Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213170163> ?p ?o ?g. }
- W4213170163 endingPage "3684" @default.
- W4213170163 startingPage "3677" @default.
- W4213170163 abstract "Nucleic acid analysis has been at the forefront of the COVID-19 global health crisis where millions of diagnostic tests have been used to determine disease status as well as sequencing techniques that monitor the evolving genome of SARS-CoV-2. In this study, we report the development of a sample preparation method that decreases the time required for DNA isolation while significantly increasing the sensitivity of downstream analysis. Functionalized planar supports are modified with a polymeric ionic liquid sorbent coating to form thin film microextraction (TFME) devices. The extraction devices are shown to have a high affinity for DNA while also exhibiting high reproducibility and reusability. Using quantitative polymerase chain reaction (qPCR) analysis, the TFME devices are shown to require low equilibration times while achieving higher preconcentration factors than solid-phase microextraction (SPME) by extracting larger masses of DNA. Rapid desorption kinetics enable higher DNA recoveries using desorption solutions that are less inhibitory to qPCR and loop-mediated isothermal amplification (LAMP). To demonstrate the advantageous features of the TFME platform, a customized leuco crystal violet LAMP assay is used for visual detection of the ORF1ab DNA sequence from SARS-CoV-2 spiked into artificial oral fluid samples. When coupled to the TFME platform, 100% of LAMP reactions were positive for SARS-CoV-2 compared to 66.7% obtained by SPME for a clinically relevant concentration of 4.80 × 106 DNA copies/mL." @default.
- W4213170163 created "2022-02-24" @default.
- W4213170163 creator A5040431821 @default.
- W4213170163 creator A5045954515 @default.
- W4213170163 creator A5070778623 @default.
- W4213170163 date "2022-02-19" @default.
- W4213170163 modified "2023-09-27" @default.
- W4213170163 title "Thin Film Microextraction Enables Rapid Isolation and Recovery of DNA for Downstream Amplification Assays" @default.
- W4213170163 cites W1491378788 @default.
- W4213170163 cites W1586683291 @default.
- W4213170163 cites W1896186489 @default.
- W4213170163 cites W1968863834 @default.
- W4213170163 cites W1971966881 @default.
- W4213170163 cites W1971983395 @default.
- W4213170163 cites W1973207608 @default.
- W4213170163 cites W1980416814 @default.
- W4213170163 cites W1984771004 @default.
- W4213170163 cites W1997925861 @default.
- W4213170163 cites W1999326276 @default.
- W4213170163 cites W2002538915 @default.
- W4213170163 cites W2009650252 @default.
- W4213170163 cites W2014588425 @default.
- W4213170163 cites W2022562598 @default.
- W4213170163 cites W2023465474 @default.
- W4213170163 cites W2026755253 @default.
- W4213170163 cites W2032978403 @default.
- W4213170163 cites W2038181471 @default.
- W4213170163 cites W2053589876 @default.
- W4213170163 cites W2062128947 @default.
- W4213170163 cites W2063070426 @default.
- W4213170163 cites W2066385972 @default.
- W4213170163 cites W2071857980 @default.
- W4213170163 cites W2082961763 @default.
- W4213170163 cites W2104607395 @default.
- W4213170163 cites W2105275554 @default.
- W4213170163 cites W2124858792 @default.
- W4213170163 cites W2137396156 @default.
- W4213170163 cites W2166229810 @default.
- W4213170163 cites W2167949866 @default.
- W4213170163 cites W2223753513 @default.
- W4213170163 cites W2428588462 @default.
- W4213170163 cites W2463340475 @default.
- W4213170163 cites W2524370159 @default.
- W4213170163 cites W2525148424 @default.
- W4213170163 cites W2586344937 @default.
- W4213170163 cites W2594972835 @default.
- W4213170163 cites W2753668343 @default.
- W4213170163 cites W2767489832 @default.
- W4213170163 cites W2783623954 @default.
- W4213170163 cites W2804686400 @default.
- W4213170163 cites W2806708349 @default.
- W4213170163 cites W2945509230 @default.
- W4213170163 cites W2999723416 @default.
- W4213170163 cites W3020730970 @default.
- W4213170163 cites W3044120595 @default.
- W4213170163 cites W3044938468 @default.
- W4213170163 cites W3093690976 @default.
- W4213170163 cites W3130493281 @default.
- W4213170163 cites W3146376539 @default.
- W4213170163 cites W3148467289 @default.
- W4213170163 cites W3159329565 @default.
- W4213170163 cites W3199592586 @default.
- W4213170163 cites W89741002 @default.
- W4213170163 doi "https://doi.org/10.1021/acs.analchem.1c05380" @default.
- W4213170163 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35184550" @default.
- W4213170163 hasPublicationYear "2022" @default.
- W4213170163 type Work @default.
- W4213170163 citedByCount "8" @default.
- W4213170163 countsByYear W42131701632022 @default.
- W4213170163 countsByYear W42131701632023 @default.
- W4213170163 crossrefType "journal-article" @default.
- W4213170163 hasAuthorship W4213170163A5040431821 @default.
- W4213170163 hasAuthorship W4213170163A5045954515 @default.
- W4213170163 hasAuthorship W4213170163A5070778623 @default.
- W4213170163 hasConcept C104317684 @default.
- W4213170163 hasConcept C150394285 @default.
- W4213170163 hasConcept C162356407 @default.
- W4213170163 hasConcept C162711632 @default.
- W4213170163 hasConcept C178790620 @default.
- W4213170163 hasConcept C185592680 @default.
- W4213170163 hasConcept C205345274 @default.
- W4213170163 hasConcept C24107716 @default.
- W4213170163 hasConcept C2777899863 @default.
- W4213170163 hasConcept C2778533135 @default.
- W4213170163 hasConcept C43617362 @default.
- W4213170163 hasConcept C4725764 @default.
- W4213170163 hasConcept C49105822 @default.
- W4213170163 hasConcept C552990157 @default.
- W4213170163 hasConcept C55493867 @default.
- W4213170163 hasConcept C60635243 @default.
- W4213170163 hasConcept C78063203 @default.
- W4213170163 hasConcept C81426234 @default.
- W4213170163 hasConceptScore W4213170163C104317684 @default.
- W4213170163 hasConceptScore W4213170163C150394285 @default.
- W4213170163 hasConceptScore W4213170163C162356407 @default.
- W4213170163 hasConceptScore W4213170163C162711632 @default.
- W4213170163 hasConceptScore W4213170163C178790620 @default.
- W4213170163 hasConceptScore W4213170163C185592680 @default.