Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213107689> ?p ?o ?g. }
- W4213107689 abstract "Intratumor heterogeneity is a major obstacle to effective cancer treatment. Current methods to study intratumor heterogeneity using single-cell RNA sequencing (scRNA-seq) lack information on the spatial organization of cells. While state-of-the art spatial transcriptomics methods capture the spatial distribution, they either lack single cell resolution or have relatively low transcript counts. Here, we introduce spatially annotated single cell sequencing, based on the previously developed functional single cell sequencing (FUNseq) technique, to spatially profile tumor cells with deep scRNA-seq and single cell resolution. Using our approach, we profiled cells located at different distances from the center of a 2D epithelial cell mass. By profiling the cell patch in concentric bands of varying width, we showed that cells at the outermost edge of the patch responded strongest to their local microenvironment, behaved most invasively, and activated the process of epithelial-to-mesenchymal transition (EMT) to migrate to low-confluence areas. We inferred cell-cell communication networks and demonstrated that cells in the outermost ∼10 cell wide band, which we termed the invasive edge, induced similar phenotypic plasticity in neighboring regions. Applying FUNseq to spatially annotate and profile tumor cells enables deep characterization of tumor subpopulations, thereby unraveling the mechanistic basis for intratumor heterogeneity." @default.
- W4213107689 created "2022-02-24" @default.
- W4213107689 creator A5030734068 @default.
- W4213107689 creator A5031202008 @default.
- W4213107689 creator A5052547773 @default.
- W4213107689 creator A5066608492 @default.
- W4213107689 creator A5076454824 @default.
- W4213107689 creator A5085925373 @default.
- W4213107689 creator A5091021741 @default.
- W4213107689 date "2022-02-22" @default.
- W4213107689 modified "2023-10-05" @default.
- W4213107689 title "Spatially Annotated Single Cell Sequencing for Unraveling Intratumor Heterogeneity" @default.
- W4213107689 cites W1818313583 @default.
- W4213107689 cites W1982550220 @default.
- W4213107689 cites W1984326475 @default.
- W4213107689 cites W1992187858 @default.
- W4213107689 cites W2001954506 @default.
- W4213107689 cites W2007553857 @default.
- W4213107689 cites W2008785575 @default.
- W4213107689 cites W2013958001 @default.
- W4213107689 cites W2029131812 @default.
- W4213107689 cites W2030017878 @default.
- W4213107689 cites W2032518018 @default.
- W4213107689 cites W2040458067 @default.
- W4213107689 cites W2041993682 @default.
- W4213107689 cites W2042113277 @default.
- W4213107689 cites W2061743802 @default.
- W4213107689 cites W2076480857 @default.
- W4213107689 cites W2108275054 @default.
- W4213107689 cites W2150575159 @default.
- W4213107689 cites W2158751174 @default.
- W4213107689 cites W2159707944 @default.
- W4213107689 cites W2164153952 @default.
- W4213107689 cites W2169851453 @default.
- W4213107689 cites W2169895148 @default.
- W4213107689 cites W2201306698 @default.
- W4213107689 cites W2214074259 @default.
- W4213107689 cites W2269017219 @default.
- W4213107689 cites W2332292689 @default.
- W4213107689 cites W2342688693 @default.
- W4213107689 cites W2471536144 @default.
- W4213107689 cites W2526262591 @default.
- W4213107689 cites W2538965655 @default.
- W4213107689 cites W2580449060 @default.
- W4213107689 cites W2607166912 @default.
- W4213107689 cites W2739468380 @default.
- W4213107689 cites W2755156122 @default.
- W4213107689 cites W2771542157 @default.
- W4213107689 cites W2798231099 @default.
- W4213107689 cites W2808465642 @default.
- W4213107689 cites W2889326414 @default.
- W4213107689 cites W2900850487 @default.
- W4213107689 cites W2906067435 @default.
- W4213107689 cites W2971378659 @default.
- W4213107689 cites W2972983657 @default.
- W4213107689 cites W2985106189 @default.
- W4213107689 cites W2997280954 @default.
- W4213107689 cites W3007935255 @default.
- W4213107689 cites W3016840787 @default.
- W4213107689 cites W3021400239 @default.
- W4213107689 cites W3102147110 @default.
- W4213107689 cites W3112563877 @default.
- W4213107689 cites W3164692211 @default.
- W4213107689 cites W3165561581 @default.
- W4213107689 cites W3171692328 @default.
- W4213107689 cites W3175752499 @default.
- W4213107689 cites W3206454724 @default.
- W4213107689 cites W4211233744 @default.
- W4213107689 doi "https://doi.org/10.3389/fbioe.2022.829509" @default.
- W4213107689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35273957" @default.
- W4213107689 hasPublicationYear "2022" @default.
- W4213107689 type Work @default.
- W4213107689 citedByCount "5" @default.
- W4213107689 countsByYear W42131076892023 @default.
- W4213107689 crossrefType "journal-article" @default.
- W4213107689 hasAuthorship W4213107689A5030734068 @default.
- W4213107689 hasAuthorship W4213107689A5031202008 @default.
- W4213107689 hasAuthorship W4213107689A5052547773 @default.
- W4213107689 hasAuthorship W4213107689A5066608492 @default.
- W4213107689 hasAuthorship W4213107689A5076454824 @default.
- W4213107689 hasAuthorship W4213107689A5085925373 @default.
- W4213107689 hasAuthorship W4213107689A5091021741 @default.
- W4213107689 hasBestOaLocation W42131076891 @default.
- W4213107689 hasConcept C104317684 @default.
- W4213107689 hasConcept C121608353 @default.
- W4213107689 hasConcept C127716648 @default.
- W4213107689 hasConcept C132917006 @default.
- W4213107689 hasConcept C141231307 @default.
- W4213107689 hasConcept C1491633281 @default.
- W4213107689 hasConcept C150194340 @default.
- W4213107689 hasConcept C162317418 @default.
- W4213107689 hasConcept C16671776 @default.
- W4213107689 hasConcept C174749747 @default.
- W4213107689 hasConcept C2776950831 @default.
- W4213107689 hasConcept C3018028425 @default.
- W4213107689 hasConcept C54355233 @default.
- W4213107689 hasConcept C70721500 @default.
- W4213107689 hasConcept C86803240 @default.
- W4213107689 hasConcept C95444343 @default.
- W4213107689 hasConceptScore W4213107689C104317684 @default.