FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4213302907> ?p ?o ?g. }

• W4213302907 endingPage "115059" @default.
• W4213302907 startingPage "115059" @default.
• W4213302907 abstract "Thamnolia vermicularis (Sw.) Schaer (T. vermicularis) is known to have therapeutic effects on various diseases in Southwest China. Recent research has highlighted that T. vermicularis may suppress Aβ level and Tau hyperphosphorylation to improve the pathological characteristics of Alzheimer's disease, indicating that it might have the potential to treat Alzheimer's disease.The objective of this study was to evaluate the inhibitory effect of T. vermicularis on the fibril formation of a typical amyloidogenic protein, hen egg white lysozyme (HEWL), and to identify the effective components that could potentially enable an extract of T. vermicularis to be used in the development of novel therapeutic agents.A water extract was prepared from T. vermicularis (TVWE) and its inhibitory effect on amyloid fibrillation in vitro was investigated using thioflavin T and 8-anilinonapthalene-1-sulfonic acid spectrofluorometric analyses. The anti-amyloidogenic components of TVWE were separated and qualitatively analyzed using thin layer chromatography (TLC), supercritical carbon dioxide extraction (SFE-CO2), and liquid chromatography-mass spectrometry. Finally, the effect of the bioactive components on the structure of HEWL in the early stages of fibrillogenesis was determined by molecular docking simulation.TVWE strongly inhibited the ability of HEWL to form an amyloid fibril, yielding an IC50 of 0.018 mg/mL for the inhibition of fibrillogenesis. The chemical constituents in the various TVWE fractions resolved by TLC were qualitatively identified by liquid chromatography-quadrupole/time-of-flight mass spectrometry (LC-Q-TOF-MS). The target components were predicted by reviewing the existing literature on T. vermicularis, in which the components of T. vermicularis, along with three small molecules (molecular weight: 182) were preliminarily identified. Molecular docking simulation showed that these small molecules were bound to the core region of HEWL, affecting its stability. Finally, the active anti-amyloidogenic components were extracted from whole T. vermicularis using SFE-CO2 and then identified.The potential components of TVWE that could prevent HEWL fibrillogenesis were primarily identified using TLC, LC-Q-TOF-MS, and SFE-CO2. The candidate small-molecule compounds were further predicted by combining the LC-Q-TOF-MS results with molecular docking analysis. The effective components of T. vermicularis were extracted using SFE-CO2. Together, these methods could constitute a practical strategy for the isolation and identification of anti-amyloidogenic components from a traditional Chinese medicine." @default.
• W4213302907 created "2022-02-24" @default.
• W4213302907 creator A5002451272 @default.
• W4213302907 creator A5006514997 @default.
• W4213302907 creator A5025217307 @default.
• W4213302907 creator A5050471394 @default.
• W4213302907 creator A5056385709 @default.
• W4213302907 creator A5065751214 @default.
• W4213302907 creator A5070787203 @default.
• W4213302907 creator A5088344567 @default.
• W4213302907 creator A5090391649 @default.
• W4213302907 creator A5090678260 @default.
• W4213302907 date "2022-05-01" @default.
• W4213302907 modified "2023-10-14" @default.
• W4213302907 title "Investigation of the chemical structure of anti-amyloidogenic constituents extracted from Thamnolia vermicularis" @default.
• W4213302907 cites W1968788242 @default.
• W4213302907 cites W2007157911 @default.
• W4213302907 cites W2016225317 @default.
• W4213302907 cites W2019482822 @default.
• W4213302907 cites W2042485050 @default.
• W4213302907 cites W2052156860 @default.
• W4213302907 cites W2082196170 @default.
• W4213302907 cites W2174208984 @default.
• W4213302907 cites W2328878803 @default.
• W4213302907 cites W2332298796 @default.
• W4213302907 cites W2527838725 @default.
• W4213302907 cites W2739628150 @default.
• W4213302907 cites W2993936742 @default.
• W4213302907 cites W3005262477 @default.
• W4213302907 cites W3100457457 @default.
• W4213302907 cites W3114639346 @default.
• W4213302907 cites W3134042227 @default.
• W4213302907 cites W3150993279 @default.
• W4213302907 cites W40771570 @default.
• W4213302907 doi "https://doi.org/10.1016/j.jep.2022.115059" @default.
• W4213302907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35114341" @default.
• W4213302907 hasPublicationYear "2022" @default.
• W4213302907 type Work @default.
• W4213302907 citedByCount "0" @default.
• W4213302907 crossrefType "journal-article" @default.
• W4213302907 hasAuthorship W4213302907A5002451272 @default.
• W4213302907 hasAuthorship W4213302907A5006514997 @default.
• W4213302907 hasAuthorship W4213302907A5025217307 @default.
• W4213302907 hasAuthorship W4213302907A5050471394 @default.
• W4213302907 hasAuthorship W4213302907A5056385709 @default.
• W4213302907 hasAuthorship W4213302907A5065751214 @default.
• W4213302907 hasAuthorship W4213302907A5070787203 @default.
• W4213302907 hasAuthorship W4213302907A5088344567 @default.
• W4213302907 hasAuthorship W4213302907A5090391649 @default.
• W4213302907 hasAuthorship W4213302907A5090678260 @default.
• W4213302907 hasConcept C147259501 @default.
• W4213302907 hasConcept C185592680 @default.
• W4213302907 hasConcept C190196608 @default.
• W4213302907 hasConcept C27523624 @default.
• W4213302907 hasConcept C2776966407 @default.
• W4213302907 hasConcept C43617362 @default.
• W4213302907 hasConcept C55493867 @default.
• W4213302907 hasConceptScore W4213302907C147259501 @default.
• W4213302907 hasConceptScore W4213302907C185592680 @default.
• W4213302907 hasConceptScore W4213302907C190196608 @default.
• W4213302907 hasConceptScore W4213302907C27523624 @default.
• W4213302907 hasConceptScore W4213302907C2776966407 @default.
• W4213302907 hasConceptScore W4213302907C43617362 @default.
• W4213302907 hasConceptScore W4213302907C55493867 @default.
• W4213302907 hasFunder F4320321001 @default.
• W4213302907 hasLocation W42133029071 @default.
• W4213302907 hasLocation W42133029072 @default.
• W4213302907 hasOpenAccess W4213302907 @default.
• W4213302907 hasPrimaryLocation W42133029071 @default.
• W4213302907 hasRelatedWork W1564103972 @default.
• W4213302907 hasRelatedWork W1995151343 @default.
• W4213302907 hasRelatedWork W2027293529 @default.
• W4213302907 hasRelatedWork W2085377612 @default.
• W4213302907 hasRelatedWork W2529964895 @default.
• W4213302907 hasRelatedWork W2762993182 @default.
• W4213302907 hasRelatedWork W2788596691 @default.
• W4213302907 hasRelatedWork W2885743982 @default.
• W4213302907 hasRelatedWork W2914891799 @default.
• W4213302907 hasRelatedWork W4280639962 @default.
• W4213302907 hasVolume "289" @default.
• W4213302907 isParatext "false" @default.
• W4213302907 isRetracted "false" @default.
• W4213302907 workType "article" @default.