Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213150147> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W4213150147 endingPage "132" @default.
- W4213150147 startingPage "129" @default.
- W4213150147 abstract "Gene editing in mammalian zygotes enables us to generate genetically modified animals rapidly and efficiently. In this study, we compare multiple gene targeting strategies in rat zygotes by generating a novel knock-in reporter rat line to visualize the expression pattern of transcription factor AP-2 gamma (Tfap2c). The targeting vector is designed to replace the stop codon of Tfap2c with T2A-tdTomato sequence. We show that the combination of electroporation-mediated transduction of CRISPR/Cas9 components with adeno-associated virus-mediated transduction of the targeting vector is the most efficient in generating the targeted rat line. The Tfap2c-T2A-tdTomato fluorescence reflects the endogenous expression pattern of Tfap2c in preimplantation embryo, germline, placenta, and forebrain during rat embryo development. The reporter line generated here will be a reliable resource for identifying and purifying Tfap2c expressing cells in rats, and the gene targeting strategy we used can be widely applied for generating desired animals." @default.
- W4213150147 created "2022-02-24" @default.
- W4213150147 creator A5007943598 @default.
- W4213150147 creator A5016204078 @default.
- W4213150147 creator A5027939725 @default.
- W4213150147 creator A5055028459 @default.
- W4213150147 creator A5059937255 @default.
- W4213150147 creator A5077051133 @default.
- W4213150147 creator A5078622682 @default.
- W4213150147 creator A5080154032 @default.
- W4213150147 creator A5080622561 @default.
- W4213150147 creator A5081181531 @default.
- W4213150147 creator A5087239539 @default.
- W4213150147 date "2022-02-15" @default.
- W4213150147 modified "2023-10-16" @default.
- W4213150147 title "Generation of Tfap2c‐T2A‐tdTomato knock‐in reporter rats via adeno‐associated virus‐mediated efficient gene targeting" @default.
- W4213150147 cites W2159584345 @default.
- W4213150147 cites W2898974045 @default.
- W4213150147 cites W3003883153 @default.
- W4213150147 doi "https://doi.org/10.1002/mrd.23562" @default.
- W4213150147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35170139" @default.
- W4213150147 hasPublicationYear "2022" @default.
- W4213150147 type Work @default.
- W4213150147 citedByCount "0" @default.
- W4213150147 crossrefType "journal-article" @default.
- W4213150147 hasAuthorship W4213150147A5007943598 @default.
- W4213150147 hasAuthorship W4213150147A5016204078 @default.
- W4213150147 hasAuthorship W4213150147A5027939725 @default.
- W4213150147 hasAuthorship W4213150147A5055028459 @default.
- W4213150147 hasAuthorship W4213150147A5059937255 @default.
- W4213150147 hasAuthorship W4213150147A5077051133 @default.
- W4213150147 hasAuthorship W4213150147A5078622682 @default.
- W4213150147 hasAuthorship W4213150147A5080154032 @default.
- W4213150147 hasAuthorship W4213150147A5080622561 @default.
- W4213150147 hasAuthorship W4213150147A5081181531 @default.
- W4213150147 hasAuthorship W4213150147A5087239539 @default.
- W4213150147 hasConcept C102230213 @default.
- W4213150147 hasConcept C104317684 @default.
- W4213150147 hasConcept C109825262 @default.
- W4213150147 hasConcept C111829913 @default.
- W4213150147 hasConcept C142613039 @default.
- W4213150147 hasConcept C145103041 @default.
- W4213150147 hasConcept C150194340 @default.
- W4213150147 hasConcept C15152581 @default.
- W4213150147 hasConcept C153911025 @default.
- W4213150147 hasConcept C161733203 @default.
- W4213150147 hasConcept C186852380 @default.
- W4213150147 hasConcept C2778107364 @default.
- W4213150147 hasConcept C2796436 @default.
- W4213150147 hasConcept C40767141 @default.
- W4213150147 hasConcept C54355233 @default.
- W4213150147 hasConcept C55493867 @default.
- W4213150147 hasConcept C86803240 @default.
- W4213150147 hasConcept C92087593 @default.
- W4213150147 hasConcept C95444343 @default.
- W4213150147 hasConcept C98108389 @default.
- W4213150147 hasConceptScore W4213150147C102230213 @default.
- W4213150147 hasConceptScore W4213150147C104317684 @default.
- W4213150147 hasConceptScore W4213150147C109825262 @default.
- W4213150147 hasConceptScore W4213150147C111829913 @default.
- W4213150147 hasConceptScore W4213150147C142613039 @default.
- W4213150147 hasConceptScore W4213150147C145103041 @default.
- W4213150147 hasConceptScore W4213150147C150194340 @default.
- W4213150147 hasConceptScore W4213150147C15152581 @default.
- W4213150147 hasConceptScore W4213150147C153911025 @default.
- W4213150147 hasConceptScore W4213150147C161733203 @default.
- W4213150147 hasConceptScore W4213150147C186852380 @default.
- W4213150147 hasConceptScore W4213150147C2778107364 @default.
- W4213150147 hasConceptScore W4213150147C2796436 @default.
- W4213150147 hasConceptScore W4213150147C40767141 @default.
- W4213150147 hasConceptScore W4213150147C54355233 @default.
- W4213150147 hasConceptScore W4213150147C55493867 @default.
- W4213150147 hasConceptScore W4213150147C86803240 @default.
- W4213150147 hasConceptScore W4213150147C92087593 @default.
- W4213150147 hasConceptScore W4213150147C95444343 @default.
- W4213150147 hasConceptScore W4213150147C98108389 @default.
- W4213150147 hasFunder F4320334764 @default.
- W4213150147 hasIssue "3" @default.
- W4213150147 hasLocation W42131501471 @default.
- W4213150147 hasLocation W42131501472 @default.
- W4213150147 hasOpenAccess W4213150147 @default.
- W4213150147 hasPrimaryLocation W42131501471 @default.
- W4213150147 hasRelatedWork W1584060797 @default.
- W4213150147 hasRelatedWork W1594199829 @default.
- W4213150147 hasRelatedWork W2001034508 @default.
- W4213150147 hasRelatedWork W2043166243 @default.
- W4213150147 hasRelatedWork W2108672978 @default.
- W4213150147 hasRelatedWork W2384885086 @default.
- W4213150147 hasRelatedWork W2583459522 @default.
- W4213150147 hasRelatedWork W2620819461 @default.
- W4213150147 hasRelatedWork W2868184695 @default.
- W4213150147 hasRelatedWork W4353084173 @default.
- W4213150147 hasVolume "89" @default.
- W4213150147 isParatext "false" @default.
- W4213150147 isRetracted "false" @default.
- W4213150147 workType "article" @default.