FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213202332> ?p ?o ?g. }

• W4213202332 abstract "Ovine and caprine brucellosis, both caused by Brucella melitensis, lead to substantial economic losses in the animal industry and health problems in human populations. Brucella suis strain 2 (B.suis S2), as a live attenuated vaccine, is used extensively in China to prevent brucellosis. It has been proven that microRNA (miRNAs) are involved in the immunopathogenesis of brucellosis; however, the miRNA-driven mechanism of immune response to B.suis S2 in vivo remains unknown. To determine which new miRNAs are involved in the host immune response to B.suis S2 and elucidate the function of these miRNAs, we performed a comprehensive analysis of miRNA expression profiles in sheep immunized with B.suis S2 using the high-throughput sequencing approach. The submandibular lymphatic nodes from sheep seropositive for Brucella were collected at 7, 14, 21, 30, 60 and 90 days post-immunization. MiRNA sequencing analysis revealed that 282 differentially expressed miRNAs (|log2 fold-change |>0.5 and p < 0.05) were significantly enriched in the immune pathways, including the NF-kappa B signaling pathway, B cell receptor signaling pathway, p53 signaling pathway and complement and coagulation cascades. Increasing the threshold to |log2 fold change|>1 and p < 0.01 revealed 48 differentially expressed miRNAs, 31 of which were novel miRNAs. Thirteen of these novel miRNAs, which were differentially expressed for at least two time points, were detected via RT-qPCR assays. The novel_229, novel_609, novel_973 and oar-miR-181a assessed by RT-qPCR were detectable and consistent with the expression patterns obtained by miRNA sequencing. Functional analyses of these miRNAs demonstrated that their target genes participated in the immune response pathways, including the innate and adaptive immunity pathways. The immune-related target genes of novel_229 included ENSOARG00000000649 and TMED1, as well as LCN2, PDPK1 and LPO were novel_609 target genes. The immune-related target genes of novel_973 included C6orf58, SPPL3, BPIFB1, ENSOARG00000021083, MPTX1, CCL28, FGB, IDO1, OLR1 and ENSOARG00000020393. The immune-related target genes of oar-miR-181a included ENSOARG00000002722, ARHGEF2, MFAP4 and DOK2. These results will deepen our understanding of the host miRNA-driven defense mechanism in sheep immunized with B.suis S2 vaccine, and provide the valuable information for optimizing vaccines and developing molecular diagnostic targets." @default.
• W4213202332 created "2022-02-24" @default.
• W4213202332 creator A5001631794 @default.
• W4213202332 creator A5002521285 @default.
• W4213202332 creator A5002971848 @default.
• W4213202332 creator A5006199449 @default.
• W4213202332 creator A5017053622 @default.
• W4213202332 creator A5024113299 @default.
• W4213202332 creator A5025156587 @default.
• W4213202332 creator A5026178343 @default.
• W4213202332 creator A5032344899 @default.
• W4213202332 creator A5034980717 @default.
• W4213202332 creator A5036995681 @default.
• W4213202332 creator A5040155601 @default.
• W4213202332 creator A5040928123 @default.
• W4213202332 creator A5053002387 @default.
• W4213202332 creator A5055420452 @default.
• W4213202332 creator A5056302980 @default.
• W4213202332 creator A5066104113 @default.
• W4213202332 creator A5076571444 @default.
• W4213202332 creator A5085961973 @default.
• W4213202332 date "2022-02-22" @default.
• W4213202332 modified "2023-10-06" @default.
• W4213202332 title "Downregulation of Three Novel miRNAs in the Lymph Nodes of Sheep Immunized With the Brucella suis Strain 2 Vaccine" @default.
• W4213202332 cites W1589692813 @default.
• W4213202332 cites W1996688443 @default.
• W4213202332 cites W2029884260 @default.
• W4213202332 cites W2033942750 @default.
• W4213202332 cites W2059382687 @default.
• W4213202332 cites W2086828658 @default.
• W4213202332 cites W2090801464 @default.
• W4213202332 cites W2096884048 @default.
• W4213202332 cites W2105106534 @default.
• W4213202332 cites W2105679485 @default.
• W4213202332 cites W2107060225 @default.
• W4213202332 cites W2114570899 @default.
• W4213202332 cites W2114700025 @default.
• W4213202332 cites W2123060905 @default.
• W4213202332 cites W2124985265 @default.
• W4213202332 cites W2130305733 @default.
• W4213202332 cites W2160118720 @default.
• W4213202332 cites W2160546144 @default.
• W4213202332 cites W2259061256 @default.
• W4213202332 cites W2264640671 @default.
• W4213202332 cites W2501698907 @default.
• W4213202332 cites W2522812220 @default.
• W4213202332 cites W2652728872 @default.
• W4213202332 cites W2743228635 @default.
• W4213202332 cites W2753345042 @default.
• W4213202332 cites W2792000953 @default.
• W4213202332 cites W2796171479 @default.
• W4213202332 cites W2806440359 @default.
• W4213202332 cites W2885347668 @default.
• W4213202332 cites W2906923134 @default.
• W4213202332 cites W2964788436 @default.
• W4213202332 cites W2984647559 @default.
• W4213202332 cites W3001184014 @default.
• W4213202332 cites W3005704914 @default.
• W4213202332 cites W3026591819 @default.
• W4213202332 cites W3034852029 @default.
• W4213202332 cites W3035563080 @default.
• W4213202332 cites W3080459005 @default.
• W4213202332 cites W3092413061 @default.
• W4213202332 cites W3097780736 @default.
• W4213202332 cites W3127573132 @default.
• W4213202332 cites W3133652100 @default.
• W4213202332 cites W3134241234 @default.
• W4213202332 cites W3135166865 @default.
• W4213202332 cites W3158984054 @default.
• W4213202332 cites W3175932072 @default.
• W4213202332 cites W3176868492 @default.
• W4213202332 cites W3182705332 @default.
• W4213202332 cites W3195835706 @default.
• W4213202332 cites W3198389116 @default.
• W4213202332 cites W4211033510 @default.
• W4213202332 doi "https://doi.org/10.3389/fvets.2022.813170" @default.
• W4213202332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35274021" @default.
• W4213202332 hasPublicationYear "2022" @default.
• W4213202332 type Work @default.
• W4213202332 citedByCount "4" @default.
• W4213202332 countsByYear W42132023322022 @default.
• W4213202332 countsByYear W42132023322023 @default.
• W4213202332 crossrefType "journal-article" @default.
• W4213202332 hasAuthorship W4213202332A5001631794 @default.
• W4213202332 hasAuthorship W4213202332A5002521285 @default.
• W4213202332 hasAuthorship W4213202332A5002971848 @default.
• W4213202332 hasAuthorship W4213202332A5006199449 @default.
• W4213202332 hasAuthorship W4213202332A5017053622 @default.
• W4213202332 hasAuthorship W4213202332A5024113299 @default.
• W4213202332 hasAuthorship W4213202332A5025156587 @default.
• W4213202332 hasAuthorship W4213202332A5026178343 @default.
• W4213202332 hasAuthorship W4213202332A5032344899 @default.
• W4213202332 hasAuthorship W4213202332A5034980717 @default.
• W4213202332 hasAuthorship W4213202332A5036995681 @default.
• W4213202332 hasAuthorship W4213202332A5040155601 @default.
• W4213202332 hasAuthorship W4213202332A5040928123 @default.
• W4213202332 hasAuthorship W4213202332A5053002387 @default.
• W4213202332 hasAuthorship W4213202332A5055420452 @default.
• W4213202332 hasAuthorship W4213202332A5056302980 @default.
• W4213202332 hasAuthorship W4213202332A5066104113 @default.

• next