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- W4213137291 abstract "Simultaneous imaging of multiple microRNAs (miRNAs) in individual living cells is challenging due to the lack of spectrally distinct encoded fluorophores and non-cytotoxic methods. We describe a multiplexed error-robust combinatorial fluorescent label-encoding method, termed fluorophores encoded error-corrected labels (FluoELs), enabling multiplexed miRNA imaging in living cells with error-correcting capability. The FluoELs comprise proportional dual fluorophores for encoding and a constant quantitative single fluorophore for error-corrected quantification. Both are embedded in 260 nm core-shell silica nanoparticles modified with molecular beacon detection probes. The FluoELs are low cytotoxic and could accurately quantify and spatially resolve nine breast-cancer-related miRNAs and evaluate their coordination. The FluoELs enabled a single-cell analysis platform to evaluate miRNA expression profiles and the molecular mechanisms underlying miRNA-associated diseases." @default.
- W4213137291 created "2022-02-24" @default.
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- W4213137291 date "2022-03-14" @default.
- W4213137291 modified "2023-10-16" @default.
- W4213137291 title "Spatially Resolved, Error‐Robust Multiplexed MicroRNA Profiling in Single Living Cells" @default.
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- W4213137291 doi "https://doi.org/10.1002/anie.202116909" @default.
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