Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213284312> ?p ?o ?g. }
- W4213284312 endingPage "3636" @default.
- W4213284312 startingPage "3625" @default.
- W4213284312 abstract "Abstract In plants with C3 photosynthesis, increasing the diffusion conductance for CO2 from the substomatal cavity to chloroplast stroma (mesophyll conductance) can improve the efficiencies of both CO2 assimilation and photosynthetic water use. In the diffusion pathway from substomatal cavity to chloroplast stroma, the plasmalemma and chloroplast envelope membranes impose a considerable barrier to CO2 diffusion, limiting photosynthetic efficiency. In an attempt to improve membrane permeability to CO2, and increase photosynthesis in tobacco, we generated transgenic lines in Nicotiana tabacum L. cv Petite Havana carrying either the Arabidopsis PIP1;2 (AtPIP1;2) or PIP1;4 (AtPIP1;4) gene driven by the constitutive dual 2x35S CMV promoter. From a collection of independent T0 transgenics, two T2 lines from each gene were characterized, with western blots confirming increased total aquaporin protein abundance in the AtPIP1;2 tobacco lines. Transient expression of AtPIP1;2-mGFP6 and AtPIP1;4-mGFP6 fusions in Nicotiana benthamiana identified that both AtPIP1;2 and AtPIP1;4 localize to the plasmalemma. Despite achieving ectopic production and correct localization, gas exchange measurements combined with carbon isotope discrimination measurements detected no increase in mesophyll conductance or CO2 assimilation rate in the tobacco lines expressing AtPIP. We discuss the complexities associated with trying to enhance gm through modified aquaporin activity." @default.
- W4213284312 created "2022-02-24" @default.
- W4213284312 creator A5007778944 @default.
- W4213284312 creator A5012467690 @default.
- W4213284312 creator A5018255019 @default.
- W4213284312 creator A5040359852 @default.
- W4213284312 creator A5050531576 @default.
- W4213284312 creator A5063175542 @default.
- W4213284312 date "2022-02-20" @default.
- W4213284312 modified "2023-10-03" @default.
- W4213284312 title "Mesophyll conductance is unaffected by expression of Arabidopsis <i>PIP1</i> aquaporins in the plasmalemma of <i>Nicotiana</i>" @default.
- W4213284312 cites W1593103245 @default.
- W4213284312 cites W1778658114 @default.
- W4213284312 cites W1845342315 @default.
- W4213284312 cites W1944808610 @default.
- W4213284312 cites W1964370652 @default.
- W4213284312 cites W1965927041 @default.
- W4213284312 cites W1994470176 @default.
- W4213284312 cites W2009609492 @default.
- W4213284312 cites W2043689640 @default.
- W4213284312 cites W2045321571 @default.
- W4213284312 cites W2053693922 @default.
- W4213284312 cites W2062134891 @default.
- W4213284312 cites W2062273751 @default.
- W4213284312 cites W2063678126 @default.
- W4213284312 cites W2068234433 @default.
- W4213284312 cites W2072680951 @default.
- W4213284312 cites W2081204547 @default.
- W4213284312 cites W2087665524 @default.
- W4213284312 cites W2107851883 @default.
- W4213284312 cites W2109632095 @default.
- W4213284312 cites W2109975826 @default.
- W4213284312 cites W2113389258 @default.
- W4213284312 cites W2114891756 @default.
- W4213284312 cites W2116459611 @default.
- W4213284312 cites W2118061859 @default.
- W4213284312 cites W2124011928 @default.
- W4213284312 cites W2127022615 @default.
- W4213284312 cites W2133025748 @default.
- W4213284312 cites W2136724762 @default.
- W4213284312 cites W2138696096 @default.
- W4213284312 cites W2139165023 @default.
- W4213284312 cites W2140411722 @default.
- W4213284312 cites W2140915119 @default.
- W4213284312 cites W2144798903 @default.
- W4213284312 cites W2147544081 @default.
- W4213284312 cites W2155016538 @default.
- W4213284312 cites W2156488682 @default.
- W4213284312 cites W2157366717 @default.
- W4213284312 cites W2163448850 @default.
- W4213284312 cites W2212237386 @default.
- W4213284312 cites W2285972467 @default.
- W4213284312 cites W2304006316 @default.
- W4213284312 cites W2537107312 @default.
- W4213284312 cites W2586049680 @default.
- W4213284312 cites W2586143954 @default.
- W4213284312 cites W2625989642 @default.
- W4213284312 cites W2766202801 @default.
- W4213284312 cites W2854316225 @default.
- W4213284312 cites W2903596296 @default.
- W4213284312 cites W2985969722 @default.
- W4213284312 cites W2988596226 @default.
- W4213284312 cites W2994723456 @default.
- W4213284312 cites W2995016723 @default.
- W4213284312 cites W3033045391 @default.
- W4213284312 cites W3043058055 @default.
- W4213284312 cites W3097486414 @default.
- W4213284312 cites W3126842523 @default.
- W4213284312 cites W3130946519 @default.
- W4213284312 cites W3136638862 @default.
- W4213284312 cites W3162967727 @default.
- W4213284312 cites W3174709017 @default.
- W4213284312 cites W4225507140 @default.
- W4213284312 doi "https://doi.org/10.1093/jxb/erac065" @default.
- W4213284312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35184158" @default.
- W4213284312 hasPublicationYear "2022" @default.
- W4213284312 type Work @default.
- W4213284312 citedByCount "7" @default.
- W4213284312 countsByYear W42132843122022 @default.
- W4213284312 countsByYear W42132843122023 @default.
- W4213284312 crossrefType "journal-article" @default.
- W4213284312 hasAuthorship W4213284312A5007778944 @default.
- W4213284312 hasAuthorship W4213284312A5012467690 @default.
- W4213284312 hasAuthorship W4213284312A5018255019 @default.
- W4213284312 hasAuthorship W4213284312A5040359852 @default.
- W4213284312 hasAuthorship W4213284312A5050531576 @default.
- W4213284312 hasAuthorship W4213284312A5063175542 @default.
- W4213284312 hasBestOaLocation W42132843122 @default.
- W4213284312 hasConcept C101692577 @default.
- W4213284312 hasConcept C104317684 @default.
- W4213284312 hasConcept C12554922 @default.
- W4213284312 hasConcept C154280991 @default.
- W4213284312 hasConcept C183688256 @default.
- W4213284312 hasConcept C2780562374 @default.
- W4213284312 hasConcept C2781045423 @default.
- W4213284312 hasConcept C2781205006 @default.
- W4213284312 hasConcept C2911206396 @default.
- W4213284312 hasConcept C55493867 @default.