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- W4213338045 abstract "Enzyme-linked immunosorbent assay (ELISA) is widely used in drug development, disease diagnosis and other fields. Here we report a protein detection method that combines fluorescent-encoded micromagnetic beads and micro holes array technology, which can specifically detect the content of interleukin-6 (IL-6) at the level of pg/mL. In the experiment, permanent magnet was used to increase the falling hole rate of magnetic beads to improve the detection accuracy of digital ELISA, and used COMSOL software to simulate the magnetic field density. The capture magnetic beads combine with IL-6 and the enzyme-labeled complex to form a sandwich structure. The magnetic beads specifically bound to the enzyme label can catalyze the fluorescent substrate to produce fluorescent substances. Then use the image processing program to analyze the fluorescence intensity. The levels of IL-6 in the serum of 12 uremic patients before and after hemodialysis and 10 controls were successfully detected." @default.
- W4213338045 created "2022-02-24" @default.
- W4213338045 creator A5052048534 @default.
- W4213338045 creator A5059205713 @default.
- W4213338045 date "2022-01-14" @default.
- W4213338045 modified "2023-10-18" @default.
- W4213338045 title "Detection of Interleukin-6 in the Serum of Uremia Patients Based on Fluorescence Image" @default.
- W4213338045 doi "https://doi.org/10.1109/iccece54139.2022.9712782" @default.
- W4213338045 hasPublicationYear "2022" @default.
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