @default.
• W4213103418 cites W2063337177 @default.
• W4213103418 cites W2068878293 @default.
• W4213103418 cites W2086277161 @default.
• W4213103418 cites W2086328680 @default.
• W4213103418 cites W2086943348 @default.
• W4213103418 cites W2090935343 @default.
• W4213103418 cites W2104778396 @default.
• W4213103418 cites W2112785601 @default.
• W4213103418 cites W2125336256 @default.
• W4213103418 cites W2130652821 @default.
• W4213103418 cites W2150114997 @default.
• W4213103418 cites W2270975484 @default.
• W4213103418 cites W2288812985 @default.
• W4213103418 cites W2316095770 @default.
• W4213103418 cites W2418531780 @default.
• W4213103418 cites W2466775243 @default.
• W4213103418 cites W2470453245 @default.
• W4213103418 cites W2515884013 @default.
• W4213103418 cites W2517993048 @default.
• W4213103418 cites W2526632574 @default.
• W4213103418 cites W2599832912 @default.
• W4213103418 cites W2611165465 @default.
• W4213103418 cites W2611346822 @default.
• W4213103418 cites W2616922646 @default.
• W4213103418 cites W2766437506 @default.
• W4213103418 cites W2771422081 @default.
• W4213103418 cites W2773077407 @default.
• W4213103418 cites W2809594440 @default.
• W4213103418 cites W2909484275 @default.
• W4213103418 cites W2914912957 @default.
• W4213103418 cites W2931499292 @default.
• W4213103418 cites W2938430282 @default.
• W4213103418 cites W2952949965 @default.
• W4213103418 cites W2957326146 @default.
• W4213103418 cites W2986064990 @default.
• W4213103418 cites W3022044758 @default.
• W4213103418 cites W3038446536 @default.
• W4213103418 cites W3047141442 @default.
• W4213103418 cites W3080775827 @default.
• W4213103418 cites W3131634425 @default.
• W4213103418 cites W3161508292 @default.
• W4213103418 cites W57992559 @default.
• W4213103418 doi "https://doi.org/10.1101/2021.10.28.466259" @default.
• W4213103418 hasPublicationYear "2021" @default.
• W4213103418 type Work @default.
• W4213103418 citedByCount "2" @default.
• W4213103418 countsByYear W42131034182022 @default.
• W4213103418 crossrefType "posted-content" @default.
• W4213103418 hasAuthorship W4213103418A5002051588 @default.
• W4213103418 hasAuthorship W4213103418A5005275806 @default.
• W4213103418 hasAuthorship W4213103418A5008565592 @default.
• W4213103418 hasAuthorship W4213103418A5023056756 @default.
• W4213103418 hasAuthorship W4213103418A5043294031 @default.
• W4213103418 hasAuthorship W4213103418A5048228579 @default.
• W4213103418 hasAuthorship W4213103418A5063078130 @default.
• W4213103418 hasAuthorship W4213103418A5066726255 @default.
• W4213103418 hasBestOaLocation W42131034181 @default.
• W4213103418 hasConcept C104317684 @default.
• W4213103418 hasConcept C107459253 @default.
• W4213103418 hasConcept C145103041 @default.
• W4213103418 hasConcept C150194340 @default.
• W4213103418 hasConcept C162317418 @default.
• W4213103418 hasConcept C17341445 @default.
• W4213103418 hasConcept C196843134 @default.
• W4213103418 hasConcept C2777933648 @default.
• W4213103418 hasConcept C28748434 @default.
• W4213103418 hasConcept C54355233 @default.
• W4213103418 hasConcept C86803240 @default.
• W4213103418 hasConcept C87073359 @default.
• W4213103418 hasConcept C95444343 @default.
• W4213103418 hasConceptScore W4213103418C104317684 @default.
• W4213103418 hasConceptScore W4213103418C107459253 @default.
• W4213103418 hasConceptScore W4213103418C145103041 @default.

• next