FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213202110> ?p ?o ?g. }

• W4213202110 abstract "Several strategies are used by Escherichia coli to evade the host innate immune system in the blood, such as the cleavage of complement system proteins by secreted proteases. Members of the Serine Proteases Autotransporters of Enterobacteriaceae (SPATE) family have been described as presenting proteolytic effects against complement proteins. Among the SPATE-encoding genes sat (secreted autotransporter toxin) has been detected in high frequencies among strains of E. coli isolated from bacteremia. Sat has been characterized for its cytotoxic action, but the possible immunomodulatory effects of Sat have not been investigated. Therefore, this study aimed to evaluate the proteolytic effects of Sat on complement proteins and the role in pathogenesis of BSI caused by extraintestinal E. coli (ExPEC). E. coli EC071 was selected as a Sat-producing ExPEC strain. Whole-genome sequencing showed that sat sequences of EC071 and uropathogenic E. coli CFT073 present 99% identity. EC071 was shown to be resistant to the bactericidal activity of normal human serum (NHS). Purified native Sat was used in proteolytic assays with proteins of the complement system and, except for C1q, all tested substrates were cleaved by Sat in a dose and time-dependent manner. Moreover, E. coli DH5α survived in NHS pre-incubated with Sat. EC071-derivative strains harboring sat knockout and in trans complementations producing either active or non-active Sat were tested in a murine sepsis model. Lethality was reduced by 50% when mice were inoculated with the sat mutant strain. The complemented strain producing active Sat partially restored the effect caused by the wild-type strain. The results presented in this study show that Sat presents immunomodulatory effects by cleaving several proteins of the three complement system pathways. Therefore, Sat plays an important role in the establishment of bloodstream infections and sepsis." @default.
• W4213202110 created "2022-02-24" @default.
• W4213202110 creator A5015762799 @default.
• W4213202110 creator A5030866254 @default.
• W4213202110 creator A5032550179 @default.
• W4213202110 creator A5034695637 @default.
• W4213202110 creator A5042873876 @default.
• W4213202110 creator A5046670021 @default.
• W4213202110 creator A5064343259 @default.
• W4213202110 creator A5069970039 @default.
• W4213202110 date "2022-02-17" @default.
• W4213202110 modified "2023-10-01" @default.
• W4213202110 title "Secreted Autotransporter Toxin (Sat) Mediates Innate Immune System Evasion" @default.
• W4213202110 cites W142478193 @default.
• W4213202110 cites W1486365785 @default.
• W4213202110 cites W1499593166 @default.
• W4213202110 cites W1878365783 @default.
• W4213202110 cites W1897068684 @default.
• W4213202110 cites W1972704410 @default.
• W4213202110 cites W1974373204 @default.
• W4213202110 cites W1982563191 @default.
• W4213202110 cites W1989814427 @default.
• W4213202110 cites W1992708993 @default.
• W4213202110 cites W1994634407 @default.
• W4213202110 cites W1997438069 @default.
• W4213202110 cites W2000282637 @default.
• W4213202110 cites W2018289835 @default.
• W4213202110 cites W2019410656 @default.
• W4213202110 cites W2020963636 @default.
• W4213202110 cites W2021519227 @default.
• W4213202110 cites W2034035871 @default.
• W4213202110 cites W2050555146 @default.
• W4213202110 cites W2054972265 @default.
• W4213202110 cites W2055798634 @default.
• W4213202110 cites W2065196752 @default.
• W4213202110 cites W2069098256 @default.
• W4213202110 cites W2071657131 @default.
• W4213202110 cites W2080840270 @default.
• W4213202110 cites W2089301588 @default.
• W4213202110 cites W2091591930 @default.
• W4213202110 cites W2092598128 @default.
• W4213202110 cites W2094448747 @default.
• W4213202110 cites W2095802793 @default.
• W4213202110 cites W2096093282 @default.
• W4213202110 cites W2097005840 @default.
• W4213202110 cites W2100837269 @default.
• W4213202110 cites W2101629470 @default.
• W4213202110 cites W2107772251 @default.
• W4213202110 cites W2111069670 @default.
• W4213202110 cites W2111216098 @default.
• W4213202110 cites W2114403692 @default.
• W4213202110 cites W2116279310 @default.
• W4213202110 cites W2116984251 @default.
• W4213202110 cites W2125201618 @default.
• W4213202110 cites W2129566418 @default.
• W4213202110 cites W2131271579 @default.
• W4213202110 cites W2138956963 @default.
• W4213202110 cites W2140610365 @default.
• W4213202110 cites W2149163736 @default.
• W4213202110 cites W2150581974 @default.
• W4213202110 cites W2153021084 @default.
• W4213202110 cites W2155208387 @default.
• W4213202110 cites W2156749206 @default.
• W4213202110 cites W2163351388 @default.
• W4213202110 cites W2163517259 @default.
• W4213202110 cites W2163748125 @default.
• W4213202110 cites W2165192857 @default.
• W4213202110 cites W2168933710 @default.
• W4213202110 cites W2170495569 @default.
• W4213202110 cites W2171122216 @default.
• W4213202110 cites W2172286252 @default.
• W4213202110 cites W2221138276 @default.
• W4213202110 cites W2313086788 @default.
• W4213202110 cites W2341787842 @default.
• W4213202110 cites W2342205884 @default.
• W4213202110 cites W2520410321 @default.
• W4213202110 cites W2563433675 @default.
• W4213202110 cites W2586078640 @default.
• W4213202110 cites W2591992124 @default.
• W4213202110 cites W2608823051 @default.
• W4213202110 cites W2608917888 @default.
• W4213202110 cites W2618906471 @default.
• W4213202110 cites W2624129866 @default.
• W4213202110 cites W2651782939 @default.
• W4213202110 cites W2755254747 @default.
• W4213202110 cites W2796914326 @default.
• W4213202110 cites W2809619040 @default.
• W4213202110 cites W2885290913 @default.
• W4213202110 cites W2917323591 @default.
• W4213202110 cites W2946779541 @default.
• W4213202110 cites W2950303887 @default.
• W4213202110 cites W2952672626 @default.
• W4213202110 cites W2989563961 @default.
• W4213202110 cites W3001845850 @default.
• W4213202110 cites W3006793062 @default.
• W4213202110 cites W3006845500 @default.
• W4213202110 cites W3012008443 @default.
• W4213202110 cites W3014385383 @default.
• W4213202110 cites W3115858291 @default.
• W4213202110 cites W3207303255 @default.

• next